AptaDB: Result

Interaction ID: 1124

Aptamer sequence: CCGTATCGCCCAGGCAACTGGGCTAAACTTCCCAGAGGGAACGAAACCTGGG

Target unique ID: Q12797

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1070	AP-Cell 3	Protein	ASPH_HUMAN	65.23 nM	5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco’s PBS	34056254

Aptamer Sequence:	CCGTATCGCCCAGGCAACTGGGCTAAACTTCCCAGAGGGAACGAAACCTGGG
The optimal secondary structure in dot-bracket notation:	.......((((((....)))))).......((((..((........))))))
The centroid secondary structure in dot-bracket notation:	.......((((((....)))))).......((((..((........))))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1070	Description	AP-Cell 3
Aptamer chemistry	DNA	Length	52 nt
GC content	59.6%	Molecular weight	16,017.28 Da
Molarity of 1 μg/μl solution	62.43 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target detection
Sequence	CCGTATCGCCCAGGCAACTGGGCTAAACTTCCCAGAGGGAACGAAACCTGGG
Applications	Recognizing aspartate β?Hydroxylase

Type	Detail	Type	Detail
Uniprot ID	Q12797	Protein name	Aspartyl/asparaginyl beta-hydroxylase (EC 1.14.11.16) (Aspartate beta-hydroxylase) (ASP beta-hydroxylase) (Peptide-aspartate beta-dioxygenase)
Gene name(s)	ASPH BAH	Organism	Aspartyl/asparaginyl beta-hydroxylase (EC 1.14.11.16) (Aspartate beta-hydroxylase) (ASP beta-hydroxylase) (Peptide-aspartate beta-dioxygenase)
Status	reviewed in uniprot database	Length	758
Mass	85,863	Activity regulation	N/A
Involvement in disease	Facial dysmorphism, lens dislocation, anterior segment abnormalities, and spontaneous filtering blebs (FDLAB)	Catalytic activity	Reaction=2-oxoglutarate + L-aspartyl-[protein] + O2 = 3-hydroxy-L-aspartyl-[protein] + CO2 + succinate
PDB ID(s)	5APA;5JQY;5JTC;5JZ6;5JZ8;5JZA;5JZU;6Q9F;6Q9I;6QA5;6RK9;6YYU;6YYV;6YYW;6YYX;6YYY;6Z6Q;6Z6R;7BMI;7BMJ;7E6J;
Function	Specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.

	Interaction ID	1124
	Target type	Protein
	Target unique ID	Q12797
	Activity	65.23 nM
	Binding Conditions/Buffer	5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco’s PBS
	Assay	Affinity assay of selected aptamers using flow cytometry
	PubMed ID	34056254

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_336	DNA	CATGCTTCCCCAGGGAGATGACCGGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTGG	54.17%
Apta_3	DNA	CCTGCCTTTGGCAGCCGGGCGAAGGCAACCCGACACCGACAGC	51.92%
Apta_421	DNA	ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA	51.92%
Apta_835	DNA	TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC	51.92%
Apta_861	DNA	CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG	51.92%
Apta_873	DNA	CCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTG	51.92%
Apta_875	DNA	GCCGCGACCATACAACGCAAACAACACGCCTGTACGCTTG	51.92%
Apta_600	DNA	GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC	51.72%
Apta_600	DNA	GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC	51.72%
Apta_888	DNA	GGCGACCCCCGGGCTACCAGACAATGTACGCAGCAAGAGTGACGGTCGTACCTCGGAGTC	51.67%
Apta_1035	DNA	ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC	51.67%
Apta_1117	DNA	CGTACGGAATTCGCTAGCGGACTGGAGTCTAGACCGGGTAGCTGTGGTGGATCCGAGCTCCACGTG	51.52%
Apta_990	DNA	AACCCAACCCCGCACCATTAGTCTAAATTTTCGCGACGGAACACAGCAACAACTCCCCA	50.85%
Apta_76	DNA	CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG	50.00%
Apta_181	DNA	CCGTGTAAGGTTTCAGTGGCTTATCCGAAGTAACCCGAGA	50.00%
Apta_261	DNA	TAAACCCCAAAACAGTGCAACTAGGTGTAGGTCCCGTGGT	50.00%
Apta_462	DNA	GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA	50.00%
Apta_469	DNA	CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAG	50.00%
Apta_470	DNA	CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCTCAT	50.00%
Apta_473	DNA	CCCTGCACTCGCAATGGCCCCTCTACACCACAAACGGACA	50.00%

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