AptaDB: Result

Interaction ID: 260

Aptamer sequence: TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT

Target unique ID: P08246

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_255	DNA-I	Protein	ELNE_HUMAN	17 nM	150 mM NaCl, 100 mM Tris HCl (pH 7.0), 2 mM MgCl2, 6 mM KCl	7479933

Aptamer Sequence:	TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT
The optimal secondary structure in dot-bracket notation:	..((((.(((((.(((.((..........)).)))))))).))))
The centroid secondary structure in dot-bracket notation:	..((((.(((((.(((.((..........)).)))))))).))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_255	Description	DNA-I
Aptamer chemistry	DNA	Length	45 nt
GC content	64.4%	Molecular weight	14,285.90 Da
Molarity of 1 μg/μl solution	70.00 μM	Number of G-quadruplexes	3
G-Score	64	Function	Targeted binding
Sequence	TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT
Applications	Vehicle for molecular delivery

Type	Detail	Type	Detail
Uniprot ID	P08246	Protein name	Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase)
Gene name(s)	ELANE ELA2	Organism	Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase)
Status	reviewed in uniprot database	Length	267
Mass	28,518	Activity regulation	N/A
Involvement in disease	Cyclic haematopoiesis (CH)	Catalytic activity	Reaction=Hydrolysis of proteins, including elastin. Preferential cleavage: Val-\|-Xaa > Ala-\|-Xaa
PDB ID(s)	1B0F;1H1B;1HNE;1PPF;1PPG;2RG3;2Z7F;3Q76;3Q77;4NZL;4WVP;5A09;5A0A;5A0B;5A0C;5A8X;5A8Y;5A8Z;5ABW;6E69;6F5M;6SMA;7CBK;
Function	Modifies the functions of natural killer cells, monocytes and granulocytes.

	Interaction ID	260
	Target type	Protein
	Target unique ID	P08246
	Activity	17 nM
	Binding Conditions/Buffer	150 mM NaCl, 100 mM Tris HCl (pH 7.0), 2 mM MgCl2, 6 mM KCl
	Assay	Binding curves of peptide-conjugated (solid circles; Kd = 7 ± 2 nM) and unconjugated (open circles; Kd = 17 ± 6 nM) DNA-I obtained by nitrocellulose filter binding
	PubMed ID	7479933

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_266	DNA	TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT	62.22%
Apta_862	DNA	TGTGCCAAAGAGAGTGGTGGGGGGGTGGGCGGAACTCGCG	62.22%
Apta_869	DNA	ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG	60.00%
Apta_1014	DNA	TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT	60.00%
Apta_942	DNA	AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA	58.00%
Apta_41	DNA	CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC	57.78%
Apta_69	DNA	AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT	57.78%
Apta_70	DNA	AGGTCGTAGGTTGGTGTGGTTGGGGCGACCT	57.78%
Apta_75	DNA	CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG	57.78%
Apta_364	DNA	TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT	57.78%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	57.78%
Apta_750	DNA	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT	57.78%
Apta_831	DNA	CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG	57.78%
Apta_880	DNA	GCCGCACAATCGGGTTGGGCAGGGAGGGCACGTGTGGCGC	57.78%
Apta_938	DNA	TTGCCCACCTCCCTGTGCGGGCCACAGAGCAGCAGTGTCGTG	57.78%
Apta_1094	DNA	GGAATCGTGGGTGGTAGGGTAGGGGATGCA	57.78%
Apta_1261	DNA	GGGCGGACGCTAGGTGGTGATGCTGTGCTACACGTGTTGT	57.78%
Apta_1035	DNA	ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC	56.67%
Apta_509	DNA	TCGCGCGAGTCGTCTGGGGGACTAGAGGACTTGTGCGGCCCCGCATCGTCCTCCC	56.36%
Apta_1071	DNA	TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT	56.14%

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