Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 894
Aptamer sequence: GGGTCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGG
Target unique ID: 43895
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_808 | long arm | Cell | Escherichia coli(E.coli)O157:H7 | 110 nM | 30 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol, and 1% BSA | 22166202 |
Structure information of aptamer
| Aptamer Sequence: | GGGTCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGG |
| The optimal secondary structure in dot-bracket notation: | ....((((((((((((.........)))).)))))))) |
| The centroid secondary structure in dot-bracket notation: | ....((((((((((((.........)))).)))))))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_808 | Description | long arm |
| Aptamer chemistry | DNA | Length | 38 nt |
| GC content | 52.6% | Molecular weight | 11,789.57 Da |
| Molarity of 1 μg/μl solution | 84.82 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Aptasensor |
| Sequence | GGGTCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGG | ||
| Applications | Diagnostic ligand against the pathogen-related food borne illness | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | 43895 | Cell name | Escherichia coli(E.coli) O157:H7 |
| Category | Bacteria | Organism | N/A |
| Product type | N/A | Cell type | N/A |
| Morphology | N/A | Tissue | N/A |
| Disease | N/A | Product format | Freeze-dried |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | Produces Shiga-like toxins I and II. Quality control strain for Difco products. |
|
|
| Application(s) | Media testing; Quality control | ||
Activity data
 |
Interaction ID | 894 |
| Target type | Cell | |
| Target unique ID | 43895 | |
| Activity | 110 nM | |
| Binding Conditions/Buffer | 30 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol, and 1% BSA |
|
| Assay | Comparison of binding ability of aptamer I-1 and its truncated aptamers to O157:H7 LPS versus K12 LPS |
|
| PubMed ID | 22166202 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_807 | DNA | GGGTCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGGTTACGTATTTGGTTTATAGATAGTAA | |
| Apta_806 | DNA | GGTTGGATGTAAGGTTGGAGGGGGG | |
| Apta_1045 | DNA | GGTGCTCAGGGAACTGTCTGAGGGATCAGGCTTAAGCCTGTCGAGCAGTT | |
| Apta_699 | DNA | ATCTAACTGCTGCGCCGCCGGGAAAATACTGTACGGTTAGA | |
| Apta_1062 | DNA | GGGGTCTTGCAGGTCCCGTAGGAGGGGCCATTGGAGTGGGG | |
| Apta_1280 | DNA | TCATATCCTTCTTGGTATCATTGCGTCATTTAGTCATTCATGTTATTCCCGAGGG | |
| Apta_163 | DNA | GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG | |
| Apta_503 | DNA | ACTTTTGAATGTGGCAACAAATTCGACAGG | |
| Apta_1193 | DNA | GGGTTGGAGGTGGAGGGGAGTTGAGG | |
| Apta_1256 | DNA | GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA | |
| Apta_57 | DNA | GCGGGTCGGTTGCTCGCTTCGCCCGATCGGTCTAAGGGTG | |
| Apta_267 | DNA | GAGTGCGTAATGGTACGATTTGGGAAGTGGCTTGGGGTGG | |
| Apta_911 | DNA | GGCCATTGTGGACTTCTTTGGGTAATTCAGGGGCTCGATT | |
| Apta_1018 | DNA | ATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGG | |
| Apta_735 | DNA | AGGCCCCCAGGCTCGGTGGATGCAAACACATGACTATGGGCCCGT | |
| Apta_58 | DNA | GCACCTTGATGACATGATAGTCGTTGTGTATGCAGTTGGC | |
| Apta_144 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG | |
| Apta_146 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG | |
| Apta_162 | DNA | CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG | |
| Apta_197 | DNA | GGCTTTAGGTCTGAGATCTCGGAAT |
DEV TOOL
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