Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 409
Aptamer sequence: GTCTTGACTAGTTACGCCCCCAAGAACCCGCTTGCCGTGGTGACGTTGATCATGTCGTTTGGTCATTCAGTTGGCGCCTC
Target unique ID: P48551
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_403 | IFNα-1 aptamer | Protein | INAR2_HUMAN | 4.52 nM | 10 mM Tris pH 7.5, 10 mM MgCl2, 50 mM KCl, 25 mM NaCl | 33063508 |
Structure information of aptamer
| Aptamer Sequence: | GTCTTGACTAGTTACGCCCCCAAGAACCCGCTTGCCGTGGTGACGTTGATCATGTCGTTTGGTCATTCAGTTGGCGCCTC |
| The optimal secondary structure in dot-bracket notation: | .(((((..............)))))....((..((((...(((...((((((.......)))))).)))..))))))... |
| The centroid secondary structure in dot-bracket notation: | ..((((..............))))......................((((((.......))))))............... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_403 | Description | IFNα-1 aptamer |
| Aptamer chemistry | DNA | Length | 80 nt |
| GC content | 55.0% | Molecular weight | 24,561.66 Da |
| Molarity of 1 μg/μl solution | 40.71 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | GTCTTGACTAGTTACGCCCCCAAGAACCCGCTTGCCGTGGTGACGTTGATCATGTCGTTTGGTCATTCAGTTGGCGCCTC | ||
| Applications | Designing of aptamers similar to the IFNα-3 aptamer provides a novel approach to assess other inflammatory protein(s) in patients before, during, and after completion of treatment and would denote clinical improvement in successfully treated patients | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P48551 | Protein name | Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha binding protein) (IFN-alpha/beta receptor 2) (Interferon alpha binding protein) (Type I interferon receptor 2) |
| Gene name(s) | IFNAR2 IFNABR IFNARB | Organism | Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha binding protein) (IFN-alpha/beta receptor 2) (Interferon alpha binding protein) (Type I interferon receptor 2) |
| Status | reviewed in uniprot database | Length | 515 |
| Mass | 57,759 | Activity regulation | N/A |
| Involvement in disease | Immunodeficiency 45 (IMD45) | Catalytic activity | N/A |
| PDB ID(s) | 1N6U;1N6V;2HYM;2KZ1;2LAG;3S8W;3S9D;3SE3;3SE4; |
 |
Function | Associates with IFNAR1 to form the plasma membrane receptor in the type I interferon signaling pathway. |
Activity data
 |
Interaction ID | 409 |
| Target type | Protein | |
| Target unique ID | P48551 | |
| Activity | 4.52 nM | |
| Binding Conditions/Buffer | 10 mM Tris pH 7.5, 10 mM MgCl2, 50 mM KCl, 25 mM NaCl |
|
| Assay | Binding affinity of selected aptamers. The dissociation constant (Kd) curve derived through nonlinear regression represents the binding affinity of (C) IFNα-1 and (D) IFNα-3 aptamers to 250 ng of the IFN-α protein |
|
| PubMed ID | 33063508 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_404 | DNA | GTCTTGACTAGTTACGCCCACAAGAACCTGCTTGCCATGGTGACGCCGATCATGCTTTTTGGTCATTCAGTTGGCGCCTC | |
| Apta_738 | DNA | ATCGTCTGCTCCGTCCAATACCTGCATATACACTTTGCATGTGGTTTGGTGTGAGGTCGTGC | |
| Apta_1071 | DNA | TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT | |
| Apta_1173 | DNA | TTGACTTGCCACTGACTACCCCAGTATCTCTTCAACGCTATGGTGCATGTACTACGTATTGAAGTCAGTCGGTCGTCATC | |
| Apta_627 | DNA | ATACCAGCTTATTCAATTATGCGGGGATGAGGCTTGATCTGTGGCTCTGATCCATGATCAGTCGTCTTACGTGCGGTCCAGATAGTAAGTGCAATCT | |
| Apta_804 | DNA | GCGGGATCCTATGACGCATTGACCCACAACACACTACTGTCGCTCGGTTCGAACTTCGTGCGACTGTTCCCTATAGTGAGTCGTATTAGAATTCCGC | |
| Apta_627 | DNA | ATACCAGCTTATTCAATTATGCGGGGATGAGGCTTGATCTGTGGCTCTGATCCATGATCAGTCGTCTTACGTGCGGTCCAGATAGTAAGTGCAATCT | |
| Apta_127 | DNA | GGTATTGAGGGTCGCATCCACTGGTCGTTGTTGTCTGTTGTCTGTTATGTTGTTTCGTGATGGCTCTAACTCTCCTCT | |
| Apta_570 | DNA | GGCAAGATGTGCCCAGAACAGTTGCTTGTATGGTGGGGAGCGTCCATATTGGCTTAAAC | |
| Apta_600 | DNA | GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC | |
| Apta_653 | DNA | CCTGCACCTCGTCCAGCATCCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGGTG | |
| Apta_737 | DNA | ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC | |
| Apta_997 | DNA | ATAGGAGTCGACCGACCAGACCCCGCCTCTCCTTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC | |
| Apta_1003 | DNA | ATAGGAGTCGACCGACCAGACCCCGCCTCTCCTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC | |
| Apta_600 | DNA | GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC | |
| Apta_281 | DNA | CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT | |
| Apta_704 | DNA | GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCGAGCATGGAGTCGGATCCTCTA | |
| Apta_490 | DNA | GGGAGACAAGAATAAACGCTCAAGGGCGTGGTGGGTGGGGTACTAATAATGTGCGTTTGTTCGACAGGAGGCTCACAACAGGC | |
| Apta_1135 | DNA | GCCGAGATTGCACTTACTATCTCATCAATAAAGATTATAGGGTGAAGGTGATAGTAGGTGGTATATAGGGTAATTGAATAAGCTGGTATGCGC | |
| Apta_78 | DNA | ATCTCGACTAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACAATACTCGT |
DEV TOOL
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