Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 251
Aptamer sequence: GGCTTTACACTTTATGCTTCCGGCCCGCGGAAATGATAACGCGTATGGTGGTATAACAATTTCACACAGGAAACAG
Target unique ID: P08246
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_246 | BnHNE1 | Protein | ELNE_HUMAN | 32.5 nM | 20 mM HEPES pH 7.5, 150 mM NaCl, 6 mM KCl, 2 mM MgCl2, room temperature | 31961667 |
Structure information of aptamer
| Aptamer Sequence: | GGCTTTACACTTTATGCTTCCGGCCCGCGGAAATGATAACGCGTATGGTGGTATAACAATTTCACACAGGAAACAG |
| The optimal secondary structure in dot-bracket notation: | ...........(((((((.(((...((((..........))))..))).)))))))...((((......))))... |
| The centroid secondary structure in dot-bracket notation: | ...........(((((((.(((...((((..........))))..))).)))))))....(((......))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_246 | Description | BnHNE1 |
| Aptamer chemistry | DNA | Length | 76 nt |
| GC content | 46.1% | Molecular weight | 23,689.99 Da |
| Molarity of 1 μg/μl solution | 42.21 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | GGCTTTACACTTTATGCTTCCGGCCCGCGGAAATGATAACGCGTATGGTGGTATAACAATTTCACACAGGAAACAG | ||
| Applications | Binding to target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P08246 | Protein name | Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) |
| Gene name(s) | ELANE ELA2 | Organism | Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) |
| Status | reviewed in uniprot database | Length | 267 |
| Mass | 28,518 | Activity regulation | N/A |
| Involvement in disease | Cyclic haematopoiesis (CH) | Catalytic activity | Reaction=Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa |
| PDB ID(s) | 1B0F;1H1B;1HNE;1PPF;1PPG;2RG3;2Z7F;3Q76;3Q77;4NZL;4WVP;5A09;5A0A;5A0B;5A0C;5A8X;5A8Y;5A8Z;5ABW;6E69;6F5M;6SMA;7CBK; |
 |
Function | Modifies the functions of natural killer cells, monocytes and granulocytes. |
Activity data
 |
Interaction ID | 251 |
| Target type | Protein | |
| Target unique ID | P08246 | |
| Activity | 32.5 nM | |
| Binding Conditions/Buffer | 20 mM HEPES pH 7.5, 150 mM NaCl, 6 mM KCl, 2 mM MgCl2, room temperature |
|
| Assay | Binding of BnHNE1, BnHNE2, BnHNE3, and their natural counterparts to HNE. Data are average ± SD of three independent determinations |
|
| PubMed ID | 31961667 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_248 | DNA | GGCTTTACACTTTATGCTTCCGGCCGAGGATTTTCATACCCCGCACGAACGCATAACAATTTCACACAGGAAACAG | |
| Apta_247 | DNA | GGCTTTACACTTTATGCTTCCGTACCAGAACATATCGAGTGATCCTGGGAGGATAACAATTTCACACAGGAAACAG | |
| Apta_84 | DNA | GGCTTTACACTTTATGCTTCCGTTGCAATTTTAATGGGAGGTACATGGCGGAATAACAATTTCACACAGGAAACAG | |
| Apta_82 | DNA | GGCTTTACACTTTATGCTTCCGAAACAACGCAGAAAGTCTTTACAACATTACATAACAATTTCACACAGGAAACAG | |
| Apta_83 | DNA | GGCTTTACACTTTATGCTTCCGTAAAAACACAAACACACGCACATACATAAAATAACAATTTCACACAGGAAACAG | |
| Apta_1135 | DNA | GCCGAGATTGCACTTACTATCTCATCAATAAAGATTATAGGGTGAAGGTGATAGTAGGTGGTATATAGGGTAATTGAATAAGCTGGTATGCGC | |
| Apta_980 | DNA | AACGAGCACGCTTTGTGTACAGCCACTAAATAAGGTATGTAGTCAGCACCCTCGTAAC | |
| Apta_226 | DNA | GGGAGATCACTTACGGCACCGTATAGTCGTACATGAACGAGTGTGAGTGTTAAGCCTTTGGCGACAGGGCTCGGAACC | |
| Apta_1015 | DNA | ATACCAGCTTATTCAATTGACCCGTTTCGTTCCCTCTGGGAAGTTTAGCCCAGTTGCCTGGGCGATACCAAGATAGTAAGTGCAATCT | |
| Apta_1132 | DNA | GCCGAGATTGCACTTACTATCTGAAAGGGTGGAGATTGATAGGTATAGAGAAACGATAAGGGGGTGTAGAGTAATTGAATAAGCTGGTATGCGC | |
| Apta_759 | DNA | GAGGCATACCAGCTTATTATTGGGGCCGGGGCAAGGGGGGGGTACCGTGGTAGGACAGATAGTAAGTGCAATCTGCGAA | |
| Apta_466 | DNA | AGCTGACACAGCAGGTTGGTGGGGGTGGCTCACATCATGGTAGAATGACTGAACAGCGTTGATTAAAAGGCACGAGTCGAGCAATCTCGAAAT | |
| Apta_454 | DNA | AGCTCCAGAAGATAAATTACAGGTGAGGGCGGGCGGGTGGTTGTAATATGATCGAATGGTATATGTGTGTTTGCAACTAGGATACTATGACCCCG | |
| Apta_823 | DNA | GGGATACCAGCTTATTCAATTTGATTCCATCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGGTTACGTATTTGGTTTATAGATAGTAAGTGCAATCT | |
| Apta_403 | DNA | GTCTTGACTAGTTACGCCCCCAAGAACCCGCTTGCCGTGGTGACGTTGATCATGTCGTTTGGTCATTCAGTTGGCGCCTC | |
| Apta_409 | DNA | GCAATGGTACGGTACTTCCGGATGCGGAAACTGGCTAATTGGTGAGGCTGGGGCGGTCGTGCAGCAAAAGTGCACGCTACTTTGCTAA | |
| Apta_455 | DNA | AGCTCCAGAAGATAAATTACAGGGGCCGGGGTGGCTCAGGCAAGGGGTTGACCTGTCGTAGGGATTGTTTTAACAACTAGGATACTATGACCCC | |
| Apta_1028 | DNA | CTTCTGCCCGCCTCCTTCCGGCGTCCCTGCGCACTGTATAACGTACACACGATACGTGGGAGACGAGATAGGCGGACACT | |
| Apta_1108 | DNA | GTGCTTAGGTGCAGAACGCACACACGCTCGGTATGACGTGTTCGCTACGGTATAATTGCAGGCGCGAGCACAACAG | |
| Apta_1130 | DNA | GCCGAGATTGCACTTACTATCTATTAGGAATAAGGTATGAATAGAGATGTATAAGTTGTGTGGACGGTGTAATTGAATAAGCTGGTATGCGC |
DEV TOOL
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