AptaDB: Result

Interaction ID: 1015

Aptamer sequence: GCUCGGCCAGGUCGCGGGUGGAUGUGAGGG

Target unique ID: Other-34

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_925	TAFapt2	Other	leukemic AML1-MTG8 fusion protein	81.6 nM	20 mM sodium phosphate (pH 6.5), 2 mM magnesium acetate, 200 mM potassium acetate, 5% glycerol, 0.05% Triton X-100, 5 mM b-mercaptoethanol	32870501

Aptamer Sequence:	GCUCGGCCAGGUCGCGGGUGGAUGUGAGGG
The optimal secondary structure in dot-bracket notation:	((((.(((........))).)).)).....
The centroid secondary structure in dot-bracket notation:	((((.(((........))).)).)).....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_925	Description	TAFapt2
Aptamer chemistry	RNA	Length	30 nt
GC content	73.3%	Molecular weight	9,810.86 Da
Molarity of 1 μg/μl solution	101.93 μM	Number of G-quadruplexes	2
G-Score	19	Function	Targeted therapy
Sequence	GCUCGGCCAGGUCGCGGGUGGAUGUGAGGG
Applications	The aptamer could bind to the AML1-MTG8 fusion protein and dissociate the AML1-MTG8/DNA complex, suggesting that it can inhibit the dominant negative effects of AML1-MTG8 against normal AML1 function and serve as a potential therapeutic agent for leukemia

Type	Detail	Type	Detail
Target ID	Other-34	Name	leukemic AML1-MTG8 fusion protein

No relevant experimental diagram	Interaction ID	1015
	Target type	Other
	Target unique ID	Other-34
	Activity	81.6 nM
	Binding Conditions/Buffer	20 mM sodium phosphate (pH 6.5), 2 mM magnesium acetate, 200 mM potassium acetate, 5% glycerol, 0.05% Triton X-100, 5 mM b-mercaptoethanol
	Assay	N/A
	PubMed ID	32870501

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_926	RNA	UCGGCAGCGGUUAGAGAGGUGGUGUGCGGG	63.33%
Apta_231	RNA	GGGAGGACGAUGCGGGUGUGGGGCG	60.00%
Apta_706	DNA	CCGGCCAAGGGTGGGAGGGAGGGGGCCGG	56.67%
Apta_243	RNA	GCUUGAAGCUAGCAGUAAGACUAGCGCUACGGAUGGGUGUGCG	55.81%
Apta_244	RNA	GCUUCCGGAGAGAAGGGUCAAAGUUGUGCGGGAGUGUGUUGUGG	54.55%
Apta_402	RNA	GCCUGUAAGGUGGUCGGUGUGGCGAGUGUGUUAGGAGAGAUUGC	54.55%
Apta_230	RNA	GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG	54.29%
Apta_10	DNA	GGGGGGGGTGGCTGGGCGGGGTGGG	53.33%
Apta_377	RNA	CUUCGGGAUUUGAGGGUAGAAUGGGACUA	53.33%
Apta_1098	DNA	ATGGCCAGGGGGGAGGGG	53.33%
Apta_1194	DNA	GGGAGAGGTGGGAGCGGGGCCAGGG	53.33%
Apta_218	RNA	AGCCGCGAGGGGAGGGAUAGGGUAGGGCGCGGCU	52.94%
Apta_98	RNA	CGAUGCGGUCUCAUGCGUCGAGUGUGAGUUUACCUUCG	52.63%
Apta_1189	DNA	GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG	52.50%
Apta_703	RNA	GGGCGGAUGAGACGCUUGGCGUGUGCUGUGGAGAGUCAUCCG	52.38%
Apta_241	RNA	GCUUGACGGAGAUCAAGGGCGAGUCUCAUACCAAGUUGAUGGGG	52.27%
Apta_9	DNA	TGAGGGGGCGGCGGGGCCGGGCAGG	50.00%
Apta_14	DNA	AGCGGGGGGGGCTGGGCTGGGTGGG	50.00%
Apta_21	DNA	TGTGGGGGAGGACGGGCGGGGATGC	50.00%
Apta_242	RNA	GCUUCCAGGAAGAGAAGUGUCAUGUGAUCAGUCCUGGUGGGUGG	50.00%

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