Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 244
Aptamer sequence: CCCAGATTACTGTGGAGTGGTTGTCTGCGAATCCTTCGTCCACCCAATAG
Target unique ID: P05106
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_239 | β3-1 | Protein | ITB3_HUMAN | 6.5 nM | PBS supplemented with 0.05% Tween-20 and 0.1% BSA | 22624874 |
Structure information of aptamer
| Aptamer Sequence: | CCCAGATTACTGTGGAGTGGTTGTCTGCGAATCCTTCGTCCACCCAATAG |
| The optimal secondary structure in dot-bracket notation: | .........((((((.((((......(((((...))))))))))).)))) |
| The centroid secondary structure in dot-bracket notation: | .........((((((.((((......(((((...)))))))))))).))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_239 | Description | β3-1 |
| Aptamer chemistry | DNA | Length | 50 nt |
| GC content | 52.0% | Molecular weight | 15,567.77 Da |
| Molarity of 1 μg/μl solution | 64.24 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | CCCAGATTACTGTGGAGTGGTTGTCTGCGAATCCTTCGTCCACCCAATAG | ||
| Applications | Recognize the αV and β3 subunits | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P05106 | Protein name | Integrin beta-3 (Platelet membrane glycoprotein IIIa) (GPIIIa) (CD antigen CD61) |
| Gene name(s) | ITGB3 GP3A | Organism | Integrin beta-3 (Platelet membrane glycoprotein IIIa) (GPIIIa) (CD antigen CD61) |
| Status | reviewed in uniprot database | Length | 788 |
| Mass | 87,058 | Activity regulation | N/A |
| Involvement in disease | Glanzmann thrombasthenia 2 (GT2) | Catalytic activity | N/A |
| PDB ID(s) | 1JV2;1KUP;1KUZ;1L5G;1M1X;1M8O;1MIZ;1MK7;1MK9;1S4X;1TYE;1U8C;2K9J;2KNC;2KV9;2L1C;2L91;2LJD;2LJE;2LJF;2MTP;2N9Y;2Q6W;2RMZ;2RN0;2VC2;2VDK;2VDL;2VDM;2VDN;2VDO;2VDP;2VDQ;2VDR;3FCS;3FCU;3IJE;3NID;3NIF;3NIG;3T3M;3T3P;3ZDX;3ZDY;3ZDZ;3ZE0;3ZE1;3ZE2;4CAK;4G1E;4G1M;4MMX;4MMY;4MMZ;4O02;4Z7N;4Z7O;4Z7Q;5HDB;6AVQ;6AVR;6AVU;6BXB;6BXF;6BXJ;6CKB;6MK0;6MSL;6MSU;6NAJ;6V4P;7KN0;7LA4; |
 |
Function | Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. |
Activity data
 |
Interaction ID | 244 |
| Target type | Protein | |
| Target unique ID | P05106 | |
| Activity | 6.5 nM | |
| Binding Conditions/Buffer | PBS supplemented with 0.05% Tween-20 and 0.1% BSA |
|
| Assay | Affiinity measurement of aptamers αV-1 and β3-1 |
|
| PubMed ID | 22624874 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1278 | DNA | CCATGATTCAACTTTACTGGTCTTGTCTTGGCTAGTCGTGTGTCATTCCCTAAGG | |
| Apta_861 | DNA | CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG | |
| Apta_1066 | DNA | CCTACGTTGGAGTGGGGTTTGCGCAGGCCGTTCTTTCCAA | |
| Apta_1284 | DNA | ACCTGTTATGTTTGAGAGAGGGTAGTCAATAGTGTTATGCCCGCTTCCCCCCTTTGGGGG | |
| Apta_310 | DNA | GCGCCAGCTTTGCTGATGGGTGGCCACCCTTGCCCTGGGTTTGAATTTCGATCCTATCG | |
| Apta_42 | DNA | GCTGGTCTCTGCGGGTTGTTGCGCCGCGGCACCCTTGGCA | |
| Apta_1117 | DNA | CGTACGGAATTCGCTAGCGGACTGGAGTCTAGACCGGGTAGCTGTGGTGGATCCGAGCTCCACGTG | |
| Apta_405 | DNA | TCTAGCCCACTGCATAGAGGTAGCCACGTATGTGGGTGTTACCCATTCGTAGCTACCGGTG | |
| Apta_181 | DNA | CCGTGTAAGGTTTCAGTGGCTTATCCGAAGTAACCCGAGA | |
| Apta_750 | DNA | ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT | |
| Apta_829 | DNA | CCCTCCGGGGGGGTCATCGGGATACCTGGTAAGGATA | |
| Apta_987 | DNA | GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC | |
| Apta_1061 | DNA | TATTTATTGAATGCTGTAATTTCGCCACGCGAAAGTAGG | |
| Apta_1138 | DNA | GCAGTGTGGGCGAATCTATGCGTACCGTTCGATATCGTG | |
| Apta_1254 | DNA | GGCAGTGTGGGCGAATCTATGCGTACCGTTCGATATCGTG | |
| Apta_461 | DNA | CGCTCGGCTTCACGAGATTCGTGTCGTTGTGTCCTGTACCCGCCTTGACCAGTCACTCTAGAGCATCCGGACTG | |
| Apta_1277 | DNA | TCCGTGGTACGGTTACTCGTTCCTTATGTATGTTGCTACAATGGATTGCGTCCCCAGGG | |
| Apta_878 | DNA | TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGTGGGTGGGCTTCGCACACACGGACTTAC | |
| Apta_3 | DNA | CCTGCCTTTGGCAGCCGGGCGAAGGCAACCCGACACCGACAGC | |
| Apta_33 | DNA | GCGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCGCCGCGAAGCG |
DEV TOOL
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