Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 66
Aptamer sequence: TACCGTGGTAGGGAAGGTTGGAGTGTA
Target unique ID: P00734
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_66 30-16[27] Protein THRB_HUMAN 126 nM 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM MgCl2 9368651
Structure information of aptamer
Aptamer Sequence: TACCGTGGTAGGGAAGGTTGGAGTGTA
The optimal secondary structure in dot-bracket notation: ..((......))...............
The centroid secondary structure in dot-bracket notation: ..((......))...............
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_66 Description 30-16[27]
Aptamer chemistry DNA Length 27 nt
GC content 51.9% Molecular weight 8,715.40 Da
Molarity of 1 μg/μl solution 114.74 μM Number of G-quadruplexes 2
G-Score 20 Function Targeted binding
Sequence TACCGTGGTAGGGAAGGTTGGAGTGTA
Applications Inhibits thrombin-catalyzed fibrinclot formation in vitro
Target information
Type Detail Type Detail
Uniprot ID P00734 Protein name Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
Gene name(s) F2 Organism Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
Status reviewed in uniprot database Length 622
Mass 70,037 Activity regulation Inhibited by SERPINA5
Involvement in disease Factor II deficiency (FA2D) Catalytic activity Reaction=Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B
PDB ID(s)
1A2C;1A3B;1A3E;1A46;1A4W;1A5G;1A61;1ABI;1ABJ;1AD8;1AE8;1AFE;1AHT;1AI8;1AIX;1AWF;1AWH;1AY6;1B5G;1B7X;1BA8;1BB0;1BCU;1BHX;1BMM;1BMN;1BTH;1C1U;1C1V;1C1W;1C4U;1C4V;1C4Y;1C5L;1C5N;1C5O;1CA8;1D3D;1D3P;1D3Q;1D3T;1D4P;1D6W;1D9I;1DE7;1DIT;1DM4;1DOJ;1DWB;1DWC;1DWD;1DWE;1DX5;1E0F;1EB1;1EOJ;1EOL;1FPC;1FPH;1G30;1G32;1G37;1GHV;1GHW;1GHX;1GHY;1GJ4;1GJ5;1H8D;1H8I;1HAG;1HAH;1HAI;1HAO;1HAP;1HBT;1HDT;1HGT;1HLT;1HUT;1HXE;1HXF;1IHS;1IHT;1JMO;1JOU;1JWT;1K21;1K22;1KTS;1KTT;1LHC;1LHD;1LHE;1LHF;1LHG;1MH0;1MU6;1MU8;1MUE;1NM6;1NO9;1NRN;1NRO;1NRP;1NRQ;1NRR;1NRS;1NT1;1NU7;1NU9;1NY2;1NZQ;1O0D;1O2G;1O5G;1OOK;1OYT;1P8V;1PPB;1QBV;1QHR;1QJ1;1QJ6;1QJ7;1QUR;1RD3;1RIW;1SB1;1SFQ;1SG8;1SGI;1SHH;1SL3;1SR5;1T4U;1T4V;1TA2;1TA6;1TB6;1TBZ;1THP;1THR;1THS;1TMB;1TMT;1TMU;1TOM;1TQ0;1TQ7;1TWX;1UMA;1UVS;1VR1;1VZQ;1W7G;1WAY;1WBG;1XM1;1XMN;1YPE;1YPG;1YPJ;1YPK;1YPL;1YPM;1Z71;1Z8I;1Z8J;1ZGI;1ZGV;1ZRB;2A0Q;2A2X;2A45;2AFQ;2ANK;2ANM;2B5T;2BDY;2BVR;2BVS;2BVX;2BXT;2BXU;2C8W;2C8X;2C8Y;2C8Z;2C90;2C93;2CF8;2CF9;2CN0;2FEQ;2FES;2GDE;2GP9;2H9T;2HGT;2HNT;2HPP;2HPQ;2HWL;2JH0;2JH5;2JH6;2OD3;2PGB;2PGQ;2PKS;2PW8;2R2M;2THF;2UUF;2UUJ;2UUK;2V3H;2V3O;2ZC9;2ZDA;2ZDV;2ZF0;2ZFF;2ZFP;2ZFQ;2ZFR;2ZG0;2ZGB;2ZGX;2ZHE;2ZHF;2ZHQ;2ZHW;2ZI2;2ZIQ;2ZNK;2ZO3;3B23;3B9F;3BEF;3BEI;3BF6;3BIU;3BIV;3BV9;3C1K;3C27;3D49;3DA9;3DD2;3DHK;3DT0;3DUX;3E6P;3EE0;3EGK;3EQ0;3F68;3GIC;3GIS;3HAT;3HKJ;3HTC;3JZ1;3JZ2;3K65;3LDX;3LU9;3NXP;3P17;3P6Z;3P70;3PMH;3PO1;3QDZ;3QGN;3QLP;3QTO;3QTV;3QWC;3QX5;3R3G;3RLW;3RLY;3RM0;3RM2;3RML;3RMM;3RMN;3RMO;3S7H;3S7K;3SHA;3SHC;3SI3;3SI4;3SQE;3SQH;3SV2;3T5F;3TU7;3U69;3U8O;3U8R;3U8T;3U98;3U9A;3UTU;3UWJ;3VXE;3VXF;4AX9;4AYV;4AYY;4AZ2;4BAH;4BAK;4BAM;4BAN;4BAO;4BAQ;4BOH;4CH2;4CH8;4DIH;4DII;4DT7;4DY7;4E05;4E06;4E7R;4H6S;4H6T;4HFP;4HTC;4HZH;4I7Y;4LOY;4LXB;4LZ1;4LZ4;4MLF;4NZQ;4O03;4RKJ;4RKO;4RN6;4THN;4UD9;4UDW;4UE7;4UEH;4UFD;4UFE;4UFF;4UFG;4YES;5A2M;5AF9;5AFY;5AFZ;5AHG;5CMX;5DO4;5E8E;5EDK;5EDM;5EW1;5EW2;5GDS;5GIM;5JDU;5JFD;5JZY;5L6N;5MJT;5MLS;5MM6;5NHU;5TO3;5Z5W;5Z5X;6BJR;6C2W;6EO6;6EO7;6EO8;6EO9;6V5T;7KME;8KME;
Function Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C.
Activity data

