Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 389
Aptamer sequence: GGCGUGACCUGAUGAGUCACGCUAAGGAGGAUUUCCGAAAGCGGCUACGGUCCGCCAGUGUUACGAAACGUUCCC
Target unique ID: P28482
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_383 | N | Protein | MK01_HUMAN | 2 nM | 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.05 μg/μl tRNA | 12118241 |
Structure information of aptamer
| Aptamer Sequence: | GGCGUGACCUGAUGAGUCACGCUAAGGAGGAUUUCCGAAAGCGGCUACGGUCCGCCAGUGUUACGAAACGUUCCC |
| The optimal secondary structure in dot-bracket notation: | ((((((((.......))))))))..((((..((((.....(((((........))).)).....))))..)))). |
| The centroid secondary structure in dot-bracket notation: | ((((((((.......))))))))..((((..((((.............................))))..)))). |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_383 | Description | N |
| Aptamer chemistry | RNA | Length | 75 nt |
| GC content | 57.3% | Molecular weight | 24,170.38 Da |
| Molarity of 1 μg/μl solution | 41.37 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | GGCGUGACCUGAUGAGUCACGCUAAGGAGGAUUUCCGAAAGCGGCUACGGUCCGCCAGUGUUACGAAACGUUCCC | ||
| Applications | Monitoring post-translational modification of proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P28482 | Protein name | Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2) |
| Gene name(s) | MAPK1 ERK2 PRKM1 PRKM2 | Organism | Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2) |
| Status | reviewed in uniprot database | Length | 360 |
| Mass | 41,390 | Activity regulation | Phosphorylated by MAP2K1/MEK1 and MAP2K2/MEK2 on Thr-185 and Tyr-187 in response to external stimuli like insulin or NGF |
| Involvement in disease | Noonan syndrome 13 (NS13) | Catalytic activity | Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein] |
| PDB ID(s) | 1PME;1TVO;1WZY;2OJG;2OJI;2OJJ;2Y9Q;3D42;3D44;3I5Z;3I60;3SA0;3TEI;3W55;4FMQ;4FUX;4FUY;4FV0;4FV1;4FV2;4FV3;4FV4;4FV5;4FV6;4FV7;4FV8;4FV9;4G6N;4G6O;4H3P;4H3Q;4IZ5;4IZ7;4IZA;4N0S;4NIF;4O6E;4QP1;4QP2;4QP3;4QP4;4QP6;4QP7;4QP8;4QP9;4QPA;4QTA;4QTE;4XJ0;4ZXT;4ZZM;4ZZN;4ZZO;5AX3;5BUE;5BUI;5BUJ;5BVD;5BVE;5BVF;5K4I;5LCJ;5LCK;5NGU;5NHF;5NHH;5NHJ;5NHL;5NHO;5NHP;5NHV;5V60;5V61;5V62;5WP1;6D5Y;6DMG;6G54;6G8X;6G91;6G92;6G93;6G97;6G9A;6G9D;6G9H;6G9J;6G9K;6G9M;6G9N;6GDM;6GDQ;6GE0;6GJB;6GJD;6NBS;6OPG;6OPH;6OPI;6Q7K;6Q7S;6Q7T;6QA1;6QA3;6QA4;6QAG;6QAH;6QAL;6QAQ;6QAW;6RQ4;6SLG;7AUV;7E73;7E75;7NQQ;7NQW;7NR3;7NR5;7NR8;7NR9;7OPM;7W5O; |
 |
Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 389 |
