Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 419
Aptamer sequence: GUGAGACCAGCCGAGUGGUGUCUGGCUAUUCACUGGAGCGUGGGUGGAACCCCUGCGCACUCGUUUGGCUGUCCGGGCCUUCGGGCCGGGAUUAUCUCU
Target unique ID: P63244
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_413 | APC99 | Protein | RACK1_HUMAN | 137 nM | 10 mM sodium citrate, 150 mM KCl (pH 7.6) | 9546673 |
Structure information of aptamer
| Aptamer Sequence: | GUGAGACCAGCCGAGUGGUGUCUGGCUAUUCACUGGAGCGUGGGUGGAACCCCUGCGCACUCGUUUGGCUGUCCGGGCCUUCGGGCCGGGAUUAUCUCU |
| The optimal secondary structure in dot-bracket notation: | ..((((((((.((((((.(((..((((((((((......))))))))...))..))))))))).))))..((((.((((....)))).))))..)))). |
| The centroid secondary structure in dot-bracket notation: | ..((((((((.(((((..(((....((((((((......)))))))).......))).))))).))))..((((.((((....)))).))))..)))). |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_413 | Description | APC99 |
| Aptamer chemistry | RNA | Length | 99 nt |
| GC content | 62.6% | Molecular weight | 31,864.75 Da |
| Molarity of 1 μg/μl solution | 31.38 μM | Number of G-quadruplexes | 2 |
| G-Score | 19 | Function | Targeted binding |
| Sequence | GUGAGACCAGCCGAGUGGUGUCUGGCUAUUCACUGGAGCGUGGGUGGAACCCCUGCGCACUCGUUUGGCUGUCCGGGCCUUCGGGCCGGGAUUAUCUCU | ||
| Applications | Binding to human activated protein C (APC) | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P63244 | Protein name | Receptor of activated protein C kinase 1 (Cell proliferation-inducing gene 21 protein) (Guanine nucleotide-binding protein subunit beta-2-like 1) (Guanine nucleotide-binding protein subunit beta-like protein 12.3) (Human lung cancer oncogene 7 protein) (HLC-7) (Receptor for activated C kinase) (Small ribosomal subunit protein RACK1) [Cleaved into: Receptor of activated protein C kinase 1, N-terminally processed (Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed)] |
| Gene name(s) | RACK1 GNB2L1 HLC7 PIG21 | Organism | Receptor of activated protein C kinase 1 (Cell proliferation-inducing gene 21 protein) (Guanine nucleotide-binding protein subunit beta-2-like 1) (Guanine nucleotide-binding protein subunit beta-like protein 12.3) (Human lung cancer oncogene 7 protein) (HLC-7) (Receptor for activated C kinase) (Small ribosomal subunit protein RACK1) [Cleaved into: Receptor of activated protein C kinase 1, N-terminally processed (Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed)] |
| Status | reviewed in uniprot database | Length | 317 |
| Mass | 35,077 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | 4AOW;4UG0;4V6X;5A2Q;5AJ0;5FLX;5LKS;5OA3;5T2C;5VYC;6FEC;6G18;6G51;6G53;6G5H;6G5I;6IP5;6IP6;6IP8;6OLE;6OLF;6OLG;6OLI;6OLZ;6OM0;6OM7;6QZP;6XA1;6Y0G;6Y2L;6Y57;6YBS;6Z6L;6Z6M;6Z6N;6ZLW;6ZM7;6ZME;6ZMI;6ZMO;6ZMT;6ZMW;6ZN5;6ZOJ;6ZOL;6ZON;6ZP4;6ZUO;6ZV6;6ZVH;6ZVJ;6ZXD;6ZXE;6ZXF;6ZXG;6ZXH;7A09;7K5I; |
