Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 414
Aptamer sequence: GCAATGGTACGGTACTTCCGTCATCAGCTTGTGATGTGGATGCGAACTGCAAAAGTGCACGCTACTTTGCTAA
Target unique ID: P56817
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_408 A1 Protein BACE1_HUMAN 68.6 nM 5 M guanidine-HCl in 50 mM Tris 31513457
Structure information of aptamer
Aptamer Sequence: GCAATGGTACGGTACTTCCGTCATCAGCTTGTGATGTGGATGCGAACTGCAAAAGTGCACGCTACTTTGCTAA
The optimal secondary structure in dot-bracket notation: ((((.(((((((((((((((.(((((.....)))))))))(((.....)))..))))).)).))))))))...
The centroid secondary structure in dot-bracket notation: ((((.(((((((((((((((.(((((.....)))))))))(((.....)))..))))).)).))))))))...
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_408 Description A1
Aptamer chemistry DNA Length 73 nt
GC content 47.9% Molecular weight 22,541.44 Da
Molarity of 1 μg/μl solution 44.36 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted therapy
Sequence GCAATGGTACGGTACTTCCGTCATCAGCTTGTGATGTGGATGCGAACTGCAAAAGTGCACGCTACTTTGCTAA
Applications Aptamer A1 is a novel specific and potent BACE1 inhibitor and is a promising potential target for the treatment of AD
Target information
Type Detail Type Detail
Uniprot ID P56817 Protein name Beta-secretase 1 (EC 3.4.23.46) (Aspartyl protease 2) (ASP2) (Asp 2) (Beta-site amyloid precursor protein cleaving enzyme 1) (Beta-site APP cleaving enzyme 1) (Memapsin-2) (Membrane-associated aspartic protease 2)
Gene name(s) BACE1 BACE KIAA1149 Organism Beta-secretase 1 (EC 3.4.23.46) (Aspartyl protease 2) (ASP2) (Asp 2) (Beta-site amyloid precursor protein cleaving enzyme 1) (Beta-site APP cleaving enzyme 1) (Memapsin-2) (Membrane-associated aspartic protease 2)
Status reviewed in uniprot database Length 501
Mass 55,764 Activity regulation Inhibited by RTN3 and RTN4
Involvement in disease N/A Catalytic activity Reaction=Broad endopeptidase specificity
PDB ID(s)
1FKN;1M4H;1PY1;1SGZ;1TQF;1UJJ;1UJK;1W50;1W51;1XN2;1XN3;1XS7;1YM2;1YM4;2B8L;2B8V;2F3E;2F3F;2FDP;2G94;2HIZ;2HM1;2IQG;2IRZ;2IS0;2NTR;2OAH;2OF0;2OHK;2OHL;2OHM;2OHN;2OHP;2OHQ;2OHR;2OHS;2OHT;2OHU;2P4J;2P83;2P8H;2PH6;2PH8;2Q11;2Q15;2QK5;2QMD;2QMF;2QMG;2QP8;2QU2;2QU3;2QZK;2QZL;2VA5;2VA6;2VA7;2VIE;2VIJ;2VIY;2VIZ;2VJ6;2VJ7;2VJ9;2VKM;2VNM;2VNN;2WEZ;2WF0;2WF1;2WF2;2WF3;2WF4;2WJO;2XFI;2XFJ;2XFK;2ZDZ;2ZE1;2ZHR;2ZHS;2ZHT;2ZHU;2ZHV;2ZJH;2ZJI;2ZJJ;2ZJK;2ZJL;2ZJM;2ZJN;3BRA;3BUF;3BUG;3BUH;3CIB;3CIC;3CID;3CKP;3CKR;3DM6;3DUY;3DV1;3DV5;3EXO;3FKT;3H0B;3HVG;3HW1;3I25;3IGB;3IN3;3IN4;3IND;3INE;3INF;3INH;3IVH;3IVI;3IXJ;3IXK;3K5C;3K5D;3K5F;3K5G;3KMX;3KMY;3KN0;3KYR;3L38;3L3A;3L58;3L59;3L5B;3L5C;3L5D;3L5E;3L5F;3LHG;3LNK;3LPI;3LPJ;3LPK;3MSJ;3MSK;3MSL;3N4L;3NSH;3OHF;3OHH;3OOZ;3PI5;3QBH;3QI1;3R1G;3R2F;3RSV;3RSX;3RTH;3RTM;3RTN;3RU1;3RVI;3S2O;3S7L;3S7M;3SKF;3SKG;3TPJ;3TPL;3TPP;3TPR;3U6A;3UDH;3UDJ;3UDK;3UDM;3UDN;3UDP;3UDQ;3UDR;3UDY;3UFL;3UQP;3UQR;3UQU;3UQW;3UQX;3VEU;3VF3;3VG1;3VV6;3VV7;3VV8;3WB4;3WB5;3ZMG;3ZOV;4ACU;4ACX;4AZY;4B00;4B05;4B0Q;4B1C;4B1D;4B1E;4B70;4B72;4B77;4B78;4BEK;4BFD;4D83;4D85;4D88;4D89;4D8C;4DH6;4DI2;4DJU;4DJV;4DJW;4DJX;4DJY;4DPF;4DPI;4DUS;4DV9;4DVF;4EWO;4EXG;4FCO;4FGX;4FM7;4FM8;4FRI;4FRJ;4FRK;4FRS;4FS4;4FSE;4FSL;4GID;4GMI;4H1E;4H3F;4H3G;4H3I;4H3J;4HA5;4HZT;4I0D;4I0E;4I0F;4I0G;4I0H;4I0I;4I0J;4I0Z;4I10;4I11;4I12;4I1C;4IVS;4IVT;4J0P;4J0T;4J0V;4J0Y;4J0Z;4J17;4J1C;4J1E;4J1F;4J1H;4J1I;4J