Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 428
Aptamer sequence: GGGAGCTCAGAATAAACGCTCAATTCGGGAATGATTATCAAATTTATGCCCTCTGATTTCGACATGAGGCCCGGATC
Target unique ID: P9WIN9
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_422 M64CA Protein MP64_MYCTU N/A 20 mM HEPES, pH 7.35, 120 mM KCL, 1 mM CaCl2, and 1 mM MgCl2 19284297
Structure information of aptamer
Aptamer Sequence: GGGAGCTCAGAATAAACGCTCAATTCGGGAATGATTATCAAATTTATGCCCTCTGATTTCGACATGAGGCCCGGATC
The optimal secondary structure in dot-bracket notation: ..((((...........)))).(((((((....................((((((.......)).))))))))))).
The centroid secondary structure in dot-bracket notation: ..((((...........)))).(((((((....................((((............))))))))))).
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_422 Description M64CA
Aptamer chemistry DNA Length 77 nt
GC content 45.5% Molecular weight 23,754.25 Da
Molarity of 1 μg/μl solution 42.10 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Aptasensor
Sequence GGGAGCTCAGAATAAACGCTCAATTCGGGAATGATTATCAAATTTATGCCCTCTGATTTCGACATGAGGCCCGGATC
Applications Detecting M. tuberculosis
Target information
Type Detail Type Detail
Uniprot ID P9WIN9 Protein name Immunogenic protein MPT64 (Antigen MPT64)
Gene name(s) mpt64 Rv1980c MTCY39.39 Organism Immunogenic protein MPT64 (Antigen MPT64)
Status reviewed in uniprot database Length 228
Mass 24,855 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
2HHI;
Function N/A
Activity data
Interaction ID 428
Target type Protein
Target unique ID P9WIN9
Activity N/A
Binding Conditions/Buffer

20 mM HEPES, pH 7.35, 120 mM KCL, 1 mM CaCl2, and 1 mM MgCl2
Assay

Map of the sandwich assay to detect the 77 samples. (A) Non-tuberculosis Mycobacterium and BCG Danish; (B) M. tuberculosis including H37Rv and 50 isolations, and M. bovis
PubMed ID 19284297
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_501 DNA GGGAGACAAGAATAAACGCTCAATTTAACGTATTTATTCAAGCTCGTATTCGACAGGAGGCTCACAACAGGC 63.64%
Apta_502 DNA GGGAGACAAGAATAAACGCTCAAATGGACTTTTGAATGTGGCAACAAATTCGACAGGAGGCTCACAACAGGC 62.34%
Apta_490 DNA GGGAGACAAGAATAAACGCTCAAGGGCGTGGTGGGTGGGGTACTAATAATGTGCGTTTGTTCGACAGGAGGCTCACAACAGGC 61.45%
Apta_790 DNA GGGAGACAAGAATAAACGCTCAAGAAGTGAAAATGACAGAACACAACATTCGACAGGAGGCTCACAACAGGC 59.74%
Apta_854 RNA GGGAGCUCAGAAUAAACGCUCAAGGGUAGGGAUCGUUACCCCGACAUUUUAAUGGGCCGAUGUUUCGACAUGAGGCCCGGAUCCGGC 55.17%
Apta_177 DNA AGCAGCACAGAGGTCAGATGCCATAGGTGCCTGAATTGTCCCAAACATTCGCATGATCGACCTATGCGTGCTACCGTGAA 53.75%
Apta_232 DNA AGCAGCACAGAGGTCAGATGCTATGAGCAGATCCGATAGAAAGTTAATGCCCTAGAACATCCTATGCGTGCTACCGTGAA 53.75%
Apta_107 DNA AGCAGCACAGAGGTCAGATGTTCATTTCAGTTGACCGATAGCATTGAGCAATGATCTATTCCTATGCGTGCTACCGTGAA 52.50%
Apta_107 DNA AGCAGCACAGAGGTCAGATGTTCATTTCAGTTGACCGATAGCATTGAGCAATGATCTATTCCTATGCGTGCTACCGTGAA 52.50%
Apta_178 DNA AGCAGCACAGAGGTCAGATGCGGTATGCTGGGTCATCTAACTGGGGATATTCTGATATTGCCTATGCGTGCTACCGTGAA 52.50%
Apta_226 DNA GGGAGATCACTTACGGCACCGTATAGTCGTACATGAACGAGTGTGAGTGTTAAGCCTTTGGCGACAGGGCTCGGAACC 52.50%
Apta_272 RNA GGGAGACAAGAAUAAACGCUCAACACAGAACGCGGUCCCCACACAGGACAGGAGCCAGCCCCGGUUCGACAGGAGGCUCACAACAGGC 52.27%
Apta_433 RNA GGGAGACAAGAAUAAACGCUCAACGUUCAGUAUAACAGUCCGAGUCUAACACACAAUGGGACACUGAAUUCGACAGGAGGCUCACAACAGGC 52.17%
Apta_685 DNA GGGGCAACAAACACGGAAGAAAATGAATCTACATACGTGGACATATATCCTGCCGCGTG 51.95%
Apta_738 DNA ATCGTCTGCTCCGTCCAATACCTGCATATACACTTTGCATGTGGTTTGGTGTGAGGTCGTGC 51.95%
Apta_740 DNA ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC 51.95%
Apta_976 DNA GATGAATATCGCAATGAAATTATATATGTATAATTGGAACGGTGGTATCC 51.95%
Apta_1089 DNA ATCCAGAGTGACGCAGCAATTACCGATGGCTTGTTTGTAATGTAGGGTTCCGTCGGATTGGACACGGTGGCTTAGT 51.95%
Apta_401 DNA ATCCAGAGTGACGCAGCAGATAAGTAGGTATCCGTTTGAAAAACTTTTCTGACCGTCCGACTATGGACACGGTGGCTTAGT 51.85%
Apta_725 DNA ATCCAGAGTGACGCAGCATGCTCATCCACGATTCTGGCGAATTTAGTGCCTGTCTTTTTCTCTTGGACACGGTGGCTTAGT 51.85%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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