 |
Function | Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. |
Activity data
 |
Interaction ID | 419 |
| Target type | Protein | |
| Target unique ID | P63244 | |
| Activity | 137 nM | |
| Binding Conditions/Buffer | 10 mM sodium citrate, 150 mM KCl (pH 7.6) |
|
| Assay | Determination of Km values by Lineweaver-Burk plot of APC against Boc-Leu-Ser-Thr-Arg-NH-Mec as substrate with APC aptamers |
|
| PubMed ID | 9546673 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_353 | RNA | GAAUUCCGCGUGUGCCAGCGAAAGUUGCGUAUGGGUCACAUCGCAGGCACAUGUCAUCUGGGCGGUCCGUUCGGGAUCC | |
| Apta_1124 | RNA | GGGCGAAUUCGGGUUGGAUAGUAGGCGCAUAUGGCAUCUUCGUGGUUGUGUAUUGCCCUUUAGUGAGGGUUAAUU | |
| Apta_314 | RNA | GGUUACCAGCCUUCACUGCUCCCGGACGCGUCUGUAUCCGCUACCCGCCGCACCACGGUCGGUCACAC | |
| Apta_428 | RNA | GAAGCUUACAAGAAGGACAGCACGAAUAAAACCUGCGUAAAUCCGCCCCAUUUGUGUAAGGGUAGUGGGUCGAAUUCCGCUCA | |
| Apta_645 | RNA | CUGGACGACGGGGACGCCACUGGACUAGGUAAGCCAGGACCGUACGUCGGGAGCCGUCAGAAUA | |
| Apta_766 | RNA | GGUUACCAGCCUUCACUGCGGACGGACAGAGAGUGCAACCUGCCGUGCCGCACCACGGUCGGUCACAC | |
| Apta_886 | RNA | GGGAGACGAUAUUCGUCCAUUCGGGUGGCCCGUGUCUGAGCGGGGACGGCCACUUGAGCGCCGCUGUCCGACUGAAUUCUCGACC | |
| Apta_874 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUAGGAUAUGUCUGGAUACCAUGCAUGAUGCACCUUGGCAGUCUUACAGAAGGACGUCGAAACGGUAGCGAGAGCUC | |
| Apta_399 | RNA | GGGAGGACGAUGCGGCAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGGCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_499 | RNA | GGACGCGUGGUACCAGGCCGAUCUAUGGACGCUAUAGGCACACCGGAUACUUUAACGAUUGGCUAAGCUUCCGCGGGGAUC | |
| Apta_500 | RNA | GGGAGAAAGGGAAGCUUGAGGUGCUAUGGAGUGGAGGAGUUGAAGGUGUCGGGGUUGGCAGAAGAAGGCGAGCGUACGGAUCCAUC | |
| Apta_561 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUGUGGAAACAGACGUGGCACAUGACUACGUCGAAACGGUAGCGAGAGCUC | |
| Apta_710 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGUCUGGAUACCAUGCAUGAUGCACCUUGGCAGUCUUACGAAACGGUAGCGAGAGCUC | |
| Apta_894 | RNA | ACCGAGUCCAGAAGCUUGUAGUACUGACCCUUCCCGACGCACGGACCAGUGAUGCCAACCCACCCGCCUAGAUGGAGUUGAAUUCUCCCUAUAGUGAGUCGUAUUAC | |
| Apta_43 | RNA | GGGAGAGCGGAAGCGUGCUGGGCCACCAUCCGUAACUAGCUAAUACUUGUUAUCUUUUUAUUUUCAUAACCCAGAGGUCGAUGGAUCCCCCC | |
| Apta_276 | RNA | GGACCGAGAAGUUACCCUGUAAUCUUAGGAUGAAUCGCAUGCUCUAGCGACCUUUUCGGCUUCGGCGUACGCACAUCGCAGCAAC | |
| Apta_396 | RNA | GGGAGGACGAUGCGGCAGUCUGCAUCGUAGGAAUCGCCACCGUAUACUUUCCCACCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_414 | RNA | GGGAGGACGAUGCGGCCGAUUAGAACUGGGCUGAGGCGUUCUGCAUUUCGGUGAUCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_522 | RNA | GGGCGACCCUGAUGAGCACACGAGGGGGAAACCCCGGACAAUCAGACACGGUGUUCGAAACGGUGAAAGCCGUAGGUUGCCCUUU | |
| Apta_566 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCCUGCGAUGCAGAAAGGUGCUGACGACACAUCGAAACGGUAGCGAGAGCUC |
DEV TOOL
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