1K;4JOO;4JP9;4JPC;4JPE;4K8S;4K9H;4KE0;4KE1;4L7G;4L7H;4L7J;4LC7;4LXA;4LXK;4LXM;4N00;4PZW;4PZX;4R5N;4R8Y;4R91;4R92;4R93;4R95;4RCD;4RCE;4RCF;4RRN;4RRO;4RRS;4TRW;4TRY;4TRZ;4WTU;4WY1;4WY6;4X2L;4X7I;4XKX;4XXS;4YBI;4ZPE;4ZPF;4ZPG;4ZSM;4ZSP;4ZSQ;4ZSR;5CLM;5DQC;5ENK;5ENM;5EZX;5EZZ;5F00;5F01;5HD0;5HDU;5HDV;5HDX;5HDZ;5HE4;5HE5;5HE7;5HTZ;5HU0;5HU1;5I3V;5I3W;5I3X;5I3Y;5IE1;5KQF;5KR8;5MBW;5MCO;5MCQ;5MXD;5QCO;5QCP;5QCQ;5QCR;5QCS;5QCT;5QCU;5QCV;5QCW;5QCX;5QCY;5QCZ;5QD0;5QD1;5QD2;5QD3;5QD4;5QD5;5QD6;5QD7;5QD8;5QD9;5QDA;5QDB;5QDC;5QDD;5T1U;5T1W;5TOL;5UYU;5V0N;5YGX;5YGY;6BFD;6BFE;6BFW;6BFX;6C2I;6DHC;6DMI;6E3Z;6EJ2;6EJ3;6EQM;6FGY;6JSE;6JSF;6JSG;6JSN;6JT3;6JT4;6NV7;6NV9;6NW3;6OD6;6PZ4;6UVP;6UVV;6UVY;6UWP;6UWV;6WNY;7B1E;7B1P;7B1Q;7D2V;7D2X;7D36;7D5A;7DCZ;7F1D;7MYI;7MYR;7MYU;7N66;
Function Responsible for the proteolytic processing of the amyloid precursor protein (APP).
Activity data
Interaction ID 414
Target type Protein
Target unique ID P56817
Activity 68.6 nM
Binding Conditions/Buffer

5 M guanidine-HCl in 50 mM Tris
Assay

Chronic intracerebroventricular injections of aptamer A1 inhibit the protein expression of BACE1 and sAPPb. Western blot detection of the protein expression levels of BACE1 in hippocampal homogenates (n = 4 per group). The data are presented as the mean – SEM. P < 0.01 relative to the A1-scr treated Tg6799 mice. sAPPb, soluble amyloid precursor protein b
PubMed ID 31513457
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_994 DNA GCAATGGTACGGTACTTCCGGACAGTGCTGAAAACTGTGACCCCCCAAAAGTGCACGCTACTTTGCTAA 76.71%
Apta_409 DNA GCAATGGTACGGTACTTCCGGATGCGGAAACTGGCTAATTGGTGAGGCTGGGGCGGTCGTGCAGCAAAAGTGCACGCTACTTTGCTAA 73.86%
Apta_995 DNA GCAATGGTACGGTACTTCCCCACCCCACGCTGCTCCCAAAAGTGCACGCTACTTTGCTAA 72.60%
Apta_151 DNA GCAATGGTACGGTACTTCCGCGGTCCGGGGTGGGTGGGTGGTGGGGGGTGCGGGGGGGCGGCCGCAAAAGTGCACGCTACTTTGCTAA 70.45%
Apta_81 RNA GCAAUGGUACGGUACUUCCAAGUGCGGGGGGGAGGUGGUGGUUCCAAAAGUGCACGCUACUUUGCUAA 58.90%
Apta_80 RNA GCAAUGGUACGGUACUUCCUUUGGAAGAUAGCUGGAGAACUAACCAAAAGUGCACGCUACUUUGCUAA 57.53%
Apta_667 DNA GCGGCGGTACCAGGTCAAAGGTGGGTGAGGGGACGCCAAGAGTCCCCGCGGTTACATAGAG 54.79%
Apta_1277 DNA TCCGTGGTACGGTTACTCGTTCCTTATGTATGTTGCTACAATGGATTGCGTCCCCAGGG 54.79%
Apta_1107 DNA GCGCGATGGACTGTACCTTCGGCGTAACTGATATTCCTGATGATTCTGGCGAGACGTAGCACAAACCCGGTGTATA 53.95%
Apta_357 DNA ACAATGTCGCTCTCCGCCGCAGGAGCATTGTCTGTCTTTATGCTTCTCTTTTTTGTTGC 53.42%
Apta_882 DNA AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG 53.42%
Apta_878 DNA TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGTGGGTGGGCTTCGCACACACGGACTTAC 53.16%
Apta_1109 DNA GGTATGTGTCGTTGATCTGGATATCTATGCCTAATGCTCGTAGGCGACTGTTCGGATAGTAACGTTGACCGCATAC 52.63%
Apta_495 DNA GGGAATGGATCCACATCTACGTATTACTGCTGGTGACGAGGGCTAGACGACGTACCTTCACTGCAGACTTGACGAAGCTT 52.50%
Apta_841 DNA TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC 52.50%
Apta_76 DNA CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG 52.05%
Apta_617 DNA CAATGGGGTCGGGCGGGCCGGGTGTCATGGTGGACGGAGATGGGACGTAGAGGGCGGT 52.05%
Apta_943 DNA GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT 52.05%
Apta_1035 DNA ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC 52.05%
Apta_1172 DNA GACGCCACTGACTACCCCAGTATCTCTTCAACGCGTATTGAAGTCAGTCGGTCGTCA 52.05%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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