Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 62
Aptamer sequence: AGTCCGTGGTAGGGCAGGTTGGGGTGACT
Target unique ID: P00734
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_62 60-18 Protein THRB_HUMAN 0.5 nM 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM MgCl2 9368651
Structure information of aptamer
Aptamer Sequence: AGTCCGTGGTAGGGCAGGTTGGGGTGACT
The optimal secondary structure in dot-bracket notation: .((((......))))..............
The centroid secondary structure in dot-bracket notation: .((((......))))..(((.....))).
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_62 Description 60-18
Aptamer chemistry DNA Length 29 nt
GC content 57.7% Molecular weight 24,339.58 Da
Molarity of 1 μg/μl solution 41.09 μM Number of G-quadruplexes 2
G-Score 20 Function Targeted binding
Sequence AGTCCGTGGTAGGGCAGGTTGGGGTGACT
Applications Inhibits thrombin-catalyzed fibrinclot formation in vitro
Target information
Type Detail Type Detail
Uniprot ID P00734 Protein name Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
Gene name(s) F2 Organism Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
Status reviewed in uniprot database Length 622
Mass 70,037 Activity regulation Inhibited by SERPINA5
Involvement in disease Factor II deficiency (FA2D) Catalytic activity Reaction=Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B
PDB ID(s)
1A2C;1A3B;1A3E;1A46;1A4W;1A5G;1A61;1ABI;1ABJ;1AD8;1AE8;1AFE;1AHT;1AI8;1AIX;1AWF;1AWH;1AY6;1B5G;1B7X;1BA8;1BB0;1BCU;1BHX;1BMM;1BMN;1BTH;1C1U;1C1V;1C1W;1C4U;1C4V;1C4Y;1C5L;1C5N;1C5O;1CA8;1D3D;1D3P;1D3Q;1D3T;1D4P;1D6W;1D9I;1DE7;1DIT;1DM4;1DOJ;1DWB;1DWC;1DWD;1DWE;1DX5;1E0F;1EB1;1EOJ;1EOL;1FPC;1FPH;1G30;1G32;1G37;1GHV;1GHW;1GHX;1GHY;1GJ4;1GJ5;1H8D;1H8I;1HAG;1HAH;1HAI;1HAO;1HAP;1HBT;1HDT;1HGT;1HLT;1HUT;1HXE;1HXF;1IHS;1IHT;1JMO;1JOU;1JWT;1K21;1K22;1KTS;1KTT;1LHC;1LHD;1LHE;1LHF;1LHG;1MH0;1MU6;1MU8;1MUE;1NM6;1NO9;1NRN;1NRO;1NRP;1NRQ;1NRR;1NRS;1NT1;1NU7;1NU9;1NY2;1NZQ;1O0D;1O2G;1O5G;1OOK;1OYT;1P8V;1PPB;1QBV;1QHR;1QJ1;1QJ6;1QJ7;1QUR;1RD3;1RIW;1SB1;1SFQ;1SG8;1SGI;1SHH;1SL3;1SR5;1T4U;1T4V;1TA2;1TA6;1TB6;1TBZ;1THP;1THR;1THS;1TMB;1TMT;1TMU;1TOM;1TQ0;1TQ7;1TWX;1UMA;1UVS;1VR1;1VZQ;1W7G;1WAY;1WBG;1XM1;1XMN;1YPE;1YPG;1YPJ;1YPK;1YPL;1YPM;1Z71;1Z8I;1Z8J;1ZGI;1ZGV;1ZRB;2A0Q;2A2X;2A45;2AFQ;2ANK;2ANM;2B5T;2BDY;2BVR;2BVS;2BVX;2BXT;2BXU;2C8W;2C8X;2C8Y;2C8Z;2C90;2C93;2CF8;2CF9;2CN0;2FEQ;2FES;2GDE;2GP9;2H9T;2HGT;2HNT;2HPP;2HPQ;2HWL;2JH0;2JH5;2JH6;2OD3;2PGB;2PGQ;2PKS;2PW8;2R2M;2THF;2UUF;2UUJ;2UUK;2V3H;2V3O;2ZC9;2ZDA;2ZDV;2ZF0;2ZFF;2ZFP;2ZFQ;2ZFR;2ZG0;2ZGB;2ZGX;2ZHE;2ZHF;2ZHQ;2ZHW;2ZI2;2ZIQ;2ZNK;2ZO3;3B23;3B9F;3BEF;3BEI;3BF6;3BIU;3BIV;3BV9;3C1K;3C27;3D49;3DA9;3DD2;3DHK;3DT0;3DUX;3E6P;3EE0;3EGK;3EQ0;3F68;3GIC;3GIS;3HAT;3HKJ;3HTC;3JZ1;3JZ2;3K65;3LDX;3LU9;3NXP;3P17;3P6Z;3P70;3PMH;3PO1;3QDZ;3QGN;3QLP;3QTO;3QTV;3QWC;3QX5;3R3G;3RLW;3RLY;3RM0;3RM2;3RML;3RMM;3RMN;3RMO;3S7H;3S7K;3SHA;3SHC;3SI3;3SI4;3SQE;3SQH;3SV2;3T5F;3TU7;3U69;3U8O;3U8R;3U8T;3U98;3U9A;3UTU;3UWJ;3VXE;3VXF;4AX9;4AYV;4AYY;4AZ2;4BAH;4BAK;4BAM;4BAN;4BAO;4BAQ;4BOH;4CH2;4CH8;4DIH;4DII;4DT7;4DY7;4E05;4E06;4E7R;4H6S;4H6T;4HFP;4HTC;4HZH;4I7Y;4LOY;4LXB;4LZ1;4LZ4;4MLF;4NZQ;4O03;4RKJ;4RKO;4RN6;4THN;4UD9;4UDW;4UE7;4UEH;4UFD;4UFE;4UFF;4UFG;4YES;5A2M;5AF9;5AFY;5AFZ;5AHG;5CMX;5DO4;5E8E;5EDK;5EDM;5EW1;5EW2;5GDS;5GIM;5JDU;5JFD;5JZY;5L6N;5MJT;5MLS;5MM6;5NHU;5TO3;5Z5W;5Z5X;6BJR;6C2W;6EO6;6EO7;6EO8;6EO9;6V5T;7KME;8KME;
Function Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C.
Activity data

