Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 353
Aptamer sequence: ACTTGCTCTGATGCGACAACCTTTTGCCCAGATAGTAAGT
Target unique ID: P19022
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_348 | NS13 | Protein | CADH2_HUMAN | 98 nM | 1X PBS | 33146506 |
Structure information of aptamer
| Aptamer Sequence: | ACTTGCTCTGATGCGACAACCTTTTGCCCAGATAGTAAGT |
| The optimal secondary structure in dot-bracket notation: | ((((((((((..((((.......)))).))))..)))))) |
| The centroid secondary structure in dot-bracket notation: | ((((((((((..((((.......)))).))))..)))))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_348 | Description | NS13 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 45.0% | Molecular weight | 12,485.83 Da |
| Molarity of 1 μg/μl solution | 80.09 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target detection |
| Sequence | ACTTGCTCTGATGCGACAACCTTTTGCCCAGATAGTAAGT | ||
| Applications | Detection of N-cadherin protein | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P19022 | Protein name | Cadherin-2 (CDw325) (Neural cadherin) (N-cadherin) (CD antigen CD325) |
| Gene name(s) | CDH2 CDHN NCAD | Organism | Cadherin-2 (CDw325) (Neural cadherin) (N-cadherin) (CD antigen CD325) |
| Status | reviewed in uniprot database | Length | 906 |
| Mass | 99,809 | Activity regulation | N/A |
| Involvement in disease | Arrhythmogenic right ventricular dysplasia, familial, 14 (ARVD14) | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. |
Activity data
 |
Interaction ID | 353 |
| Target type | Protein | |
| Target unique ID | P19022 | |
| Activity | 98 nM | |
| Binding Conditions/Buffer | 1X PBS |
|
| Assay | Dissociation constant (Kd) curve of NS13 and NC23 for N-cadherin. The standard deviations were obtained from three separate trials |
|
| PubMed ID | 33146506 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_694 | DNA | ACTCACTATGGAAGAGATGGCGACATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT | |
| Apta_449 | DNA | CGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCG | |
| Apta_1162 | DNA | AGAGGCTACAGCGATAAGTCGACATTGCTGACCGTACCTAGTAATACGT | |
| Apta_503 | DNA | ACTTTTGAATGTGGCAACAAATTCGACAGG | |
| Apta_1061 | DNA | TATTTATTGAATGCTGTAATTTCGCCACGCGAAAGTAGG | |
| Apta_1065 | DNA | CCTCGCTATGATGGAGTGCGTTTAGATCAGGGAACGGGTT | |
| Apta_930 | DNA | TTGCACTATGTTTTAGCTAGGGTTCCCTCCGGAGATAGTAAGTGCAA | |
| Apta_621 | DNA | AAGCTTGCTTTATAGCCTGCAGCGATTCTTGATCGGAAAAGGCTGAGAGCTACGC | |
| Apta_250 | DNA | CCCTGGCCGCCCTCTGGCGCGCTGATGT | |
| Apta_302 | DNA | ATCCTGTGAGGAATGCTCATGCATAGCAAGGGCT | |
| Apta_463 | DNA | GTTCTCATGCACACTTATAGCGGGGGGATCCAGTACT | |
| Apta_692 | DNA | ACGTTGACGCTGGTGCCCGCTTGTGGTGCGAGTGTTGTGT | |
| Apta_1208 | DNA | CCCTGCGGGGCTGCCCGATATGTGTCCAGGTGGTG | |
| Apta_1282 | DNA | TCCTCTCACACATCTTATCCCCTAGGG | |
| Apta_340 | DNA | TACTCAGGGCACTGCAAGCAATTGTGGTCCCAATGGGCTGAGTA | |
| Apta_238 | DNA | CACACATTCCCGTCCTCGATACGTCTAGGCTTAGTGCCACTTGCTTAATC | |
| Apta_514 | DNA | ACTCAGCACGCGTGTAGGGAAAACCAACCGCCCACAAGCTGCATGCAACG | |
| Apta_622 | DNA | AAGCTTTTTTGACTGACTGCAGCGATTCTTGATCGCCACGGTCTGGAAAAAGAG | |
| Apta_821 | DNA | CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC | |
| Apta_910 | DNA | TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA |
DEV TOOL
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