AptaDB: Result

Interaction ID: 467

Aptamer sequence: GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA

Target unique ID: Q4PRK9

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_462	1501s	Protein	Q4PRK9_PLAVI	670 nM	100 mM Tris-HCl, 10 mM imidazole, and 0.3 M NaCl, pH 8	32631977

Aptamer Sequence:	GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA
The optimal secondary structure in dot-bracket notation:	(((....))).(((.(((((....))))).)))..
The centroid secondary structure in dot-bracket notation:	(((....))).(((.(((((....))))).)))..

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_462	Description	1501s
Aptamer chemistry	DNA	Length	35 nt
GC content	62.9%	Molecular weight	10,766.92 Da
Molarity of 1 μg/μl solution	92.88 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA
Applications	Recognition of a malaria biomarker

Type	Detail	Type	Detail
Uniprot ID	Q4PRK9	Protein name	L-lactate dehydrogenase
Gene name(s)	LDH ldh PVC01_120033300 PVP01_1229700 PVT01_120033900	Organism	L-lactate dehydrogenase
Status	unreviewed in uniprot database	Length	316
Mass	34,223	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=(S)-lactate + NAD(+) = H(+) + NADH + pyruvate
PDB ID(s)	2A92;2AA3;5HRU;5HS4;5HTO;6TXR;
Function	N/A

	Interaction ID	467
	Target type	Protein
	Target unique ID	Q4PRK9
	Activity	670 nM
	Binding Conditions/Buffer	100 mM Tris-HCl, 10 mM imidazole, and 0.3 M NaCl, pH 8
	Assay	SPR (Biacore) analysis of the interaction between 1501s and PvLDH. PvLDH was immobilized to a Ni-NTA chip, then the response was observed at multiple concentrations of 1501s as shown in the figure. Raw data are shown background with the best fit overlain with a thick solid line. For 1501s binding to PvLDH, the association rate (kon), dissociation rate (koff), and the equilibrium dissociation constant (KD) were 2.2 × 104 M-1·s-1 , 1.4 × 10-2 s-1 , and 670 nM, respectively
	PubMed ID	32631977

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_60	DNA	GGAATGAGTGCCCTGCAAGCGAGGGCTAGC	60.00%
Apta_182	DNA	AAAAAAGCCCCCCCTTCGACCGTATACCAGGGTACGGAATG	56.10%
Apta_163	DNA	GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG	55.26%
Apta_189	DNA	AGGAACCCTTTACCTCTGAGATCTCGGAAT	54.29%
Apta_1094	DNA	GGAATCGTGGGTGGTAGGGTAGGGGATGCA	54.29%
Apta_164	DNA	TATAGACCACAGCCTGAGATTAACCACCAACCCAGGACT	53.85%
Apta_818	DNA	GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA	53.33%
Apta_165	DNA	TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA	52.50%
Apta_259	DNA	GCAAGTATGAGCGCAGGAGTTAGGTCCCGTGGCGATGGGT	52.50%
Apta_273	DNA	ATAAGCTGAGCATCTTAGATCCCCGTCAAGGGCAGCGTAA	52.50%
Apta_385	DNA	ACAGATACGGAACTACAGAGGTCAATTACGGTGGCCACGC	52.50%
Apta_386	DNA	GGAAAAGATGGTATGGGCCCTAGAAAGTCCACCCGGCGCC	52.50%
Apta_817	DNA	TGCGTGAGCGGTAGGCCCCTACGACCCACTGTGGTTGGGC	52.50%
Apta_965	DNA	CACGGCATGTGTGGCCGGATGCCTATATCGGTCGACTGCA	52.50%
Apta_967	DNA	GGGTAGCAGCCCCGCGGAGGGTCGGCTATAAGAACCAAGA	52.50%
Apta_556	DNA	GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC	52.08%
Apta_190	DNA	AGGAACCCTTTAGGTCTGAGATCTCGGAAT	51.43%
Apta_191	DNA	AGGAAGGCTTTACCTCTGAGATCTCGGAAT	51.43%
Apta_192	DNA	AGGAAGGCTTTAGCTCTGAGATCTCGGAAT	51.43%
Apta_203	DNA	GGAAGGCTTTAGGTCTGAGATCTCGG	51.43%

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