Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 929
Aptamer sequence: TGAGCCCAAGCCCTGGTATGTCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCAGGCAGGTCTACTTTGGGATC
Target unique ID: BAA-2587-PACK
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_843 Apt-8 Cell Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) 24.68 nM 1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2 29756774
Structure information of aptamer
Aptamer Sequence: TGAGCCCAAGCCCTGGTATGTCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCAGGCAGGTCTACTTTGGGATC
The optimal secondary structure in dot-bracket notation: ....((((((((..((..(((......((.((((.....)))).)).....)))..))..))).........)))))...
The centroid secondary structure in dot-bracket notation: ....(((((.....((..........))............................................)))))...
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_843 Description Apt-8
Aptamer chemistry DNA Length 80 nt
GC content 56.3% Molecular weight 24,595.69 Da
Molarity of 1 μg/μl solution 40.66 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted binding
Sequence TGAGCCCAAGCCCTGGTATGTCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCAGGCAGGTCTACTTTGGGATC
Applications Binding to Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Target information
Type Detail Type Detail
ATCC ID BAA-2587-PACK Cell name Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Category Bacteria Organism N/A
Product type Reporter-labeled bacterium Cell type N/A
Morphology N/A Tissue N/A
Disease N/A Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description A kit containing reporter-labeled Escherichia coli designed to distinguish control strain cross-contamination from true contamination in test samples. This product is appropriate for use as a positive control in quality control assays for E. coli.
Application(s) Assay development; Food testing; High-throughput screening
Activity data

No relevant experimental diagram

 Interaction ID 929
Target type Cell
Target unique ID BAA-2587-PACK
Activity 24.68 nM
Binding Conditions/Buffer

1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2
Assay

N/A
PubMed ID 29756774
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_842 DNA TGAGCCCAAGCCCTGGTATGTGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGGGGCAGGTCTACTTTGGGATC 75.00%
Apta_836 DNA TGAGCCCAAGCCCTGGTATGTTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGGGGCAGGTCTACTTTGGGATC 71.25%
Apta_838 DNA TGAGCCCAAGCCCTGGTATGTAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGGGGCAGGTCTACTTTGGGATC 71.25%
Apta_840 DNA TGAGCCCAAGCCCTGGTATGCGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTTGGCAGGTCTACTTTGGGATC 71.25%
Apta_844 DNA TGAGCCCAAGCCCTGGTATGTTGCGGGAGTCTAGCGGGCCACACTTTTATAGGTTCGCAGGGCAGGTCTACTTTGGGATC 71.25%
Apta_837 DNA TGAGCCCAAGCCCTGGTATGTGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAGGGCAGGTCTACTTTGGGATC 70.00%
Apta_839 DNA TGAGCCCAAGCCCTGGTATGAGCCCACGGAACACTGGTCGCGCCCACTGGTTTCTATATTGGCAGGTCTACTTTGGGATC 70.00%
Apta_841 DNA TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC 68.75%
Apta_104 DNA AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA 52.50%
Apta_104 DNA AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA 52.50%
Apta_405 DNA TCTAGCCCACTGCATAGAGGTAGCCACGTATGTGGGTGTTACCCATTCGTAGCTACCGGTG 52.50%
Apta_891 DNA GGAGACCGTACCATCTGTTCGTGGAAGCGCTTTGCTCGTCCATTAGCCTTGTGCTCGTGC 52.50%
Apta_994 DNA GCAATGGTACGGTACTTCCGGACAGTGCTGAAAACTGTGACCCCCCAAAAGTGCACGCTACTTTGCTAA 52.50%
Apta_1172 DNA GACGCCACTGACTACCCCAGTATCTCTTCAACGCGTATTGAAGTCAGTCGGTCGTCA 52.50%
Apta_1235 DNA TGTACCGTCTGAGCGATTCGTACGATCGGGTCGGGGTCTATTTTAGGGCCATTTGGCAGCCAGTCAGTGTTAAGGAGTGC 52.50%
Apta_587 DNA GGAATGGATCCACATCCATGGATGGGCAATGCGGGGTGGAGAATGGTTGCCGCACTTCGGCTTCACTGCAGACTTGACGAAGCTT 51.76%
Apta_466 DNA AGCTGACACAGCAGGTTGGTGGGGGTGGCTCACATCATGGTAGAATGACTGAACAGCGTTGATTAAAAGGCACGAGTCGAGCAATCTCGAAAT 51.61%
Apta_174 DNA AGCAGCACAGAGGTCAGATGTCGGTCTTGTGTATACATACCCGTGTGTTTTCATCTCAGGCCTATGCGTGCTACCGTGAA 51.25%
Apta_228 DNA AGCAGCACAGAGGTCAGATGCGGCGCGGGGTCTTTTACAAATAGTTGTCCTAGCCACGCCTATGCGTGCTACCGTGAA 51.25%
Apta_408 DNA GCAATGGTACGGTACTTCCGTCATCAGCTTGTGATGTGGATGCGAACTGCAAAAGTGCACGCTACTTTGCTAA 51.25%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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