AptaDB: Result

Interaction ID: 483

Aptamer sequence: CTTCACTTTCGTGGACGGTGGCGTCGGGGCGGTTTGTATG

Target unique ID: Q61727

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_478	R5Cw4	Protein	I10R1_MOUSE	19 nM	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature	22434135

Aptamer Sequence:	CTTCACTTTCGTGGACGGTGGCGTCGGGGCGGTTTGTATG
The optimal secondary structure in dot-bracket notation:	........((((.((((....))))...))))........
The centroid secondary structure in dot-bracket notation:	.............((((....))))...............

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_478	Description	R5Cw4
Aptamer chemistry	DNA	Length	40 nt
GC content	60.0%	Molecular weight	12,412.92 Da
Molarity of 1 μg/μl solution	80.56 μM	Number of G-quadruplexes	2
G-Score	19	Function	Targeted therapy
Sequence	CTTCACTTTCGTGGACGGTGGCGTCGGGGCGGTTTGTATG
Applications	Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro

Type	Detail	Type	Detail
Uniprot ID	Q61727	Protein name	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Gene name(s)	Il10ra Il10r	Organism	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Status	reviewed in uniprot database	Length	575
Mass	64,248	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation.

No relevant experimental diagram	Interaction ID	483
	Target type	Protein
	Target unique ID	Q61727
	Activity	19 nM
	Binding Conditions/Buffer	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature
	Assay	N/A
	PubMed ID	22434135

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1081	DNA	CTTACGACTGTGGTCGGGTGGTGGGCCTCTAGAGGGGTGTCGTAAG	65.22%
Apta_476	DNA	CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG	62.50%
Apta_475	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGGTCGGTTTGTATG	60.00%
Apta_652	DNA	CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGGCAGGGGGTGTGCGTG	58.18%
Apta_320	DNA	GCACTCTGTGGGGGTGGACGGGCCGGGT	57.50%
Apta_474	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGATCGGTTTGTATG	57.50%
Apta_577	DNA	ACTTCAGTGAGTTGTCCCACGGTCGGCGAGTCGGTGGTAG	57.50%
Apta_681	DNA	CCTGACGATCGGGTGTGGGTTGGCTTGAGG	57.50%
Apta_1014	DNA	TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT	57.50%
Apta_1085	DNA	TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC	57.50%
Apta_1080	DNA	CTTACGACCCAGGGGGGTGGACAGGCGGGGGTTAGGGGGGTCGTAAG	57.45%
Apta_651	DNA	CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG	55.36%
Apta_12	DNA	TGAGGGGGTGGGTGGGACGGGTTGG	55.00%
Apta_65	DNA	CTACCGTGGTAGGGAAGGTTGGAGTGTAG	55.00%
Apta_148	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG	55.00%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	55.00%
Apta_649	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG	55.00%
Apta_683	DNA	GCAGTACGATCGGGGGTGGGTGGATGTAGG	55.00%
Apta_692	DNA	ACGTTGACGCTGGTGCCCGCTTGTGGTGCGAGTGTTGTGT	55.00%
Apta_344	DNA	CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG	54.17%

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