Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 64
Aptamer sequence: ACCGTGTGGAGGGTGTT
Target unique ID: P00734
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_64 30-38[27] Protein THRB_HUMAN 42 nM 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM MgCl2 9368651
Structure information of aptamer
Aptamer Sequence: ACCGTGTGGAGGGTGTT
The optimal secondary structure in dot-bracket notation: (((........)))...
The centroid secondary structure in dot-bracket notation: (((........)))...
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_64 Description 30-38[27]
Aptamer chemistry DNA Length 17 nt
GC content 58.8% Molecular weight 5,297.43 Da
Molarity of 1 μg/μl solution 188.77 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted binding
Sequence ACCGTGTGGAGGGTGTT
Applications Inhibits thrombin-catalyzed fibrinclot formation in vitro
Target information
Type Detail Type Detail
Uniprot ID P00734 Protein name Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
Gene name(s) F2 Organism Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
Status reviewed in uniprot database Length 622
Mass 70,037 Activity regulation Inhibited by SERPINA5
Involvement in disease Factor II deficiency (FA2D) Catalytic activity Reaction=Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B
PDB ID(s)
1A2C;1A3B;1A3E;1A46;1A4W;1A5G;1A61;1ABI;1ABJ;1AD8;1AE8;1AFE;1AHT;1AI8;1AIX;1AWF;1AWH;1AY6;1B5G;1B7X;1BA8;1BB0;1BCU;1BHX;1BMM;1BMN;1BTH;1C1U;1C1V;1C1W;1C4U;1C4V;1C4Y;1C5L;1C5N;1C5O;1CA8;1D3D;1D3P;1D3Q;1D3T;1D4P;1D6W;1D9I;1DE7;1DIT;1DM4;1DOJ;1DWB;1DWC;1DWD;1DWE;1DX5;1E0F;1EB1;1EOJ;1EOL;1FPC;1FPH;1G30;1G32;1G37;1GHV;1GHW;1GHX;1GHY;1GJ4;1GJ5;1H8D;1H8I;1HAG;1HAH;1HAI;1HAO;1HAP;1HBT;1HDT;1HGT;1HLT;1HUT;1HXE;1HXF;1IHS;1IHT;1JMO;1JOU;1JWT;1K21;1K22;1KTS;1KTT;1LHC;1LHD;1LHE;1LHF;1LHG;1MH0;1MU6;1MU8;1MUE;1NM6;1NO9;1NRN;1NRO;1NRP;1NRQ;1NRR;1NRS;1NT1;1NU7;1NU9;1NY2;1NZQ;1O0D;1O2G;1O5G;1OOK;1OYT;1P8V;1PPB;1QBV;1QHR;1QJ1;1QJ6;1QJ7;1QUR;1RD3;1RIW;1SB1;1SFQ;1SG8;1SGI;1SHH;1SL3;1SR5;1T4U;1T4V;1TA2;1TA6;1TB6;1TBZ;1THP;1THR;1THS;1TMB;1TMT;1TMU;1TOM;1TQ0;1TQ7;1TWX;1UMA;1UVS;1VR1;1VZQ;1W7G;1WAY;1WBG;1XM1;1XMN;1YPE;1YPG;1YPJ;1YPK;1YPL;1YPM;1Z71;1Z8I;1Z8J;1ZGI;1ZGV;1ZRB;2A0Q;2A2X;2A45;2AFQ;2ANK;2ANM;2B5T;2BDY;2BVR;2BVS;2BVX;2BXT;2BXU;2C8W;2C8X;2C8Y;2C8Z;2C90;2C93;2CF8;2CF9;2CN0;2FEQ;2FES;2GDE;2GP9;2H9T;2HGT;2HNT;2HPP;2HPQ;2HWL;2JH0;2JH5;2JH6;2OD3;2PGB;2PGQ;2PKS;2PW8;2R2M;2THF;2UUF;2UUJ;2UUK;2V3H;2V3O;2ZC9;2ZDA;2ZDV;2ZF0;2ZFF;2ZFP;2ZFQ;2ZFR;2ZG0;2ZGB;2ZGX;2ZHE;2ZHF;2ZHQ;2ZHW;2ZI2;2ZIQ;2ZNK;2ZO3;3B23;3B9F;3BEF;3BEI;3BF6;3BIU;3BIV;3BV9;3C1K;3C27;3D49;3DA9;3DD2;3DHK;3DT0;3DUX;3E6P;3EE0;3EGK;3EQ0;3F68;3GIC;3GIS;3HAT;3HKJ;3HTC;3JZ1;3JZ2;3K65;3LDX;3LU9;3NXP;3P17;3P6Z;3P70;3PMH;3PO1;3QDZ;3QGN;3QLP;3QTO;3QTV;3QWC;3QX5;3R3G;3RLW;3RLY;3RM0;3RM2;3RML;3RMM;3RMN;3RMO;3S7H;3S7K;3SHA;3SHC;3SI3;3SI4;3SQE;3SQH;3SV2;3T5F;3TU7;3U69;3U8O;3U8R;3U8T;3U98;3U9A;3UTU;3UWJ;3VXE;3VXF;4AX9;4AYV;4AYY;4AZ2;4BAH;4BAK;4BAM;4BAN;4BAO;4BAQ;4BOH;4CH2;4CH8;4DIH;4DII;4DT7;4DY7;4E05;4E06;4E7R;4H6S;4H6T;4HFP;4HTC;4HZH;4I7Y;4LOY;4LXB;4LZ1;4LZ4;4MLF;4NZQ;4O03;4RKJ;4RKO;4RN6;4THN;4UD9;4UDW;4UE7;4UEH;4UFD;4UFE;4UFF;4UFG;4YES;5A2M;5AF9;5AFY;5AFZ;5AHG;5CMX;5DO4;5E8E;5EDK;5EDM;5EW1;5EW2;5GDS;5GIM;5JDU;5JFD;5JZY;5L6N;5MJT;5MLS;5MM6;5NHU;5TO3;5Z5W;5Z5X;6BJR;6C2W;6EO6;6EO7;6EO8;6EO9;6V5T;7KME;8KME;
Function Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C.
Activity data

