Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 874
Aptamer sequence: GGGAGACAAGAATAAACGCTCAAGAAGTGAAAATGACAGAACACAACATTCGACAGGAGGCTCACAACAGGC
Target unique ID: HTB-22
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_790 MUC1-5TR-1 Cell Human breast adenocarcinoma (MCF7) cells 47.3 nM 0.5 M NaCl, 10 mM Tris-HCl, 1 mM MgCl2, in Nuclease Free Water, pH 7.5-7.6 22861487
Structure information of aptamer
Aptamer Sequence: GGGAGACAAGAATAAACGCTCAAGAAGTGAAAATGACAGAACACAACATTCGACAGGAGGCTCACAACAGGC
The optimal secondary structure in dot-bracket notation: ..................(((..(((((.................)).))).....))).............
The centroid secondary structure in dot-bracket notation: ........................................................................
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_790 Description MUC1-5TR-1
Aptamer chemistry DNA Length 72 nt
GC content 45.8% Molecular weight 22,353.44 Da
Molarity of 1 μg/μl solution 44.74 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted therapy
Sequence GGGAGACAAGAATAAACGCTCAAGAAGTGAAAATGACAGAACACAACATTCGACAGGAGGCTCACAACAGGC
Applications Targeted therapy of MCF7 adenocarcinoma cell
Target information
Type Detail Type Detail
ATCC ID HTB-22 Cell name Human breast adenocarcinoma (MCF7) cells
Category Human cells Organism Homo sapiens, human
Product type N/A Cell type epithelial cell
Morphology epithelial Tissue Breast; Mammary gland
Disease Adenocarcinoma Product format Frozen
Antigen expression Blood Type O; Rh+ Genes expressed Insulin-like growth factor binding protein (IGFBP) BP-2; insulin-like growth factor binding protein (IGFBP) BP-4; insulin-like growth factor binding protein (IGFBP) BP-5
Cell description MCF7 are epithelial cells isolated from the breast tissue of a 69-year-old, White, female patient with metastatic adenocarcinoma. Use these cells in your breast cancer research.
Application(s) 3D cell culture; Immuno-oncology
Activity data

No relevant experimental diagram

 Interaction ID 874
Target type Cell
Target unique ID HTB-22
Activity 47.3 nM
Binding Conditions/Buffer

0.5 M NaCl, 10 mM Tris-HCl, 1 mM MgCl2, in Nuclease Free Water, pH 7.5-7.6
Assay

N/A
PubMed ID 22861487
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_502 DNA GGGAGACAAGAATAAACGCTCAAATGGACTTTTGAATGTGGCAACAAATTCGACAGGAGGCTCACAACAGGC
80.56%
Apta_501 DNA GGGAGACAAGAATAAACGCTCAATTTAACGTATTTATTCAAGCTCGTATTCGACAGGAGGCTCACAACAGGC 77.78%
Apta_490 DNA GGGAGACAAGAATAAACGCTCAAGGGCGTGGTGGGTGGGGTACTAATAATGTGCGTTTGTTCGACAGGAGGCTCACAACAGGC 71.08%
Apta_272 RNA GGGAGACAAGAAUAAACGCUCAACACAGAACGCGGUCCCCACACAGGACAGGAGCCAGCCCCGGUUCGACAGGAGGCUCACAACAGGC 68.18%
Apta_433 RNA GGGAGACAAGAAUAAACGCUCAACGUUCAGUAUAACAGUCCGAGUCUAACACACAAUGGGACACUGAAUUCGACAGGAGGCUCACAACAGGC 65.22%
Apta_137 RNA GGGAGACAAGAAUAAACGCUCAAGUGACGUCCUGAUCGAUUGUGCAUUCGGUGUGACGAUCUUUCGACAGGAGGCUCACAACAGGC 65.12%
Apta_432 RNA GGGAGACAAGAAUAAACGCUCAAGGCAUUUCUGCUAGUCUGGUUGUAAGAUAUUCAACACGUGAGUUUCGACAGGAGGCUCACAACAGGC 63.33%
Apta_422 DNA GGGAGCTCAGAATAAACGCTCAATTCGGGAATGATTATCAAATTTATGCCCTCTGATTTCGACATGAGGCCCGGATC 59.74%
Apta_723 RNA GGGAGCUCAGAAUAAACGCUCAAAUCCGUGGACAGGGCGUAAGCGCCUUCGACAUGAGACACGGAUCCUGCGACGAAUUCAGC 56.63%
Apta_171 RNA GGGAGCUCAGAAUAAACGCUCAACCAGUCUUGUGGCUUUGAAAGAGAGGAGUGUUCGACAUGAGGCCCGGAUCCGGC 55.84%
Apta_1068 DNA CGGGACAAGACAACGAACGAACAGGAAGAGAACCGGAATGCAGACGTCAGGG 55.56%
Apta_854 RNA GGGAGCUCAGAAUAAACGCUCAAGGGUAGGGAUCGUUACCCCGACAUUUUAAUGGGCCGAUGUUUCGACAUGAGGCCCGGAUCCGGC 55.17%
Apta_316 DNA CCGCCCAAATCCCTAAGAGAAGACTGTAATGACATCAAACCAGACACACTACACACGCA 54.17%
Apta_684 DNA GGCGGACCAAGGGGACACCACAGATGAATGTACAGTACCATGTTACTGCGCCCGTAGGTG 54.17%
Apta_685 DNA GGGGCAACAAACACGGAAGAAAATGAATCTACATACGTGGACATATATCCTGCCGCGTG 54.17%
Apta_1113 DNA CCGCCCAAATCCCTAAGAGCACAAACACCAAACACAACCACCCCAACCAGACACACTACACACGCA 54.17%
Apta_948 DNA TAGGGAAGAGAAGGACATATGATACTGGCCTTGACACCCTGTTGTGGCTTGATGACAATAACATTGACTAGTACATGACCACTTGA 53.49%
Apta_903 DNA ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGATACTCTATTCGAGAGCAGGTGTGACGGAT 52.78%
Apta_904 DNA ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGAGTACTCTATTCGAGAGCAGGTGTGACGGAT 52.78%
Apta_1048 DNA ATACGGGAGCCAACACCACCTGCGGAAAATCAACAACGACACACGCCACGATTCAGAGCAGGTGTGACGGAT 52.78%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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