Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1021
Aptamer sequence: GACGCTTACTCAGGTGTGACTCGGGGTGTAGCTCGTTATGATTCGGACAAGACTTACCTTGCGCCTCTGGGATCGAAGGACGCAGATGAAGTCTC
Target unique ID: Other-37
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_933 | GVI-1 | Other | GCRV-infected CIK cell | 220.86 nM | 10% FBS | 32959408 |
Structure information of aptamer
| Aptamer Sequence: | GACGCTTACTCAGGTGTGACTCGGGGTGTAGCTCGTTATGATTCGGACAAGACTTACCTTGCGCCTCTGGGATCGAAGGACGCAGATGAAGTCTC |
| The optimal secondary structure in dot-bracket notation: | ((.((((((((.(((...)))..))))).)))))(((........))).((((((...((((((((.((....)).))).)))))...)))))). |
| The centroid secondary structure in dot-bracket notation: | ((.((((((((............))))).)))))...............((((((...((((((((.((....)).))).)))))...)))))). |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_933 | Description | GVI-1 |
| Aptamer chemistry | DNA | Length | 95 nt |
| GC content | 53.7% | Molecular weight | 29,411.78 Da |
| Molarity of 1 μg/μl solution | 34.00 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | GACGCTTACTCAGGTGTGACTCGGGGTGTAGCTCGTTATGATTCGGACAAGACTTACCTTGCGCCTCTGGGATCGAAGGACGCAGATGAAGTCTC | ||
| Applications | Recognize and bind to GCRV-infected cells | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-37 | Name | GCRV-infected CIK cell |
Activity data
 |
Interaction ID | 1021 |
| Target type | Other | |
| Target unique ID | Other-37 | |
| Activity | 220.86 nM | |
| Binding Conditions/Buffer | 10% FBS |
|
| Assay | Apparent equilibrium dissociation constant (Kd) of each aptamer binding to target GCRV-CIK cells. The concentrations of FAM-labelled aptamer candidates were diluted to 0-1,000 nM in PBS, respectively, and then incubated with target GCRV-CIK cells (5 × 10**5) at 4°C for 30 min. The binding fluorescence intensities of aptamer-target cells were detected by flow cytometry. Kd of each aptamer candidate was calculated according to the equation Y = BmaxX/(Kd + X) by SigmaPlot software |
|
| PubMed ID | 32959408 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_935 | DNA | GACGCTTACTCAGGTGTGACTCGTCCGTCGGTTCGCCTTGCGAGTGCACGCCGCCAGCCGTTACATAAAGTCTGCCCACTCGAAGGACGCAGATGAAGTCTC | |
| Apta_934 | DNA | GACGCTTACTCAGGTGTGACTCGTGAACCCACCTCAGGGCATCTTACATTTCTTCTAAGTTGTTACCATGTTTCGAAGGACGCAGATGAAGTCTC | |
| Apta_625 | DNA | ATACCAGCTTATTCAATTCAGGGCGGTATGAGGCTCGATCAAGGTCGGAGCGAGAATTTTTTCGCGGAGTCGGCTGGATCAGATAGTAAGTGCAATCT | |
| Apta_625 | DNA | ATACCAGCTTATTCAATTCAGGGCGGTATGAGGCTCGATCAAGGTCGGAGCGAGAATTTTTTCGCGGAGTCGGCTGGATCAGATAGTAAGTGCAATCT | |
| Apta_704 | DNA | GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCGAGCATGGAGTCGGATCCTCTA | |
| Apta_451 | DNA | ATACCAGCTTATTCAATTGAGTAAGAGTGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCAATCT | |
| Apta_772 | DNA | GAATTCAGTCGGACAGCGCGGTGTTGGTGGTGGGGTGGTTTGGGAATCTGTTCGGCTGGAGATGGACGAATATCGTCTCCC | |
| Apta_226 | DNA | GGGAGATCACTTACGGCACCGTATAGTCGTACATGAACGAGTGTGAGTGTTAAGCCTTTGGCGACAGGGCTCGGAACC | |
| Apta_349 | DNA | ATACCAGCTTATTCAATTAGACGAGTTTTATTCATTTTCCATGTCGTAGCTTACCGTGAGATAGTAAGTGCAATCT | |
| Apta_769 | DNA | GAATTCAGTCGGACAGCGAGTTGTTGTTAGGGTGTTGGGTTGGGGTTTTAGGGTCCCTGTCGTCGATGGACGAATATCGTCTCCC | |
| Apta_774 | DNA | GAATTCAGTCGGACAGCGAGGAAAATTTCGTTATTTTCGTTTCCGGGAACTCGGGCATTTAGAGATGGACGAATATCGTCTCCC | |
| Apta_775 | DNA | GAATTCAGTCGGACAGCGCGGTGTTGGTGGCGGGGGTGGTTGGGGGATTTGGCTGTCCTGAGTGATGGACGAATATCGTCTCCC | |
| Apta_1015 | DNA | ATACCAGCTTATTCAATTGACCCGTTTCGTTCCCTCTGGGAAGTTTAGCCCAGTTGCCTGGGCGATACCAAGATAGTAAGTGCAATCT | |
| Apta_1179 | DNA | GAATTCAGTCGGACAGCGAAGTAGTTTTCCTTCTAACCTAAGAACCCGCGGCAGTTTAATGTAGATGGACGAATACGTCTCCC | |
| Apta_1184 | DNA | GAATTCAGTCGGACAGCGTAATCCCTTGTGTTTGACGTTCGAAGATTATTTCTATCTTTTAGGATGGACGAATATCGTCTCCC | |
| Apta_627 | DNA | ATACCAGCTTATTCAATTATGCGGGGATGAGGCTTGATCTGTGGCTCTGATCCATGATCAGTCGTCTTACGTGCGGTCCAGATAGTAAGTGCAATCT | |
| Apta_627 | DNA | ATACCAGCTTATTCAATTATGCGGGGATGAGGCTTGATCTGTGGCTCTGATCCATGATCAGTCGTCTTACGTGCGGTCCAGATAGTAAGTGCAATCT | |
| Apta_281 | DNA | CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT | |
| Apta_336 | DNA | CATGCTTCCCCAGGGAGATGACCGGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTGG | |
| Apta_351 | DNA | TTCTCGGTTGGTCTCTGGCGGAGCGATCATCTCAGAGCATTCTTAGCGTTTTGTTCTTGTGTATGATTCGCTTTTCCC |
DEV TOOL
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