Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1063
Aptamer sequence: AACGAGCACGCTTTGTGTACAGCCACTAAATAAGGTATGTAGTCAGCACCCTCGTAAC
Target unique ID: P04640
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_980 OC12 Protein OSTCN_RAT 95.05 nM 2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water 33379043
Structure information of aptamer
Aptamer Sequence: AACGAGCACGCTTTGTGTACAGCCACTAAATAAGGTATGTAGTCAGCACCCTCGTAAC
The optimal secondary structure in dot-bracket notation: .(((((...(((.....(((((((.........))).))))...)))...)))))...
The centroid secondary structure in dot-bracket notation: .(((((...(((..(..(((((((.........))).))))..))))...)))))...
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_980 Description OC12
Aptamer chemistry DNA Length 58 nt
GC content 46.6% Molecular weight 17,818.47 Da
Molarity of 1 μg/μl solution 56.12 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Aptasensor
Sequence AACGAGCACGCTTTGTGTACAGCCACTAAATAAGGTATGTAGTCAGCACCCTCGTAAC
Applications Binding to target proteins
Target information
Type Detail Type Detail
Uniprot ID P04640 Protein name Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
Gene name(s) Bglap Bglap2 Organism Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
Status reviewed in uniprot database Length 99
Mass 10,927 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
N/A
Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Activity data
Interaction ID 1063
Target type Protein
Target unique ID P04640
Activity 95.05 nM
Binding Conditions/Buffer

2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water
Assay

Determination of affinity/dissociation constant (Kd) of from the saturation binding curve
PubMed ID 33379043
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_931 DNA ATACGACTCACTATTAGGGACCACATAAACATGCATCGCTCAATAACGGGATATTGTTGTGCTAGACTACTGACTACAA 55.70%
Apta_108 DNA AGCAGCACAGAGGTCAGATGCTTTGCCACGGCAACGTCCCAAAACTAATGGTAACTATACCCTATGCGTGCTACCGTGAA 53.75%
Apta_108 DNA AGCAGCACAGAGGTCAGATGCTTTGCCACGGCAACGTCCCAAAACTAATGGTAACTATACCCTATGCGTGCTACCGTGAA 53.75%
Apta_440 DNA AAAGACCGTTTTTTAAAACGCTCAATATACACGACATAAA 53.45%
Apta_445 DNA AGCCATGACGATGTCGTTACGTAGATGTAGAGACTCCTAA 53.45%
Apta_585 DNA GATCGAGGGCAGCGATAGCTGGGCTAATAAGGTTAGCCCCATCGGTC 53.45%
Apta_779 DNA TACGGGCTGGATCCACTGTTACGGCGTGTATCCGCTATCAA 53.45%
Apta_1127 DNA TCGGGCGAGTCGTCTGCGAGGCAACAAGCTCGTAATCCGCATCGTCCTCCC 53.45%
Apta_932 DNA ATACGACTCACTATTAGGGATGTCCACCATATAGATCGATTTAAGTCCCTCGTTATTAATGCTAGACTACTGACTACAA 53.16%
Apta_999 DNA ATAGGAGTCGACCGACCAGAGGACCCCGCTATCTAATTTATAGGCAATAAGTGCGTCTACATCTAGACTC 52.86%
Apta_175 DNA AGCAGCACAGAGGTCAGATGAGGACTGTCGATAAGTATAATGGCAAGTAGCTCGATACATCCTATGCGTGCTACCGTGAA 52.50%
Apta_803 DNA ATGAGAAGCGTCGGTGTGGTTACTCCGGGCACTTGATATATCGATATGGAGAATAATGCACCCTGAGCGGGCTGGCAAGGCGCATA 52.33%
Apta_67 DNA AGATGCCTGTCGAGCATGCTGTTGTGGTAGGGTTAGGGATGGTAGCGGTTGTAGCTAAACTGCTTTGTCGACGGG 52.00%
Apta_944 DNA ATACGACTCACTATTAGGGACAAAGCTGACAACCCTTTCATAATCTAACTACATTTATGTGCTAGACTACTGACTAC 51.95%
Apta_395 DNA AATCCACGTATCGACTAGCTCAAACCAGCCTGATAGAGCCATTCGCTTTA 51.72%
Apta_421 DNA ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA 51.72%
Apta_438 DNA AGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA 51.72%
Apta_438 DNA AGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA 51.72%
Apta_444 DNA AGCCATTACGATGTCGTTACGTAGATGCAGAGACTCCTAA 51.72%
Apta_446 DNA AGCCATGACGATGTCGTTACGTAGATGCATAGACTCCTAA 51.72%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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