AptaDB: Result

Interaction ID: 276

Aptamer sequence: CUGUCGAGCAUGCUCAGGGUAACGUAUAGUCAAGACACCUCAAGUGUAACU

Target unique ID: P09038

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_271	28B	Protein	FGF2_HUMAN	0.32 nM	1X PBS (10.1 mM Na2HPO4/1.8 mM KH2PO4/137 mM NaCl/2.7 mM KCl, pH 7.4)	7504300

Aptamer Sequence:	CUGUCGAGCAUGCUCAGGGUAACGUAUAGUCAAGACACCUCAAGUGUAACU
The optimal secondary structure in dot-bracket notation:	.((((((..((((..........))))..))..))))..............
The centroid secondary structure in dot-bracket notation:	...................................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_271	Description	28B
Aptamer chemistry	RNA	Length	51 nt
GC content	47.1%	Molecular weight	16,594.70 Da
Molarity of 1 μg/μl solution	60.26 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	CUGUCGAGCAUGCUCAGGGUAACGUAUAGUCAAGACACCUCAAGUGUAACU
Applications	Inhibit the first step in the signaling pathway of basic fibroblast growth factor: binding of the growth factor to Its cell-surface receptors

Type	Detail	Type	Detail
Uniprot ID	P09038	Protein name	Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Gene name(s)	FGF2 FGFB	Organism	Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Status	reviewed in uniprot database	Length	288
Mass	30,770	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	1BAS;1BFB;1BFC;1BFF;1BFG;1BLA;1BLD;1CVS;1EV2;1FGA;1FQ9;1II4;1IIL;2BFH;2FGF;2M49;4FGF;4OEE;4OEF;4OEG;5X1O;6L4O;
Function	Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4.

	Interaction ID	276
	Target type	Protein
	Target unique ID	P09038
	Activity	0.32 nM
	Binding Conditions/Buffer	1X PBS (10.1 mM Na2HPO4/1.8 mM KH2PO4/137 mM NaCl/2.7 mM KCl, pH 7.4)
	Assay	Representative binding curves for high-affinity RNA ligands
	PubMed ID	7504300

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_270	RNA	CUGUCGAACAUGCUGAGGGUAACGUACUGGCAAGCUCACCUCAGCGUAGCUAa	77.36%
Apta_269	RNA	AACGCUCAACGUCAGAAGGCAACGUAUAGGCAAGCACACUUCGACAUGAGGC	57.69%
Apta_85	RNA	UGCGACUGUUAUAACCUAACAGCGACGUAAAGAUA	54.90%
Apta_95	RNA	CCGCGACAGUCGUAAGUUUUGACUGACUGAACGUU	54.90%
Apta_97	RNA	UUGUCGUCUUCUCCAGUUAGUCAACGAACUUGUGU	54.90%
Apta_645	RNA	CUGGACGACGGGGACGCCACUGGACUAGGUAAGCCAGGACCGUACGUCGGGAGCCGUCAGAAUA	53.12%
Apta_93	RNA	CUCCGCUAUCUAACGUAUGAUAGAAUGGAUGACUA	52.94%
Apta_388	RNA	GCGGAAGCGUGCUGGGCUAGAAUAAUAAUAAGAAAACCAGUACUUUCGU	52.94%
Apta_268	RNA	CUUAUAGGGGAGGCUGUGGUGAGGGAAUAUUAAGAGAAUUAACGGUCUAGUUCACCUCGA	51.67%
Apta_136	RNA	CGUGUCGUCCUGGUACGAUUUGGUAUAUAACCGUGGCUUUUCGACA	50.98%
Apta_243	RNA	GCUUGAAGCUAGCAGUAAGACUAGCGCUACGGAUGGGUGUGCG	50.98%
Apta_658	RNA	CUGCGAUCAGGGGUAAAUUUCCGCGCAGGCUCCACGCCGC	50.98%
Apta_758	RNA	GGCUUUCGGGCUUUCGGCAACAUCAGCCCCUCAGCC	50.98%
Apta_859	RNA	GGGAGUCUGAAGUCGGACUUGUUAUCAAUUCACUUCAGACU	50.98%
Apta_1123	RNA	AUUCGGGCCCAGGAACCAACUAUAUAAAUGUCCCGAAUGCUUCGACG	50.98%
Apta_447	RNA	ACGCUCAACGAGCCAGGAACAUCGACGUCAGCAAACGCGAGCGCAACCAGUAACACC	50.88%
Apta_301	RNA	AUACCAGCUUAUUCAAUUGCCUGAAAAGUUGAACUCCAAAUACGCGCUGAGAUAGUAAGUGCAAUCU	50.75%
Apta_256	RNA	GGGCGCUAAGUCCUCGCUCAUGCGCGUCCCAUGGGGUAUAGAGGGGUCGAAGUGGACGCGCGACUCGGAUCCU	50.68%
Apta_294	RNA	AUACCAGCUUAUUCAAUUGCCUGAUUAGCGGUAUCACGAUUACUUACCUUCGUUGCUGAGAUAGUAAGUGCAAUCU	50.00%
Apta_299	RNA	AUACCAGCUUAUUCAAUUGCCUGAAGACUGGAUAUACUCUUAAGCAUUUCUAUAAUCGAGAUAGUAAGUGCAAUCU	50.00%

ABOUT