Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1130
Aptamer sequence: CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACACCCCCATAGGAGACGAGATAGGCGGACACT
Target unique ID: P01112
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1076 | aptamer4 for h-Ras | Protein | RASH_HUMAN | 420 nM | 50 mM Tris-HCl | 16448086 |
Structure information of aptamer
| Aptamer Sequence: | CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACACCCCCATAGGAGACGAGATAGGCGGACACT |
| The optimal secondary structure in dot-bracket notation: | ..((((((...(((.(((((((..(((((...................)))))..)))))))..)))...)))))).... |
| The centroid secondary structure in dot-bracket notation: | ..((((((...(((.(((((((..(((((...................)))))..)))))))..)))...)))))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1076 | Description | aptamer4 for h-Ras |
| Aptamer chemistry | DNA | Length | 80 nt |
| GC content | 53.8% | Molecular weight | 24,492.66 Da |
| Molarity of 1 μg/μl solution | 40.83 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACACCCCCATAGGAGACGAGATAGGCGGACACT | ||
| Applications | Applications in cancer research, development of cancer diagnostics, and treatment of cancer | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P01112 | Protein name | GTPase HRas (EC 3.6.5.2) (H-Ras-1) (Ha-Ras) (Transforming protein p21) (c-H-ras) (p21ras) [Cleaved into: GTPase HRas, N-terminally processed] |
| Gene name(s) | HRAS HRAS1 | Organism | GTPase HRas (EC 3.6.5.2) (H-Ras-1) (Ha-Ras) (Transforming protein p21) (c-H-ras) (p21ras) [Cleaved into: GTPase HRas, N-terminally processed] |
| Status | reviewed in uniprot database | Length | 189 |
| Mass | 21,298 | Activity regulation | Alternates between an inactive form bound to GDP and an active form bound to GTP |
| Involvement in disease | Costello syndrome (CSTLO) | Catalytic activity | Reaction=GTP + H2O = GDP + H(+) + phosphate |
| PDB ID(s) | 121P;1AA9;1AGP;1BKD;1CLU;1CRP;1CRQ;1CRR;1CTQ;1GNP;1GNQ;1GNR;1HE8;1IAQ;1IOZ;1JAH;1JAI;1K8R;1LF0;1LF5;1LFD;1NVU;1NVV;1NVW;1NVX;1P2S;1P2T;1P2U;1P2V;1PLJ;1PLK;1PLL;1Q21;1QRA;1RVD;1WQ1;1XCM;1XD2;1XJ0;1ZVQ;1ZW6;221P;2C5L;2CE2;2CL0;2CL6;2CL7;2CLC;2CLD;2EVW;2LCF;2LWI;2N42;2N46;2Q21;2QUZ;2RGA;2RGB;2RGC;2RGD;2RGE;2RGG;2UZI;2VH5;2X1V;3DDC;3I3S;3K8Y;3K9L;3K9N;3KKM;3KKN;3KUD;3L8Y;3L8Z;3LBH;3LBI;3LBN;3LO5;3OIU;3OIV;3OIW;3RRY;3RRZ;3RS0;3RS2;3RS3;3RS4;3RS5;3RS7;3RSL;3RSO;3TGP;421P;4DLR;4DLS;4DLT;4DLU;4DLV;4DLW;4DLX;4DLY;4DLZ;4DST;4DSU;4EFL;4EFM;4EFN;4G0N;4G3X;4K81;4L9S;4L9W;4NYI;4NYJ;4NYM;4Q21;4RSG;4URU;4URV;4URW;4URX;4URY;4URZ;4US0;4US1;4US2;4XVQ;4XVR;521P;5B2Z;5B30;5E95;5P21;5VBE;5VBZ;5WDO;5WDP;5WDQ;5WFO;5WFP;5WFQ;5WFR;5WPL;5X9S;5ZC6;621P;6AMB;6AXG;6BVI;6BVJ;6BVK;6BVL;6BVM;6CUO;6CUP;6CUR;6D55;6D56;6D59;6D5E;6D5G;6D5H;6D5J;6D5L;6D5M;6D5V;6D5W;6DZH;6E6C;6E6P;6MQT;6NTC;6NTD;6Q21;6V94;6V9F;6V9J;6V9L;6V9M;6V9N;6V9O;6ZJ0;6ZL3;721P;7DPH;7DPJ;7JHP;7JIF;7JIG;7JIH;7JII;7L0F;7L0G;7OG9;7OGA;7OGB;7OGC;7OGD;7OGE;7OGF;821P; |
