Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 886
Aptamer sequence: ATGAGAAGCGTCGGTGTGGTTAAACACGGAACGAAGGTGCAGGAAGATTTGTCGATGCGGTGCCTGAGCGGGCTGGCAAGGCGCATA
Target unique ID: CL-173
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_802 | Adipo-8 | Cell | Mature 3T3-L1 adipocytes (adipose tissue) | 17.8 nM | 4.5 mg/ml glucose and 5 mM MgCl2 in D-PBS with calcium chloride and magnesium chloride (Sigma), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml). pH 7.1-7.5. 4°C | 22662223 |
Structure information of aptamer
| Aptamer Sequence: | ATGAGAAGCGTCGGTGTGGTTAAACACGGAACGAAGGTGCAGGAAGATTTGTCGATGCGGTGCCTGAGCGGGCTGGCAAGGCGCATA |
| The optimal secondary structure in dot-bracket notation: | .......((((((((((......))))...((....))((((......)))))))))).((((((..((......)).))))))... |
| The centroid secondary structure in dot-bracket notation: | .......((((((((((......))))...........((((......)))))))))).((((((..((......)).))))))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_802 | Description | Adipo-8 |
| Aptamer chemistry | DNA | Length | 87 nt |
| GC content | 56.3% | Molecular weight | 27,226.41 Da |
| Molarity of 1 μg/μl solution | 36.73 μM | Number of G-quadruplexes | 2 |
| G-Score | 18 | Function | Target recognition |
| Sequence | ATGAGAAGCGTCGGTGTGGTTAAACACGGAACGAAGGTGCAGGAAGATTTGTCGATGCGGTGCCTGAGCGGGCTGGCAAGGCGCATA | ||
| Applications | Molecular recognition tool | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CL-173 | Cell name | Mature 3T3-L1 adipocytes (adipose tissue) |
| Category | Animal cells | Organism | Mus musculus, mouse |
| Product type | N/A | Cell type | fibroblast |
| Morphology | fibroblast | Tissue | Embryo |
| Disease | N/A | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | Triglycerides |
| Cell description | 3T3-L1 is a fibroblast that was isolated from the embryo of a mouse. This cell line can be used to study the basic cellular mechanisms associated with diabetes, obesity, and related disorders. |
|
|
| Application(s) | 3D cell culture | ||
Activity data
 |
Interaction ID | 886 |
| Target type | Cell | |
| Target unique ID | CL-173 | |
| Activity | 17.8 nM | |
| Binding Conditions/Buffer | 4.5 mg/ml glucose and 5 mM MgCl2 in D-PBS with calcium chloride and magnesium chloride (Sigma), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml). pH 7.1-7.5. 4°C |
|
| Assay | Kd determination for adipo8 |
|
| PubMed ID | 22662223 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_803 | DNA | ATGAGAAGCGTCGGTGTGGTTACTCCGGGCACTTGATATATCGATATGGAGAATAATGCACCCTGAGCGGGCTGGCAAGGCGCATA | |
| Apta_1175 | DNA | ATGAGAGCGTCGGTGTGGTATCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGAGGGTGCGGAAGTA | |
| Apta_1174 | DNA | ATGAGAGCGTCGGTGTGGTAAGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACTCTTGCTGTAGGAGGGTGCGGAAGTA | |
| Apta_235 | DNA | AGCAGCACAGAGGTCAGATGTGGTTCGATGCTGCAAGGTGTTGTCATTTGGCATATGTTACCTATGCGTGCTACCGTGAA | |
| Apta_61 | DNA | AGCAGCACAGAGGTCAGATGGCAGGTAAGCAGGCGGCTCACAAAACCATTCGCATGCGGCCCTATGCGTGCTACCGTGAA | |
| Apta_110 | DNA | AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA | |
| Apta_110 | DNA | AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA | |
| Apta_173 | DNA | AGCAGCACAGAGGTCAGATGGCAGGTTTCGACGTACAATGCTATGGAGGCTTTATGATCGCCTATGCGTGCTACCGTGAA | |
| Apta_451 | DNA | ATACCAGCTTATTCAATTGAGTAAGAGTGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCAATCT | |
| Apta_731 | DNA | AAGGAGCAGCGTGGAGGATACTCTAATTGCCGTATAAGGTCAGGGGGTTGGTTGGTTCCTAGTGCTTAGGGTGTGTCGTCGTGGT | |
| Apta_836 | DNA | TGAGCCCAAGCCCTGGTATGTTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGGGGCAGGTCTACTTTGGGATC | |
| Apta_898 | DNA | ATACGGGAGCCAACACCAACAAAACTAGCAAATTAGAGCCGTATCGGAGCCTATAGAGCAGGTGTGACGGAT | |
| Apta_1082 | DNA | GGCTGGTCCGCTGGGAACAAGGGCGGGAGGGAGGGTGTGGGTGCGACAAGCGGACCAGCC | |
| Apta_1031 | DNA | GGAGACCGCTTAAGCTTAAAGGAGCGGTTACGAATGCGATGACTTAGGTACCATTGCACTACGGTACCTAAACAGTTCCTTTGGATCCGAATTCGCCGC | |
| Apta_625 | DNA | ATACCAGCTTATTCAATTCAGGGCGGTATGAGGCTCGATCAAGGTCGGAGCGAGAATTTTTTCGCGGAGTCGGCTGGATCAGATAGTAAGTGCAATCT | |
| Apta_625 | DNA | ATACCAGCTTATTCAATTCAGGGCGGTATGAGGCTCGATCAAGGTCGGAGCGAGAATTTTTTCGCGGAGTCGGCTGGATCAGATAGTAAGTGCAATCT | |
| Apta_78 | DNA | ATCTCGACTAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACAATACTCGT | |
| Apta_106 | DNA | AGCAGCACAGAGGTCAGATGGAGCCAGGAGTCGGCCTACAGAAGATTCTGATGTACCTCACCTATGCGTGCTACCGTGAA | |
| Apta_106 | DNA | AGCAGCACAGAGGTCAGATGGAGCCAGGAGTCGGCCTACAGAAGATTCTGATGTACCTCACCTATGCGTGCTACCGTGAA | |
| Apta_178 | DNA | AGCAGCACAGAGGTCAGATGCGGTATGCTGGGTCATCTAACTGGGGATATTCTGATATTGCCTATGCGTGCTACCGTGAA |
DEV TOOL
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