Aptamer-target interaction descriptor
Aptamer-target interaction information
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1084 | Tac-B2 | Protein | TACD2_HUMAN | 89 nM | 1 mM MgCl2 in PBS Buffer, Binding-20 mM Tris-HCl buffer [pH 7.4], 10 mM NaCl, 1 mM MgCl2, and 0.05% Tween 20 | 34204006 |
Structure information of aptamer
| Aptamer Sequence: | TTCCCTCCTCTGTTGTTCCCCCCTCCTCTC |
| The optimal secondary structure in dot-bracket notation: | .............................. |
| The centroid secondary structure in dot-bracket notation: | .............................. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1084 | Description | Tac-B2 |
| Aptamer chemistry | DNA | Length | 30 nt |
| GC content | 60.0% | Molecular weight | 8,873.68 Da |
| Molarity of 1 μg/μl solution | 112.69 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | TTCCCTCCTCTGTTGTTCCCCCCTCCTCTC | ||
| Applications | Capacity for internalization by cells | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P09758 | Protein name | Tumor-associated calcium signal transducer 2 (Cell surface glycoprotein Trop-2) (Membrane component chromosome 1 surface marker 1) (Pancreatic carcinoma marker protein GA733-1) |
| Gene name(s) | TACSTD2 GA733-1 M1S1 TROP2 | Organism | Tumor-associated calcium signal transducer 2 (Cell surface glycoprotein Trop-2) (Membrane component chromosome 1 surface marker 1) (Pancreatic carcinoma marker protein GA733-1) |
| Status | reviewed in uniprot database | Length | 323 |
| Mass | 35,709 | Activity regulation | N/A |
| Involvement in disease | Corneal dystrophy, gelatinous drop-like (GDLD) | Catalytic activity | N/A |
| PDB ID(s) | 2MAE;2MVK;2MVL;7E5M;7E5N;7PEE; |
 |
Function | May function as a growth factor receptor. |
Activity data
 |
Interaction ID | 1138 |
| Target type | Protein | |
| Target unique ID | P09758 | |
| Activity | 89 nM | |
| Binding Conditions/Buffer | 1 mM MgCl2 in PBS Buffer, Binding-20 mM Tris-HCl buffer [pH 7.4], 10 mM NaCl, 1 mM MgCl2, and 0.05% Tween 20 |
|
| Assay | Evaluation of the internalization of each aptamer obtained via CE-SELEX and hybrid-type SELEX |
|
| PubMed ID | 34204006 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_251 | DNA | GCCCTACATTCTGTGCGACTCCCCCCCCCGC | |
| Apta_252 | DNA | CCCTTACCTACTCTGCTCCCCCCCCCCCTCGGCTGC | |
| Apta_877 | DNA | GTGCGAAGCGCCTCCACTGTACATCCACTCCTTCTGCC | |
| Apta_286 | DNA | TACTCCGTCACTTTAGTGGATATAACAACCTCCCTATGGG | |
| Apta_872 | DNA | ACCGCCTTCCACCGTTCTCCACCACCCCTCAAACAACCCT | |
| Apta_1192 | DNA | CTTTCTGTCCTTCCGTCACATCCCACGCATTCTCCACAT | |
| Apta_504 | DNA | TTGCTCCTTATAGAGCCGTCCGAGC | |
| Apta_1269 | DNA | TAGCACACAACCCTTTGATTGGGATATCTCATCCCTCG | |
| Apta_167 | DNA | TGCTCCTTGGCCTTAGCCCTGGAACCATCAATCCTCTTCG | |
| Apta_604 | DNA | TCCTGACCTTAGCCTATGTTTTCTCTACCGGCTATGTTAC | |
| Apta_1263 | DNA | CCCCGCTGCCCGTGTTCCGTCCTCCCTTGCTGTGTGTGCG | |
| Apta_937 | DNA | TTGCCCACAGATCTGTGGAAGCCGAACCGCGTGCTAGTCGTG | |
| Apta_939 | DNA | TTGCCCACCGTACTGTGCAGGTCGAACTACAGGCACGTCGTG | |
| Apta_253 | DNA | CCCTGTTCGCTATCCCCCATCCCCCGATTGC | |
| Apta_42 | DNA | GCTGGTCTCTGCGGGTTGTTGCGCCGCGGCACCCTTGGCA | |
| Apta_238 | DNA | CACACATTCCCGTCCTCGATACGTCTAGGCTTAGTGCCACTTGCTTAATC | |
| Apta_283 | DNA | ACTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGA | |
| Apta_472 | DNA | CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA | |
| Apta_938 | DNA | TTGCCCACCTCCCTGTGCGGGCCACAGAGCAGCAGTGTCGTG | |
| Apta_966 | DNA | GGCCTGGTCTGTTAGCCGGGTAGCAGCCCCGGCACCTATT |
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