 |
Function | Involved in the activation of Ras protein signal transduction. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 1130 |
| Target type | Protein | |
| Target unique ID | P01112 | |
| Activity | 420 nM | |
| Binding Conditions/Buffer | 50 mM Tris-HCl |
|
| Assay | N/A |
|
| PubMed ID | 16448086 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1073 | DNA | CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1075 | DNA | CTTCTGCCCGCCTCCTTCCTACTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1028 | DNA | CTTCTGCCCGCCTCCTTCCGGCGTCCCTGCGCACTGTATAACGTACACACGATACGTGGGAGACGAGATAGGCGGACACT | |
| Apta_1030 | DNA | CTTCTGCCCGCCTCCTTCCCGGGTTACAGGATCGTAAATCTTATTCGTTGCTCGATCGGGAGACGAGATAGGCGGACACT | |
| Apta_1074 | DNA | CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT | |
| Apta_1029 | DNA | CTTCTGCCCGCCTCCTTCCCGGTCGAGACCCGGGTTTACTTCCTCGGTAGTGTTCGAGGGAGACGAGATAGGCGGACACT | |
| Apta_737 | DNA | ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC | |
| Apta_1111 | DNA | GTGCGTCGCATGATCGCGATTAGTGCGACCATGTACGATTCTCACGACAACGACACTGTACTTGAGGACTGTCTCC | |
| Apta_448 | DNA | TAACTCACTCCATACTCACTTGCTGATTCGCCAACAACACACCCTTAAACAGTCCC | |
| Apta_534 | DNA | ATCCGTCACACCTGCTCTTGTAGGGTTCCCACCCAATTCAGTTCCGTTAAACCATGGTGTTGGCTCCCGTAT | |
| Apta_884 | DNA | ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT | |
| Apta_897 | DNA | ATACGGGAGCCAACACCACTCTTATTTCCGGAAGTGGTTCTTCAACAGAGGAATAGAGCAGGTGTGACGGAT | |
| Apta_534 | DNA | ATCCGTCACACCTGCTCTTGTAGGGTTCCCACCCAATTCAGTTCCGTTAAACCATGGTGTTGGCTCCCGTAT | |
| Apta_741 | DNA | ATCGTCTGCTCCGTCCAATAGCCTCACCCTGCATAATTTATAGACTACACTTAGGAATCGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_79 | DNA | ATCTCGAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACTCGT | |
| Apta_349 | DNA | ATACCAGCTTATTCAATTAGACGAGTTTTATTCATTTTCCATGTCGTAGCTTACCGTGAGATAGTAAGTGCAATCT | |
| Apta_354 | DNA | ATACCAGCTTATTCAATTCTATACTCCACTTTGCTATTTCTCGGTTCCTTCACGCGCCGATCGCAGGCTGATGAATTGAGATAGTAAGTGCAATCT | |
| Apta_435 | DNA | CCTTCGTTGTCTGCCTTCGTAGGACCGCAGACATCGACGCAGGGAAATTCCGCAAGTCCAGCCAAATGCCACCCTTCAGAATTCGCACCA | |
| Apta_526 | DNA | ATACGGGAGCCAACACCAAGTAACACACGCGGACCAGAAATACATCCCCCCGTAGAGCAGGTGTGACGGAT | |
| Apta_536 | DNA | ATCCGTCACACCTGCTCTCGAATAGAGTACTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT |
DEV TOOL
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