Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 131
Aptamer sequence: GGGAGGACGAUGCGGUGGUAGCGCGAUAUAGCGCUGGUAGGGUUGCCGGUGAUCAGACGACUCGCCCGA
Target unique ID: P01579
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_130 | 2'NH2-17 | Protein | IFNG_HUMAN | 1.7 nM | PBS (138 mM NaC1, 2.7 mM KCl, 8.1 mM Na2HPO4, and 1.1 mM KH2PO4, pH 7.4) containing 1 mM Mg2+ ions and 0.01% human serum albumin | 9200462 |
Structure information of aptamer
| Aptamer Sequence: | GGGAGGACGAUGCGGUGGUAGCGCGAUAUAGCGCUGGUAGGGUUGCCGGUGAUCAGACGACUCGCCCGA |
| The optimal secondary structure in dot-bracket notation: | ....((.((((....((.((((((......)))))).))..))))))(((((((....)).)))))... |
| The centroid secondary structure in dot-bracket notation: | ....((.((((....((.((((((......)))))).))..))))))(((((((....)).)))))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_130 | Description | 2'NH2-17 |
| Aptamer chemistry | RNA | Length | 69 nt |
| GC content | 63.8% | Molecular weight | 22,720.33 Da |
| Molarity of 1 μg/μl solution | 44.01 μM | Number of G-quadruplexes | 2 |
| G-Score | 17 | Function | Targeted binding |
| Sequence | GGGAGGACGAUGCGGUGGUAGCGCGAUAUAGCGCUGGUAGGGUUGCCGGUGAUCAGACGACUCGCCCGA | ||
| Applications | Binding to target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P01579 | Protein name | Interferon gamma (IFN-gamma) (Immune interferon) |
| Gene name(s) | IFNG | Organism | Interferon gamma (IFN-gamma) (Immune interferon) |
| Status | reviewed in uniprot database | Length | 166 |
| Mass | 19,348 | Activity regulation | N/A |
| Involvement in disease | Aplastic anemia (AA) | Catalytic activity | N/A |
| PDB ID(s) | 1EKU;1FG9;1FYH;1HIG;3BES;6E3K;6E3L; |
 |
Function | Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 131 |
| Target type | Protein | |
| Target unique ID | P01579 | |
| Activity | 1.7 nM | |
| Binding Conditions/Buffer | PBS (138 mM NaC1, 2.7 mM KCl, 8.1 mM Na2HPO4, and 1.1 mM KH2PO4, pH 7.4) containing 1 mM Mg2+ ions and 0.01% human serum albumin |
|
| Assay | N/A |
|
| PubMed ID | 9200462 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_984 | RNA | GGGAGGACGAUGCGGCGUAUUGCGCGAGGAUUAUCCGCUCAUCGUUGUUGUUGUGCAGACGACUCGCCCGA | |
| Apta_133 | RNA | GGGAGGACGAUGCGGUGAGUCGUUCCCUUCGUCCCCAGACGACUCGCCCGA | |
| Apta_134 | RNA | GGGAGGACGAUGCGGGCCGUUGUUGUGUGCCGCCCCAGACGACUCGCCCGA | |
| Apta_352 | RNA | GGGAGGACGAUGCGGUGGUCUCCCAAUUCUAAACUUUCUCCAUCGUAUCUGGGCAGACGACUCGCCCGA | |
| Apta_131 | RNA | GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA | |
| Apta_132 | RNA | GGGAGGACGAUGCGGCGUUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA | |
| Apta_315 | RNA | GGGAGGACGAUGCGGUCGUAUUAUCCGCUGCACGCCAGACGACUCGCCCGA | |
| Apta_135 | RNA | GGGAGGACGAUGCGGAUUCUGGUUACUGGCCGCCCCAGACGACUCGCCCGA | |
| Apta_414 | RNA | GGGAGGACGAUGCGGCCGAUUAGAACUGGGCUGAGGCGUUCUGCAUUUCGGUGAUCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_400 | RNA | GGGAGGACGAUGCGGAUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAACAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_397 | RNA | GGGAGGACGAUGCGGAUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCUCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_398 | RNA | GGGAGGACGAUGCGGCUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCACAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_396 | RNA | GGGAGGACGAUGCGGCAGUCUGCAUCGUAGGAAUCGCCACCGUAUACUUUCCCACCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_467 | RNA | GGGAGGACGAUGCGGCAGUGCCCAACCGGAACAACAACCACCGGCGGCUCCUGCUCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_399 | RNA | GGGAGGACGAUGCGGCAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGGCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_129 | RNA | GGGAGGACGAUGCGGACACCGUUAAUCUGAGGCCCUGUCCUAUUCCUUCACGCCUCAGA | |
| Apta_415 | RNA | GGGAGGACGAUGCGGAAACUCAUCGGUAGCCUUCCUGCGGUCAGUCUAUUAGGACCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_305 | RNA | GGGAGGACGAUGCGGAUCGCCCUGAACCGGCCCAGCAGACUGCUGACGGCACGAUCCGCAUCGUCCUCCC | |
| Apta_594 | RNA | GGGCUAUUGCUGGAGGGGCGCCACAUGAAAGUGGUGGUUGGGUGCGGUCGGCGAUAGCUC | |
| Apta_718 | RNA | GGGUUGUGAGCUCCCGGCUCGCAUUGUGAGGUGGAGGGGGACGUGCUGCAGGUCGACGCAUGCGCCG |
DEV TOOL
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