AptaDB: Result

Interaction ID: 248

Aptamer sequence: GCUUGAAGCUAGCAGUAAGACUAGCGCUACGGAUGGGUGUGCG

Target unique ID: P07975

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_243	HA12-8	Protein	HEMA_INCJH	N/A	20 mM sodium phosphate, pH 7.5, 500 mM NaCl and 20 mM imidazole	24835440

Aptamer Sequence:	GCUUGAAGCUAGCAGUAAGACUAGCGCUACGGAUGGGUGUGCG
The optimal secondary structure in dot-bracket notation:	.......(((((........)))))(((((......))).)).
The centroid secondary structure in dot-bracket notation:	.......(((((........)))))(((((......))).)).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_243	Description	HA12-8
Aptamer chemistry	RNA	Length	43 nt
GC content	55.8%	Molecular weight	14,190.27 Da
Molarity of 1 μg/μl solution	70.47 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	GCUUGAAGCUAGCAGUAAGACUAGCGCUACGGAUGGGUGUGCG
Applications	Suppressed viral infection

Type	Detail	Type	Detail
Uniprot ID	P07975	Protein name	Hemagglutinin-esterase-fusion glycoprotein (HEF) (EC 3.1.1.53) [Cleaved into: Hemagglutinin-esterase-fusion glycoprotein chain 1 (HEF1); Hemagglutinin-esterase-fusion glycoprotein chain 2 (HEF2)]
Gene name(s)	HE	Organism	Hemagglutinin-esterase-fusion glycoprotein (HEF) (EC 3.1.1.53) [Cleaved into: Hemagglutinin-esterase-fusion glycoprotein chain 1 (HEF1); Hemagglutinin-esterase-fusion glycoprotein chain 2 (HEF2)]
Status	reviewed in uniprot database	Length	655
Mass	72,131	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=H2O + N-acetyl-9-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate
PDB ID(s)	1FLC;6WKO;
Function	Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell.

	Interaction ID	248
	Target type	Protein
	Target unique ID	P07975
	Activity	N/A
	Binding Conditions/Buffer	20 mM sodium phosphate, pH 7.5, 500 mM NaCl and 20 mM imidazole
	Assay	The antiviral effect of selected aptamers on the viability of MDCK cells infected by the influenza virus was evaluated using the MTT method. MDCK cells were infected with influenza virus H3N2 at an MOI of 0.1 TCID50 and then treated with the selected RNA aptamers (5 pmol). The highest protective activity was observed by HA12-16, as compared with other selected RNA aptamers
	PubMed ID	24835440

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_241	RNA	GCUUGACGGAGAUCAAGGGCGAGUCUCAUACCAAGUUGAUGGGG	56.82%
Apta_925	RNA	GCUCGGCCAGGUCGCGGGUGGAUGUGAGGG	55.81%
Apta_926	RNA	UCGGCAGCGGUUAGAGAGGUGGUGUGCGGG	55.81%
Apta_242	RNA	GCUUCCAGGAAGAGAAGUGUCAUGUGAUCAGUCCUGGUGGGUGG	54.55%
Apta_244	RNA	GCUUCCGGAGAGAAGGGUCAAAGUUGUGCGGGAGUGUGUUGUGG	54.55%
Apta_402	RNA	GCCUGUAAGGUGGUCGGUGUGGCGAGUGUGUUAGGAGAGAUUGC	54.55%
Apta_712	RNA	GGCGGUGCUCGCCGAAAGGCUCCUGGAUACGUC	53.49%
Apta_270	RNA	CUGUCGAACAUGCUGAGGGUAACGUACUGGCAAGCUCACCUCAGCGUAGCUAa	52.83%
Apta_632	RNA	GACGAGAAGGAGUGCUGGUUAUACUAGCGGUUAGGUCACUCGUC	52.27%
Apta_431	RNA	CAGGCAUGCCUAGCUAAGCAGCCCAUGGCUUAUGCGCGGAAUAUUGGCUUCCGUUC	51.79%
Apta_1	RNA	ACGAAGCUUGAUCCCGUUUGCCGGUCGAUCGCUUCGA	51.16%
Apta_51	RNA	GCCUUAGUAACGUGCUUUGAUGUCGAUUCGACAGGAGGC	51.16%
Apta_93	RNA	CUCCGCUAUCUAACGUAUGAUAGAAUGGAUGACUA	51.16%
Apta_142	DNA	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA	51.16%
Apta_230	RNA	GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG	51.16%
Apta_231	RNA	GGGAGGACGAUGCGGGUGUGGGGCG	51.16%
Apta_365	RNA	GGACCUAUGCAGUAGCCAGUGUGGACUGGGCUGCCCCCCC	51.16%
Apta_374	RNA	GUCCCGUGCGCAAAAGUUCUUAGCGCUAGCAGUCCUAGUUGC	51.16%
Apta_647	RNA	GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC	51.16%
Apta_713	RNA	GGCGGUGCUGGCCGAAAGGCGCUUGGAAACGUC	51.16%

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