Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 878
Aptamer sequence: ATCCAGAGTGACGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGACACGGTGGCTTAGT
Target unique ID: HB-8065
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_794 | ZL-7 | Cell | HCC cell (HepG2/MDR) | 88.31 nM | DPBS with glucose (4.5 mg/mL), MgCl2 (5 mM), BSA (1 mg/mL), and yeast tRNA (0.1 mg/mL)) at 37 °C in an orbital shaker for 50 min | 34779207 |
Structure information of aptamer
| Aptamer Sequence: | ATCCAGAGTGACGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGACACGGTGGCTTAGT |
| The optimal secondary structure in dot-bracket notation: | .....((((.((..((((((....(((......)))..))))))......(((....)))...)).))))... |
| The centroid secondary structure in dot-bracket notation: | ...............(((((....(((......)))..))))).............................. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_794 | Description | ZL-7 |
| Aptamer chemistry | DNA | Length | 73 nt |
| GC content | 50.7% | Molecular weight | 22,880.65 Da |
| Molarity of 1 μg/μl solution | 43.71 μM | Number of G-quadruplexes | 2 |
| G-Score | 18 | Function | Targeted therapy |
| Sequence | ATCCAGAGTGACGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGACACGGTGGCTTAGT | ||
| Applications | Precise diagnosis and target theranostics of multidrug-resistant HCC | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | HB-8065 | Cell name | HCC cell (HepG2/MDR) |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | N/A |
| Morphology | epithelial-like | Tissue | Liver |
| Disease | Carcinoma; Hepatocellular | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | Alpha-fetoprotein (AFP, alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin) |
| Cell description | Hep G2 [HEPG2] is a cell line exhibiting epithelial-like morphology that was isolated from a hepatocellular carcinoma of a 15-year-old, White, male youth with liver cancer. The cell line was deposited by the Wistar Institute and is a suitable transfection host. Expression markers include insulin; insulin-like growth factor II (IGF II). |
|
|
| Application(s) | 3D cell culture; Cancer research; High-throughput screening; Toxicology | ||
Activity data
 |
Interaction ID | 878 |
| Target type | Cell | |
| Target unique ID | HB-8065 | |
| Activity | 88.31 nM | |
| Binding Conditions/Buffer | DPBS with glucose (4.5 mg/mL), MgCl2 (5 mM), BSA (1 mg/mL), and yeast tRNA (0.1 mg/mL)) at 37 °C in an orbital shaker for 50 min |
|
| Assay | Quantitative analysis of the apparent dissociation constant of the ZL-7 aptamer on target HepG2/MDR cells by measuring the median fluorescence intensity after incubating with various concentrations of the FITC-labeled aptamer |
|
| PubMed ID | 34779207 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_785 | DNA | ATCCAGAGTGACGCAGCATGAATCTTTTCTTTTGGTTTTGATATTTATAGTTGGTGAATGGACACGGTGGCTTAGT | |
| Apta_1090 | DNA | ATCCAGAGTGACGCAGCAGGGCTTGGGTTGGGAATAAGGATGTGGGAGGCGGCGAACATGGACACGGTGGCTTAGT | |
| Apta_923 | DNA | ATCCAGAGTGACGCAGCATCGACCTCTGTGCGAGCGGGTTGCATATATATTTTGCATGGACACGGTGGCTTAGT | |
| Apta_922 | DNA | ATCCAGAGTGACGCAGCACACCGTTTGTATTCTGCATTGTTTTGCATTCTACATGGACACGGTGGCTTAGT | |
| Apta_1089 | DNA | ATCCAGAGTGACGCAGCAATTACCGATGGCTTGTTTGTAATGTAGGGTTCCGTCGGATTGGACACGGTGGCTTAGT | |
| Apta_921 | DNA | ATCCAGAGTGACGCAGCACGAGCCAGACATCTCACACCTGTTGCATATACATTTTGCATGGACACGGTGGCTTAGT | |
| Apta_1088 | DNA | ATCCAGAGTGACGCAGCATCGAGTGGCTTGTTTGTAATGTAGGGTTCCGGTCGTGGGTTGGACACGGTGGCTTAGT | |
| Apta_401 | DNA | ATCCAGAGTGACGCAGCAGATAAGTAGGTATCCGTTTGAAAAACTTTTCTGACCGTCCGACTATGGACACGGTGGCTTAGT | |
| Apta_786 | DNA | ATCCAGAGTGACGCAGCATTTGTATCCTATTTTGTTTATGTAATTGTCGTTGATCATGTGGACACGGTGGCTTAGT | |
| Apta_725 | DNA | ATCCAGAGTGACGCAGCATGCTCATCCACGATTCTGGCGAATTTAGTGCCTGTCTTTTTCTCTTGGACACGGTGGCTTAGT | |
| Apta_784 | DNA | ATCCAGAGTGACGCAGCATTTTGACGCTTTATCCTTTTCTTATGGCGGGATAGTTTCGTGGACACGGTGGCTTAGT | |
| Apta_920 | DNA | ATCCAGAGTGACGCAGCACGGCACTCACTCTTTGTTAAGTGGTCTGCTTCTTAACCTTCATCGACACGGTGGCTTA | |
| Apta_795 | DNA | CGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGA | |
| Apta_787 | DNA | ATCCAGAGTGACGCAGCATTTGTTTAATATGTTTTTTAATTCCCCTTGTGGTGTGTTGTGGACACGGTGGCTTAGT | |
| Apta_1293 | DNA | ATCCAGAGTGACGCAGCATGCCCTAGTTACTACTACTCTTTTTAGCAAACGCCCTCGCTTTGGACACGGTGGCTTAGT | |
| Apta_288 | DNA | ATCCAGAGTGACGCAGCATTTCATCGGGTCCAAAAGGGGCTGCTCGGGATTGCGGATATGGACACGT | |
| Apta_1099 | DNA | ATCCATTGCCACTGACTACCAGCCGGGTGGGTGGGGGGGTTTGCTGGTATCGCATTGTGCGAAGTCAGTCGGTCGTTAGT | |
| Apta_812 | DNA | ATAGGAGTCACGACGACCAGAATAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAGTATGTGCGTCTACCTCTTGACTAAT | |
| Apta_988 | DNA | AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG | |
| Apta_887 | DNA | ACGCTCGGATGCCACTACAGGGGGGTGGTCCTGAGGGTGGTGTGGTTGGTTTGGTTTCCTCATGGACGTGCTGGTGAC |
DEV TOOL
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