Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1028
Aptamer sequence: AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA
Target unique ID: Other-38
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_942 | QA-12 | Other | Soft-shelled turtle iridovirus (STIV) | 80.7 nM | 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris-HCl (pH 7.5) | 26419355 |
Structure information of aptamer
| Aptamer Sequence: | AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA |
| The optimal secondary structure in dot-bracket notation: | ...((.((..((((.((...(((....)))...)).))))..)).))... |
| The centroid secondary structure in dot-bracket notation: | ......((..((((.((...(((....)))...)).))))..))...... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_942 | Description | QA-12 |
| Aptamer chemistry | DNA | Length | 50 nt |
| GC content | 64.0% | Molecular weight | 15,811.08 Da |
| Molarity of 1 μg/μl solution | 63.25 μM | Number of G-quadruplexes | 3 |
| G-Score | 49 | Function | Targeted therapy |
| Sequence | AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA | ||
| Applications | Inhibited STIV infection in vitro and in vivo, with aptamer QA-36 demonstrating the greatest protective effect against STIV and inhibiting STIV infection in a dose-dependent manne | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-38 | Name | Soft-shelled turtle iridovirus (STIV) |
Activity data
 |
Interaction ID | 1028 |
| Target type | Other | |
| Target unique ID | Other-38 | |
| Activity | 80.7 nM | |
| Binding Conditions/Buffer | 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris-HCl (pH 7.5) |
|
| Assay | Affinity of STIV-selected ssDNA aptamer interactions by ELISA. STIV was incubated with increasing concentrations of 5′-biotinylated aptamer. After addition of streptavidin-HRP, the amount of STIV-aptamer complex was calculated and graphed as a function of aptamer concentration. The graph was fit to the equation Y = Bmax*X/(Kd + X) using SigmaPlot software. Results for each aptamer was presented as the mean ± SD of three independent experiments |
|
| PubMed ID | 26419355 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1014 | DNA | TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT | |
| Apta_263 | DNA | AGAGGGGAGGGTCGGGTATCGGCGTGTTCGGGGGATCTGC | |
| Apta_746 | DNA | AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT | |
| Apta_46 | DNA | CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT | |
| Apta_255 | DNA | TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT | |
| Apta_744 | DNA | GTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTG | |
| Apta_750 | DNA | ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT | |
| Apta_617 | DNA | CAATGGGGTCGGGCGGGCCGGGTGTCATGGTGGACGGAGATGGGACGTAGAGGGCGGT | |
| Apta_411 | DNA | AGACGTAAGTTAATTGGACTTGGTCGTGTGCGGCACAGCGATTGAAAT | |
| Apta_565 | DNA | GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG | |
| Apta_862 | DNA | TGTGCCAAAGAGAGTGGTGGGGGGGTGGGCGGAACTCGCG | |
| Apta_1026 | DNA | GGGGGGTGTATCGTTGACGAGTTGCGCGTGCGTCTCGTG | |
| Apta_1188 | DNA | CGGGCGGGGCGTGGGGTGTTGGAGTGGAGGGCGGGGCGGC | |
| Apta_1189 | DNA | GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG | |
| Apta_1229 | DNA | CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG | |
| Apta_1090 | DNA | ATCCAGAGTGACGCAGCAGGGCTTGGGTTGGGAATAAGGATGTGGGAGGCGGCGAACATGGACACGGTGGCTTAGT | |
| Apta_656 | DNA | GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_76 | DNA | CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG | |
| Apta_832 | DNA | CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA | |
| Apta_667 | DNA | GCGGCGGTACCAGGTCAAAGGTGGGTGAGGGGACGCCAAGAGTCCCCGCGGTTACATAGAG |
DEV TOOL
 |
 |
 |
 |