AptaDB: Result

Interaction ID: 454

Aptamer sequence: CGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCG

Target unique ID: Q15116

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_449	PD4S	Protein	PDCD1_HUMAN	10.3 nM	4.5 g/L glucose and 5 mM MgCl2 in Dulbecco's PBS without calcium and magnesium	35019367

Aptamer Sequence:	CGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCG
The optimal secondary structure in dot-bracket notation:	((((((((..(((....((((......)))))))..)).))))))
The centroid secondary structure in dot-bracket notation:	((((((((.........((((......)))).....)).))))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_449	Description	PD4S
Aptamer chemistry	DNA	Length	45 nt
GC content	51.1%	Molecular weight	13,842.88 Da
Molarity of 1 μg/μl solution	72.24 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	CGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCG
Applications	Anti-PD-1 aptamer PD4S could be applied as an alternative agent in immunotherapy

Type	Detail	Type	Detail
Uniprot ID	Q15116	Protein name	Programmed cell death protein 1 (Protein PD-1) (hPD-1) (CD antigen CD279)
Gene name(s)	PDCD1 PD1	Organism	Programmed cell death protein 1 (Protein PD-1) (hPD-1) (CD antigen CD279)
Status	reviewed in uniprot database	Length	288
Mass	31,647	Activity regulation	Inhibited by pembrolizumab
Involvement in disease	Systemic lupus erythematosus 2 (SLEB2)	Catalytic activity	N/A
PDB ID(s)	2M2D;3RRQ;4ZQK;5B8C;5GGR;5GGS;5IUS;5JXE;5WT9;6HIG;6J14;6J15;6JBT;6JJP;6K0Y;6R5G;6ROY;6ROZ;6UMT;6UMU;6UMV;6XKR;7BXA;7CGW;7CU5;7E9B;7VUX;
Function	Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self.

No relevant experimental diagram	Interaction ID	454
	Target type	Protein
	Target unique ID	Q15116
	Activity	10.3 nM
	Binding Conditions/Buffer	4.5 g/L glucose and 5 mM MgCl2 in Dulbecco's PBS without calcium and magnesium
	Assay	N/A
	PubMed ID	35019367

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_930	DNA	TTGCACTATGTTTTAGCTAGGGTTCCCTCCGGAGATAGTAAGTGCAA	76.60%
Apta_451	DNA	ATACCAGCTTATTCAATTGAGTAAGAGTGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCAATCT	57.89%
Apta_348	DNA	ACTTGCTCTGATGCGACAACCTTTTGCCCAGATAGTAAGT	57.78%
Apta_76	DNA	CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG	56.67%
Apta_142	DNA	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA	55.56%
Apta_476	DNA	CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG	55.56%
Apta_850	DNA	CACCAGTGTGTTGAGGTTTGAGCACACTGATAGAGTGTCA	55.56%
Apta_694	DNA	ACTCACTATGGAAGAGATGGCGACATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT	55.36%
Apta_754	DNA	GGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGT	54.72%
Apta_489	DNA	GGGGTTAAATATTCCCACATTGCCTGCGCCAGTACAAATAG	53.33%
Apta_663	DNA	CACGCACAGAAGACACCTACAGGGCCAGATCACAATCGGTTAGTGAACTCGTACGGCGCG	53.33%
Apta_722	DNA	CTCTCGGGACGACGGCCCGATCTCAGAGTAGTCGTCCC	53.33%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	53.33%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	53.33%
Apta_1263	DNA	CCCCGCTGCCCGTGTTCCGTCCTCCCTTGCTGTGTGTGCG	53.33%
Apta_910	DNA	TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA	52.73%
Apta_687	DNA	GGGACAGCGGAGGTCTCCCACCCACCGGCCCCTGCGGCACACCAACCTGTATGGATGCG	52.54%
Apta_595	DNA	AGCGAGGGCGGTGTCCAACAGCGGTTTTTTCACGAGGAGGTTGGCGGTGG	52.00%
Apta_573	DNA	CGTACAGTGGGCTATATTGCTGACGTAAGAGCTGCTTTCAATGCATTGGCATAGATGTCA	51.67%
Apta_144	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG	51.11%

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