No relevant experimental diagram

 Interaction ID 62
Target type Protein
Target unique ID P00734
Activity 0.5 nM
Binding Conditions/Buffer

50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM MgCl2
Assay

N/A
PubMed ID 9368651
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_72 DNA AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT 77.42%
Apta_63 DNA GACCCGTGGTAGGGTAGGATGGGGTGGTC 72.41%
Apta_65 DNA CTACCGTGGTAGGGAAGGTTGGAGTGTAG 72.41%
Apta_66 DNA TACCGTGGTAGGGAAGGTTGGAGTGTA 72.41%
Apta_70 DNA AGGTCGTAGGTTGGTGTGGTTGGGGCGACCT 70.97%
Apta_669 DNA AACGTGGGAGGGCGGTGGTGTTGAA 65.52%
Apta_69 DNA AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT 64.52%
Apta_74 DNA GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC 64.52%
Apta_22 DNA TGTGGGGGTGGACTGGGTGGGTACC 62.07%
Apta_1149 DNA ATCATGGTGGGTATCGGCACTCGTTGGTTGAT 59.38%
Apta_218 RNA AGCCGCGAGGGGAGGGAUAGGGUAGGGCGCGGCU 58.82%
Apta_14 DNA AGCGGGGGGGGCTGGGCTGGGTGGG 58.62%
Apta_21 DNA TGTGGGGGAGGACGGGCGGGGATGC 58.62%
Apta_483 DNA ATTGGGAGGGATTGGGTGGG 58.62%
Apta_71 DNA GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC 58.06%
Apta_73 DNA GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC 58.06%
Apta_75 DNA CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG 58.06%
Apta_124 DNA GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC 56.67%
Apta_1094 DNA GGAATCGTGGGTGGTAGGGTAGGGGATGCA 56.67%
Apta_41 DNA CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC 56.41%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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