Aptamer-target interaction descriptor
Aptamer-target interaction information
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_390 | T-SO530 | Protein | SYUA_HUMAN | 63 nM | 10 mM Tris-HCl, 150 mM NaCl, 5 mM KCl, pH 7.4 with 0.05% Tween 20 | 22697251 |
Structure information of aptamer
| Aptamer Sequence: | GGTGCGGCGGGACTAGTGGGTGTG |
| The optimal secondary structure in dot-bracket notation: | (((........))).......... |
| The centroid secondary structure in dot-bracket notation: | ........................ |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_390 | Description | T-SO530 |
| Aptamer chemistry | DNA | Length | 24 nt |
| GC content | 70.8% | Molecular weight | 7,561.84 Da |
| Molarity of 1 μg/μl solution | 132.24 μM | Number of G-quadruplexes | 2 |
| G-Score | 18 | Function | Targeted binding |
| Sequence | GGTGCGGCGGGACTAGTGGGTGTG | ||
| Applications | Development of drugs and diagnostic tests for neurodegenerative diseases | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P37840 | Protein name | Alpha-synuclein (Non-A beta component of AD amyloid) (Non-A4 component of amyloid precursor) (NACP) |
| Gene name(s) | SNCA NACP PARK1 | Organism | Alpha-synuclein (Non-A beta component of AD amyloid) (Non-A4 component of amyloid precursor) (NACP) |
| Status | reviewed in uniprot database | Length | 140 |
| Mass | 14,460 | Activity regulation | N/A |
| Involvement in disease | Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies) | Catalytic activity | N/A |
| PDB ID(s) | 1XQ8;2JN5;2KKW;2M55;2N0A;2X6M;3Q25;3Q26;3Q27;3Q28;3Q29;4BXL;4R0U;4R0W;4RIK;4RIL;4ZNN;5CRW;6A6B;6CT7;6CU7;6CU8;6FLT;6H6B;6I42;6L1T;6L1U;6L4S;6LRQ;6OSJ;6OSL;6OSM;6PEO;6PES;6RT0;6RTB;6SST;6SSX;6UFR;6XYO;6XYP;6XYQ;7C1D;7E0F;7L7H;7LC9;7NCA;7NCG;7NCH;7NCI;7NCJ;7NCK;7STX; |
 |
Function | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. |
Activity data
 |
Interaction ID | 396 |
| Target type | Protein | |
| Target unique ID | P37840 | |
| Activity | 63 nM | |
| Binding Conditions/Buffer | 10 mM Tris-HCl, 150 mM NaCl, 5 mM KCl, pH 7.4 with 0.05% Tween 20 |
|
| Assay | ELONA analysis of the binding of FITC-labeled aptamers to Aβ1?40 oligomer. A 0.43 μg sample of Aβ1?40 oligomer was immobilized in a 96-well microtiter plate |
|
| PubMed ID | 22697251 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_483 | DNA | ATTGGGAGGGATTGGGTGGG | |
| Apta_14 | DNA | AGCGGGGGGGGCTGGGCTGGGTGGG | |
| Apta_22 | DNA | TGTGGGGGTGGACTGGGTGGGTACC | |
| Apta_757 | DNA | GGTGGTGGTGGTTGTGGTGGTGG | |
| Apta_350 | DNA | GGTGGTGGTGGTTGTGGTGGTGGTGG | |
| Apta_10 | DNA | GGGGGGGGTGGCTGGGCGGGGTGGG | |
| Apta_13 | DNA | AGGGGGGGGGGAGGGGTCGGGGTGG | |
| Apta_16 | DNA | AGGGGGGGGGGACGGGACGGGTATA | |
| Apta_21 | DNA | TGTGGGGGAGGACGGGCGGGGATGC | |
| Apta_124 | DNA | GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC | |
| Apta_683 | DNA | GCAGTACGATCGGGGGTGGGTGGATGTAGG | |
| Apta_141 | DNA | GTTGACGGGCGATTGGTCTT | |
| Apta_693 | DNA | CGAGTTGAGCCGGGCGCGGTACGGGTACTGGTATGTGTGG | |
| Apta_1259 | DNA | GGCGCGGCATGGTGTGGACTCCAGGCGGTAGGGATGTCGT | |
| Apta_245 | DNA | ACAGGGGTGTGGGGACAGGGGTGTGGGG | |
| Apta_675 | DNA | ACTGTCCGTCGGGTTTAGGGTGGCGTTCGG | |
| Apta_679 | DNA | CAGGTGGCAGATCGGGTGTGGGTGGCCTGG | |
| Apta_1236 | DNA | GGACTGTTGTGGTATTATTTTTGGTTGTGC | |
| Apta_1237 | DNA | GGGGTTGTGTGGGGAGTTTGTCCTGTTGTG | |
| Apta_1239 | DNA | GGTGGATGGGGATGCTTTGTTAGTTTGTGC |
DEV TOOL
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