AptaDB: Result

Interaction ID: 299

Aptamer sequence: GAACTCACCGGGAAGAAGCCCGTTCCGTCACAGACATGTT

Target unique ID: P10148

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_290	ADR7	Protein	CCL2_MOUSE	180 pM	110 mM NaCl, 1 mM MgCl2, 20 mM HEPES (pH 7.0), 1 mM CaCl2, 5 mM KCl, 0.001% HSA, 0.05% Tween 20	11591377

Aptamer Sequence:	GAACTCACCGGGAAGAAGCCCGTTCCGTCACAGACATGTT
The optimal secondary structure in dot-bracket notation:	........((((......))))....(((...))).....
The centroid secondary structure in dot-bracket notation:	........((((......))))..................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_290	Description	ADR7
Aptamer chemistry	DNA	Length	41 nt
GC content	55.0%	Molecular weight	12,514.84 Da
Molarity of 1 μg/μl solution	79.91 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	GAACTCACCGGGAAGAAGCCCGTTCCGTCACAGACATGTT
Applications	Recognise MCP-1 in different ways

Type	Detail	Type	Detail
Uniprot ID	P10148	Protein name	C-C motif chemokine 2 (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Platelet-derived growth factor-inducible protein JE) (Small-inducible cytokine A2)
Gene name(s)	Ccl2 Je Mcp1 Scya2	Organism	C-C motif chemokine 2 (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Platelet-derived growth factor-inducible protein JE) (Small-inducible cytokine A2)
Status	reviewed in uniprot database	Length	148
Mass	16,326	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Acts as a ligand for C-C chemokine receptor CCR2 (By similarity).

	Interaction ID	299
	Target type	Protein
	Target unique ID	P10148
	Activity	180 pM
	Binding Conditions/Buffer	110 mM NaCl, 1 mM MgCl2, 20 mM HEPES (pH 7.0), 1 mM CaCl2, 5 mM KCl, 0.001% HSA, 0.05% Tween 20
	Assay	Aptamer inhibition of heparin binding. Binding measurements were carried out by surface plasmon resonance using a Biacore 2000 instrument at 25 ℃
	PubMed ID	11591377

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_498	DNA	GAACTCATTGGCTGGAGGCGGCAGTACCGCTTGAGTT	57.50%
Apta_593	DNA	CTCTCGGGACGACAGCCGAGTTGATTCAACAGCCGAGTCGTCCC	54.55%
Apta_434	DNA	ACACGACGGGAATACTGACTCTCCCCCATGT	52.50%
Apta_722	DNA	CTCTCGGGACGACGGCCCGATCTCAGAGTAGTCGTCCC	52.50%
Apta_1063	DNA	AACTTATCGGAGCGTGATTTCCGTCTCGCCGCTTTCCTTT	52.50%
Apta_1065	DNA	CCTCGCTATGATGGAGTGCGTTTAGATCAGGGAACGGGTT	52.50%
Apta_556	DNA	GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC	52.08%
Apta_891	DNA	GGAGACCGTACCATCTGTTCGTGGAAGCGCTTTGCTCGTCCATTAGCCTTGTGCTCGTGC	51.67%
Apta_666	DNA	TCGACCTCGCGCGAGGAGGGTGGAGGGTCGTAGAGCGCGTA	51.22%
Apta_968	DNA	TAACACGACAGACGTTCGGAGGTCGAACCCTGACAGCGTGGGC	51.16%
Apta_987	DNA	GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC	51.06%
Apta_1162	DNA	AGAGGCTACAGCGATAAGTCGACATTGCTGACCGTACCTAGTAATACGT	51.02%
Apta_1127	DNA	TCGGGCGAGTCGTCTGCGAGGCAACAAGCTCGTAATCCGCATCGTCCTCCC	50.98%
Apta_139	DNA	GATAAACGCCTTGATTAAAGGCCCAGTTCTTTAGGCCTACACGTGCTGCGACATTAATT	50.85%
Apta_155	RNA	GACCCAGGGCCGGGACGCAAAGCAGCCAAAACAGACGGCCCCAGUCAGGGAGUU	50.00%
Apta_163	DNA	GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG	50.00%
Apta_274	DNA	GATACTGAGCATCGTACATGATCCCGCAACGGGCAGTATT	50.00%
Apta_291	DNA	AAAAATCTAGGCGCTGAAGCCCCGCTTCCTTACCTTCAC	50.00%
Apta_302	DNA	ATCCTGTGAGGAATGCTCATGCATAGCAAGGGCT	50.00%
Apta_406	DNA	AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT	50.00%

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