No relevant experimental diagram

 Interaction ID 66
Target type Protein
Target unique ID P00734
Activity 126 nM
Binding Conditions/Buffer

50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM MgCl2
Assay

N/A
PubMed ID 9368651
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_65 DNA CTACCGTGGTAGGGAAGGTTGGAGTGTAG
93.10%
Apta_63 DNA GACCCGTGGTAGGGTAGGATGGGGTGGTC 75.86%
Apta_62 DNA AGTCCGTGGTAGGGCAGGTTGGGGTGACT 72.41%
Apta_669 DNA AACGTGGGAGGGCGGTGGTGTTGAA 66.67%
Apta_74 DNA GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC 61.29%
Apta_1094 DNA GGAATCGTGGGTGGTAGGGTAGGGGATGCA 60.00%
Apta_26 DNA AGGGGGGGGGGTAAGGGTGGGTAGA 59.26%
Apta_64 DNA ACCGTGTGGAGGGTGTT 59.26%
Apta_483 DNA ATTGGGAGGGATTGGGTGGG 59.26%
Apta_75 DNA CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG 58.06%
Apta_850 DNA CACCAGTGTGTTGAGGTTTGAGCACACTGATAGAGTGTCA 57.50%
Apta_1085 DNA TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC 56.67%
Apta_1150 DNA CTAAGCAGGAAGGTATGGATTGGTTGCATTAG 56.25%
Apta_13 DNA AGGGGGGGGGGAGGGGTCGGGGTGG 55.56%
Apta_14 DNA AGCGGGGGGGGCTGGGCTGGGTGGG 55.56%
Apta_16 DNA AGGGGGGGGGGACGGGACGGGTATA 55.56%
Apta_552 DNA CACCTGGGGGAGTATTGCGGAGGAAGGTTCCAGGTG 55.56%
Apta_630 DNA GACCAGGGCAAACGGTAGGGAGTGGTC 55.56%
Apta_806 DNA GGTTGGATGTAAGGTTGGAGGGGGG 55.56%
Apta_691 DNA CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT 55.00%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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