Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 83
Aptamer sequence: GGCTTTACACTTTATGCTTCCGAAACAACGCAGAAAGTCTTTACAACATTACATAACAATTTCACACAGGAAACAG
Target unique ID: P00740
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_82 | BnFIX1 | Protein | FA9_HUMAN | 20.4 nM | 20 mM HEPES pH 7.5, 150 mM NaCl, 6 mM KCl, 2 mM MgCl2, room temperature | 31961667 |
Structure information of aptamer
| Aptamer Sequence: | GGCTTTACACTTTATGCTTCCGAAACAACGCAGAAAGTCTTTACAACATTACATAACAATTTCACACAGGAAACAG |
| The optimal secondary structure in dot-bracket notation: | ........(((((.(((............))).))))).....................((((......))))... |
| The centroid secondary structure in dot-bracket notation: | ........(((((.(((............))).)))))......................(((......))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_82 | Description | BnFIX1 |
| Aptamer chemistry | DNA | Length | 76 nt |
| GC content | 36.8% | Molecular weight | 23,537.98 Da |
| Molarity of 1 μg/μl solution | 42.48 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | GGCTTTACACTTTATGCTTCCGAAACAACGCAGAAAGTCTTTACAACATTACATAACAATTTCACACAGGAAACAG | ||
| Applications | Inhibition of catalytic activity of target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P00740 | Protein name | Coagulation factor IX (EC 3.4.21.22) (Christmas factor) (Plasma thromboplastin component) (PTC) [Cleaved into: Coagulation factor IXa light chain; Coagulation factor IXa heavy chain] |
| Gene name(s) | F9 | Organism | Coagulation factor IX (EC 3.4.21.22) (Christmas factor) (Plasma thromboplastin component) (PTC) [Cleaved into: Coagulation factor IXa light chain; Coagulation factor IXa heavy chain] |
| Status | reviewed in uniprot database | Length | 461 |
| Mass | 51,778 | Activity regulation | N/A |
| Involvement in disease | Hemophilia B (HEMB) | Catalytic activity | Reaction=Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa |
| PDB ID(s) | 1CFH;1CFI;1EDM;1IXA;1MGX;1NL0;1RFN;2WPH;2WPI;2WPJ;2WPK;2WPL;2WPM;3KCG;3LC3;3LC5;4WM0;4WMA;4WMB;4WMI;4WMK;4WN2;4WNH;4YZU;4Z0K;4ZAE;5EGM;5F84;5F85;5F86;5JB8;5JB9;5JBA;5JBB;5JBC;5TNO;5TNT;5VYG;6MV4;6RFK;6X5J;6X5L;6X5P;7AHV; |
 |
Function | Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. |
Activity data
 |
Interaction ID | 83 |
| Target type | Protein | |
| Target unique ID | P00740 | |
| Activity | 20.4 nM | |
| Binding Conditions/Buffer | 20 mM HEPES pH 7.5, 150 mM NaCl, 6 mM KCl, 2 mM MgCl2, room temperature |
|
| Assay | Binding of BnFIX1, BnFIX2, BnFIX3, and their natural counterparts to factor IXa. Data are average ± SD of three independent determinations |
|
| PubMed ID | 31961667 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_83 | DNA | GGCTTTACACTTTATGCTTCCGTAAAAACACAAACACACGCACATACATAAAATAACAATTTCACACAGGAAACAG | |
| Apta_246 | DNA | GGCTTTACACTTTATGCTTCCGGCCCGCGGAAATGATAACGCGTATGGTGGTATAACAATTTCACACAGGAAACAG | |
| Apta_247 | DNA | GGCTTTACACTTTATGCTTCCGTACCAGAACATATCGAGTGATCCTGGGAGGATAACAATTTCACACAGGAAACAG | |
| Apta_84 | DNA | GGCTTTACACTTTATGCTTCCGTTGCAATTTTAATGGGAGGTACATGGCGGAATAACAATTTCACACAGGAAACAG | |
| Apta_248 | DNA | GGCTTTACACTTTATGCTTCCGGCCGAGGATTTTCATACCCCGCACGAACGCATAACAATTTCACACAGGAAACAG | |
| Apta_582 | DNA | GTACCAGCTTATTCAATTGGGCGAAGGAACATACGGCAGTTTATGGCCGCTATCGAGATAGACTATCATCTCAACGTCTTCTAGATAGTATGTTCATCAG | |
| Apta_934 | DNA | GACGCTTACTCAGGTGTGACTCGTGAACCCACCTCAGGGCATCTTACATTTCTTCTAAGTTGTTACCATGTTTCGAAGGACGCAGATGAAGTCTC | |
| Apta_1130 | DNA | GCCGAGATTGCACTTACTATCTATTAGGAATAAGGTATGAATAGAGATGTATAAGTTGTGTGGACGGTGTAATTGAATAAGCTGGTATGCGC | |
| Apta_1274 | DNA | GACTTCGTCGTCTTTAGACTAGCAGTTAGGGTTTACGATTGTGGAGTTAGCCCCCAGGG | |
| Apta_1031 | DNA | GGAGACCGCTTAAGCTTAAAGGAGCGGTTACGAATGCGATGACTTAGGTACCATTGCACTACGGTACCTAAACAGTTCCTTTGGATCCGAATTCGCCGC | |
| Apta_935 | DNA | GACGCTTACTCAGGTGTGACTCGTCCGTCGGTTCGCCTTGCGAGTGCACGCCGCCAGCCGTTACATAAAGTCTGCCCACTCGAAGGACGCAGATGAAGTCTC | |
| Apta_458 | DNA | AGCTCCAGAAGATAAATTACAGGGAGAGTAAACTTTGCAAACACAACAATACCATTCCGGAACGTTCTTAACACAACTAGGATACTATGACCCC | |
| Apta_321 | DNA | AACCGCCCAAATCCCTAAGAGTCGGACTGCAACCTATGCTATCGTTGATGTCTGTCCAAGCAACACAGACACACTACACACGCACA | |
| Apta_792 | DNA | ATACCAGCTTATTCAATTCCCAAATTGCCACCACTTACAGCATGATAACATACTACATCTTTTCATCAAGATAGTAAGTGCAATCT | |
| Apta_403 | DNA | GTCTTGACTAGTTACGCCCCCAAGAACCCGCTTGCCGTGGTGACGTTGATCATGTCGTTTGGTCATTCAGTTGGCGCCTC | |
| Apta_316 | DNA | CCGCCCAAATCCCTAAGAGAAGACTGTAATGACATCAAACCAGACACACTACACACGCA | |
| Apta_502 | DNA | GGGAGACAAGAATAAACGCTCAAATGGACTTTTGAATGTGGCAACAAATTCGACAGGAGGCTCACAACAGGC | |
| Apta_790 | DNA | GGGAGACAAGAATAAACGCTCAAGAAGTGAAAATGACAGAACACAACATTCGACAGGAGGCTCACAACAGGC | |
| Apta_994 | DNA | GCAATGGTACGGTACTTCCGGACAGTGCTGAAAACTGTGACCCCCCAAAAGTGCACGCTACTTTGCTAA | |
| Apta_1108 | DNA | GTGCTTAGGTGCAGAACGCACACACGCTCGGTATGACGTGTTCGCTACGGTATAATTGCAGGCGCGAGCACAACAG |
DEV TOOL
 |
 |
 |
 |