| Target type | Protein | |
| Target unique ID | P28482 | |
| Activity | 2 nM | |
| Binding Conditions/Buffer | 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.05 μg/μl tRNA |
|
| Assay | N/A |
|
| PubMed ID | 12118241 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_382 | RNA | GGCGUGACCUGAUGAGUCACGCAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUGUGUUACGAAACGUUCCC | |
| Apta_522 | RNA | GGGCGACCCUGAUGAGCACACGAGGGGGAAACCCCGGACAAUCAGACACGGUGUUCGAAACGGUGAAAGCCGUAGGUUGCCCUUU | |
| Apta_612 | RNA | GGGCGACCCUGAUGAGCCUUAGGAUAUGCUUCGGCAGAAGGACGUCGAAACGGUGAAAGCCGUAGGUUGCCC | |
| Apta_1222 | RNA | GGAGGCGCCAACUGAAUGAAACCGUGCUAAGGAGUUGGACUCCGCUGCUUCGGCAGAGCGAUAUAGUGCCAGCUGAUACAUCCGUAACUAGUCGCGUCAC | |
| Apta_1223 | RNA | GGAGGCGCCAACUGAAUGAAAGGUUACGCUUCAACGCCGUGCAGCUGCUUCGGCAGGCUUGUGAGUACAUGGCCCAUUGAUCCGUAACUAGUCGCGUCAC | |
| Apta_1248 | RNA | GGGUGACUUUUCCCUCAGGCUCCUGUGAAGCAACCGAAUGGACUGAAAUGAAACCGCCC | |
| Apta_1224 | RNA | GGAGGCGCCAACUGAAUGAAUGUUCUGGAUCUGCUAGAGACUAUCUGCUUCGGCAGCACUCUCCGUUAACAACACGUACAUCCGUAACUAGUCGCGUCAC | |
| Apta_1225 | RNA | GGAGGCGCCAACUGAAUGAAAGAGGUAACGAAAGAUGAUUGUGCCUGCUUCGGCAGUACAACUUCUCGGAUAAAUGAUUAUCCGUAACUAGUCGCGUCAC | |
| Apta_6 | RNA | GGAAAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUCC | |
| Apta_155 | RNA | GACCCAGGGCCGGGACGCAAAGCAGCCAAAACAGACGGCCCCAGUCAGGGAGUU | |
| Apta_305 | RNA | GGGAGGACGAUGCGGAUCGCCCUGAACCGGCCCAGCAGACUGCUGACGGCACGAUCCGCAUCGUCCUCCC | |
| Apta_314 | RNA | GGUUACCAGCCUUCACUGCUCCCGGACGCGUCUGUAUCCGCUACCCGCCGCACCACGGUCGGUCACAC | |
| Apta_424 | RNA | UGCUAGUAUAUCGCACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG | |
| Apta_425 | RNA | UGCCGCCAUAUCACACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG | |
| Apta_453 | RNA | GGGCUAGCUGAUCGUACCAGUAGCGUGGCCUGGGGGGCCUAGUCGUGCGAUACUAACAGCUAACACCC | |
| Apta_1226 | RNA | GGAGGCGCCAACUGAAUGAAGGUGUAUUUCAGUUAAAGAACUUGCUGCUUCGGCAGCGUUCUGAGUUACUAGUCCAAUAAUCCGUAACUAGUCGCGUCAC | |
| Apta_723 | RNA | GGGAGCUCAGAAUAAACGCUCAAAUCCGUGGACAGGGCGUAAGCGCCUUCGACAUGAGACACGGAUCCUGCGACGAAUUCAGC | |
| Apta_376 | RNA | GGGAGCUCAGCCUUCACUGCACUCCGGCUGGUGGACGCGGUACGAGCAAUUUGUACCGGAUGGAUGUUCGGGCAGCGGUGUGGCAGGGAUGAGCGGCACCACGGUCGGAUCCAC | |
| Apta_854 | RNA | GGGAGCUCAGAAUAAACGCUCAAGGGUAGGGAUCGUUACCCCGACAUUUUAAUGGGCCGAUGUUUCGACAUGAGGCCCGGAUCCGGC | |
| Apta_598 | RNA | GGGAAUUCCGCGUGUGCGCCGCGGAAGACGUUGGAAGGAUAGAUACCUACAACGGGGAAUAUAGAGGCCAGCACAUAGUGAGGCCCUCCUCCC |
DEV TOOL
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