Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 357
Aptamer sequence: GGGAGGACGAUGCGGUGGUCUCCCAAUUCUAAACUUUCUCCAUCGUAUCUGGGCAGACGACUCGCCCGA
Target unique ID: P21781
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_352 14F Protein FGF7_HUMAN 0.3 pM DPBS, 0.5 mM MgCl2, 0.2% BSA, 0.02% sodium azide for PC-3, serum-free MEM growth medium, 0.1% BSA, 25 mM HEPES (pH 7.4), 1 ug/ml heparin for NIH3T3/KGFR 9035109
Structure information of aptamer
Aptamer Sequence: GGGAGGACGAUGCGGUGGUCUCCCAAUUCUAAACUUUCUCCAUCGUAUCUGGGCAGACGACUCGCCCGA
The optimal secondary structure in dot-bracket notation: ........((((((((((.....................))))))))))(((((((....)).))))).
The centroid secondary structure in dot-bracket notation: ........((((((((((.....................))))))))))(((((.........))))).
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_352 Description 14F
Aptamer chemistry RNA Length 69 nt
GC content 56.5% Molecular weight 21,468.51 Da
Molarity of 1 μg/μl solution 46.58 μM Number of G-quadruplexes 2
G-Score 17 Function Targeted binding
Sequence GGGAGGACGAUGCGGUGGUCUCCCAAUUCUAAACUUUCUCCAUCGUAUCUGGGCAGACGACUCGCCCGA
Applications Binding to keratinocyte growth factor
Target information
Type Detail Type Detail
Uniprot ID P21781 Protein name Fibroblast growth factor 7 (FGF-7) (Heparin-binding growth factor 7) (HBGF-7) (Keratinocyte growth factor)
Gene name(s) FGF7 KGF Organism Fibroblast growth factor 7 (FGF-7) (Heparin-binding growth factor 7) (HBGF-7) (Keratinocyte growth factor)
Status reviewed in uniprot database Length 194
Mass 22,509 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
N/A
Function Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation.
Activity data

No relevant experimental diagram

 Interaction ID 357
Target type Protein
Target unique ID P21781
Activity 0.3 pM
Binding Conditions/Buffer

DPBS, 0.5 mM MgCl2, 0.2% BSA, 0.02% sodium azide for PC-3, serum-free MEM growth medium, 0.1% BSA, 25 mM HEPES (pH 7.4), 1 ug/ml heparin for NIH3T3/KGFR
Assay

N/A
PubMed ID 9035109
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_984 RNA GGGAGGACGAUGCGGCGUAUUGCGCGAGGAUUAUCCGCUCAUCGUUGUUGUUGUGCAGACGACUCGCCCGA 69.01%
Apta_130 RNA GGGAGGACGAUGCGGUGGUAGCGCGAUAUAGCGCUGGUAGGGUUGCCGGUGAUCAGACGACUCGCCCGA 68.12%
Apta_133 RNA GGGAGGACGAUGCGGUGAGUCGUUCCCUUCGUCCCCAGACGACUCGCCCGA 66.67%
Apta_315 RNA GGGAGGACGAUGCGGUCGUAUUAUCCGCUGCACGCCAGACGACUCGCCCGA 66.67%
Apta_131 RNA GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 65.22%
Apta_132 RNA GGGAGGACGAUGCGGCGUUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA 65.22%
Apta_134 RNA GGGAGGACGAUGCGGGCCGUUGUUGUGUGCCGCCCCAGACGACUCGCCCGA 63.77%
Apta_467 RNA GGGAGGACGAUGCGGCAGUGCCCAACCGGAACAACAACCACCGGCGGCUCCUGCUCAGACGACUCGCUGAGGAUCCGAGA 62.50%
Apta_135 RNA GGGAGGACGAUGCGGAUUCUGGUUACUGGCCGCCCCAGACGACUCGCCCGA 62.32%
Apta_396 RNA GGGAGGACGAUGCGGCAGUCUGCAUCGUAGGAAUCGCCACCGUAUACUUUCCCACCAGACGACUCGCUGAGGAUCCGAGA 61.25%
Apta_414 RNA GGGAGGACGAUGCGGCCGAUUAGAACUGGGCUGAGGCGUUCUGCAUUUCGGUGAUCAGACGACUCGCUGAGGAUCCGAGA 60.00%
Apta_415 RNA GGGAGGACGAUGCGGAAACUCAUCGGUAGCCUUCCUGCGGUCAGUCUAUUAGGACCAGACGACUCGCUGAGGAUCCGAGA 60.00%
Apta_399 RNA GGGAGGACGAUGCGGCAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGGCAGACGACUCGCUGAGGAUCCGAGA 59.49%
Apta_398 RNA GGGAGGACGAUGCGGCUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCACAGACGACUCGCUGAGGAUCCGAGA 57.50%
Apta_305 RNA GGGAGGACGAUGCGGAUCGCCCUGAACCGGCCCAGCAGACUGCUGACGGCACGAUCCGCAUCGUCCUCCC 57.14%
Apta_129 RNA GGGAGGACGAUGCGGACACCGUUAAUCUGAGGCCCUGUCCUAUUCCUUCACGCCUCAGA 56.52%
Apta_397 RNA GGGAGGACGAUGCGGAUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCUCAGACGACUCGCUGAGGAUCCGAGA 56.25%
Apta_400 RNA GGGAGGACGAUGCGGAUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAACAGACGACUCGCUGAGGAUCCGAGA 55.70%
Apta_1056 RNA GUCAUGUUGUGUUGUGGUCCAUCCUUUUCUACGCCAGUCGAGUCUGUAGCGUGGGAGUAAACUGAACGGUCAGACAGGA 54.43%
Apta_43 RNA GGGAGAGCGGAAGCGUGCUGGGCCACCAUCCGUAACUAGCUAAUACUUGUUAUCUUUUUAUUUUCAUAACCCAGAGGUCGAUGGAUCCCCCC 53.26%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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