AptaDB: Result

Interaction ID: 163

Aptamer sequence: GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT

Target unique ID: P04183

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_159	TK1_apta69	Protein	KITH_HUMAN	510 pM	PBS	30900869

Aptamer Sequence:	GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT
The optimal secondary structure in dot-bracket notation:	..(((..((((........))))..)))...........
The centroid secondary structure in dot-bracket notation:	..(((..((((........))))..)))...........

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_159	Description	TK1_apta69
Aptamer chemistry	DNA	Length	39 nt
GC content	48.7%	Molecular weight	12,224.67 Da
Molarity of 1 μg/μl solution	81.80 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT
Applications	Diagnostic assays

Type	Detail	Type	Detail
Uniprot ID	P04183	Protein name	Thymidine kinase, cytosolic (EC 2.7.1.21)
Gene name(s)	TK1	Organism	Thymidine kinase, cytosolic (EC 2.7.1.21)
Status	reviewed in uniprot database	Length	234
Mass	25,469	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=ATP + thymidine = ADP + dTMP + H(+)
PDB ID(s)	1W4R;1XBT;2ORV;2WVJ;
Function	Cell-cycle-regulated enzyme of importance in nucleotide metabolism.

No relevant experimental diagram	Interaction ID	163
	Target type	Protein
	Target unique ID	P04183
	Activity	510 pM
	Binding Conditions/Buffer	PBS
	Assay	N/A
	PubMed ID	30900869

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_818	DNA	GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA	62.22%
Apta_161	DNA	GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC	58.97%
Apta_163	DNA	GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG	56.41%
Apta_260	DNA	GACGTTAAGCTAGCAGGTGTTAGGTCCCGTGGTGATGAAT	55.00%
Apta_943	DNA	GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT	54.00%
Apta_975	DNA	AGACATAGTACATAACCGATCAATGAACATTTAATTAAATGGTGGTATCC	54.00%
Apta_678	DNA	CGTACGGTGGGAACGGTTCCTCTTAGGGT	53.85%
Apta_1033	DNA	CACAGGTTCCAGGTAATACCTAAGGGTATGCTCTCGCCTATTATATGGAGCAC	52.83%
Apta_221	DNA	GTCCTAGCAGACACTCGGCCATCACGCCCATTGGTTTGCA	52.50%
Apta_473	DNA	CCCTGCACTCGCAATGGCCCCTCTACACCACAAACGGACA	52.50%
Apta_817	DNA	TGCGTGAGCGGTAGGCCCCTACGACCCACTGTGGTTGGGC	52.50%
Apta_1287	DNA	GCCCGCATGCCGTCAAGCAATGTTAATCTTGTTTTGATTTCG	52.38%
Apta_974	DNA	CATGGTGTCAATACTATACCTTTTGCCGATGGTAACAGTTGGTGGTATCC	52.00%
Apta_1045	DNA	GGTGCTCAGGGAACTGTCTGAGGGATCAGGCTTAAGCCTGTCGAGCAGTT	52.00%
Apta_162	DNA	CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG	51.28%
Apta_191	DNA	AGGAAGGCTTTACCTCTGAGATCTCGGAAT	51.28%
Apta_325	DNA	TACTGCATGCACACCACTTCAACTA	51.28%
Apta_325	DNA	TACTGCATGCACACCACTTCAACTA	51.28%
Apta_588	RNA	CCGGUGCGCAUAACCACCUCAGUGCGAGCAAGGAA	51.28%
Apta_917	DNA	TCGATTTCCTTAGTTGTCTTCCTTAGTGAG	51.28%

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