Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 141
Aptamer sequence: GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT
Target unique ID: P02741
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_140 | clone 1 | Protein | CRP_HUMAN | 3.51 nM | 10 mM Tris buffer with 50 mM NaCl and 2 mM CaCl2 | 20061133 |
Structure information of aptamer
| Aptamer Sequence: | GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT |
| The optimal secondary structure in dot-bracket notation: | ((((...((((...((((((......((((((((......))))).)))...))))))))))....)))).. |
| The centroid secondary structure in dot-bracket notation: | ((((...((((...((((((......((.(((((......)))))..))...))))))))))....)))).. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_140 | Description | clone 1 |
| Aptamer chemistry | DNA | Length | 72 nt |
| GC content | 51.4% | Molecular weight | 22,811.35 Da |
| Molarity of 1 μg/μl solution | 43.84 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT | ||
| Applications | Recognition molecule for the detection of CRP | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P02741 | Protein name | C-reactive protein [Cleaved into: C-reactive protein(1-205)] |
| Gene name(s) | CRP PTX1 | Organism | C-reactive protein [Cleaved into: C-reactive protein(1-205)] |
| Status | reviewed in uniprot database | Length | 224 |
| Mass | 25,039 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | 1B09;1GNH;1LJ7;3L2Y;3PVN;3PVO;7PK9;7PKB;7PKD;7PKE;7PKF;7PKG;7PKH; |
 |
Function | Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 141 |
| Target type | Protein | |
| Target unique ID | P02741 | |
| Activity | 3.51 nM | |
| Binding Conditions/Buffer | 10 mM Tris buffer with 50 mM NaCl and 2 mM CaCl2 |
|
| Assay | N/A |
|
| PubMed ID | 20061133 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_417 | DNA | GGCAGGAAGACAAACACCAGGTGAGGGAGACGACGCGAGTGTTAGATGGTAGCTGTTGGTCTGTGGTGCTGT | |
| Apta_418 | DNA | TGGCAGGAAGACAAACACATCGTCGCGGCCTTAGGAGGGGCGGACGGGGGGGGGCGTGGTCTGTGGTGCTGT | |
| Apta_887 | DNA | ACGCTCGGATGCCACTACAGGGGGGTGGTCCTGAGGGTGGTGTGGTTGGTTTGGTTTCCTCATGGACGTGCTGGTGAC | |
| Apta_281 | DNA | CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT | |
| Apta_236 | DNA | AGCAGCACAGAGGTCAGATGAGATGGTTGACCGGTTGATGTGATTAAGGAGACGTCGATTCCTATGCGTGCTACCGTGAA | |
| Apta_764 | DNA | AGCAGAGTTCACGACCCGATAAGGGGAAAATACATTTCGGATCTCCGTTGTCGCCCTGATACCGAGTTACATACCAATCGTCGCAG | |
| Apta_650 | DNA | GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_756 | DNA | GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT | |
| Apta_1099 | DNA | ATCCATTGCCACTGACTACCAGCCGGGTGGGTGGGGGGGTTTGCTGGTATCGCATTGTGCGAAGTCAGTCGGTCGTTAGT | |
| Apta_454 | DNA | AGCTCCAGAAGATAAATTACAGGTGAGGGCGGGCGGGTGGTTGTAATATGATCGAATGGTATATGTGTGTTTGCAACTAGGATACTATGACCCCG | |
| Apta_761 | DNA | AGCAGAGTTCACGACCCGATAAGGGGAAAACACATTTCGGATCTCCGTTGTCGCCCTGATACCGAGTTACATACCAATCGTCGCAG | |
| Apta_765 | DNA | AGCAGAGTTCACGACCCGATAAGGGGAAAATACATTTCGGATCTCCGTTGTCGCCCTGGTACCGAGTTACATACCAATCGTCGCAG | |
| Apta_1134 | DNA | GCCGAGATTGCACTTACTATCTGTTGAGGGAAGAAGTGGTGTAATGTGGAATAATTGTTGTAATAGTAATTGATAAGCTGGTATGCGC | |
| Apta_223 | DNA | CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG | |
| Apta_464 | DNA | GTCAGATTCCACTATAGTAGGTTGGGTAGGGTGGTCGCAGTGGATGATATGTCGTAGGGG | |
| Apta_686 | DNA | GGTCACCAACAACAGGGAGCGCTACGCGAAGGGTCAATGTGACGTCATGCGGATGTGTGG | |
| Apta_753 | DNA | GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT | |
| Apta_755 | DNA | GGGGAGGGAGACACAGTCATGGAGCAGTTATTAGGGTGTACCGGGTGTAGT | |
| Apta_988 | DNA | AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG | |
| Apta_1230 | DNA | GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG |
DEV TOOL
 |
 |
 |
 |