No relevant experimental diagram

 Interaction ID 64
Target type Protein
Target unique ID P00734
Activity 42 nM
Binding Conditions/Buffer

50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM MgCl2
Assay

N/A
PubMed ID 9368651
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_66 DNA TACCGTGGTAGGGAAGGTTGGAGTGTA 59.26%
Apta_1038 DNA CCCGTCCGTATGGTGGG 58.82%
Apta_1039 DNA GCCCGTCCGTATGGTGGGT 57.89%
Apta_546 DNA CACGGCATGGTGGGCGTCGTG 57.14%
Apta_669 DNA AACGTGGGAGGGCGGTGGTGTTGAA 56.00%
Apta_62 DNA AGTCCGTGGTAGGGCAGGTTGGGGTGACT 55.17%
Apta_63 DNA GACCCGTGGTAGGGTAGGATGGGGTGGTC 55.17%
Apta_65 DNA CTACCGTGGTAGGGAAGGTTGGAGTGTAG 55.17%
Apta_483 DNA ATTGGGAGGGATTGGGTGGG 55.00%
Apta_389 DNA GCCTGTGGTGTTGGGGCGGGTGCG 54.17%
Apta_1019 DNA ATCCCTGCAGTTTGGTAGGGTGCAGG 53.85%
Apta_245 DNA ACAGGGGTGTGGGGACAGGGGTGTGGGG 53.57%
Apta_320 DNA GCACTCTGTGGGGGTGGACGGGCCGGGT 53.57%
Apta_675 DNA ACTGTCCGTCGGGTTTAGGGTGGCGTTCGG 53.33%
Apta_1021 DNA CTGTTTGGTAGGCAG 52.94%
Apta_757 DNA GGTGGTGGTGGTTGTGGTGGTGG 52.17%
Apta_630 DNA GACCAGGGCAAACGGTAGGGAGTGGTC 51.85%
Apta_702 DNA TACCCGGCGTTCGGGGGGTACCGCTGC 51.85%
Apta_141 DNA GTTGACGGGCGATTGGTCTT 50.00%
Apta_210 DNA AGGTTGGTTGGTTGGAAT 50.00%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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