Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 861
Aptamer sequence: AGTGGTCGAACTACACATCCTTGAACTGCGGAATTATCTAC
Target unique ID: CRL-5800
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_776 | ADE1 | Cell | Lung Adenocarcinoma (H23) | 45 nM | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes | 23082117 |
Structure information of aptamer
| Aptamer Sequence: | AGTGGTCGAACTACACATCCTTGAACTGCGGAATTATCTAC |
| The optimal secondary structure in dot-bracket notation: | .(((((...)))))...(((.........)))......... |
| The centroid secondary structure in dot-bracket notation: | .(((((...)))))...(((.........)))......... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_776 | Description | ADE1 |
| Aptamer chemistry | DNA | Length | 41 nt |
| GC content | 43.9% | Molecular weight | 12,568.11 Da |
| Molarity of 1 μg/μl solution | 79.57 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target detection |
| Sequence | AGTGGTCGAACTACACATCCTTGAACTGCGGAATTATCTAC | ||
| Applications | Early detection, Targeted therapy, and guided surgery of glioblastoma leading to effective treatment of glioblastoma | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CRL-5800 | Cell name | Lung Adenocarcinoma (H23) |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | N/A |
| Morphology | epithelial | Tissue | Lung |
| Disease | Adenocarcinoma; Non-small cell lung cancer | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | Myc+; src+; abl+; erb+; ras+; sis- |
| Cell description | N/A |
|
|
| Application(s) | 3D cell culture; Cancer research; High-throughput screening; Toxicology | ||
Activity data
|
No relevant experimental diagram |
Interaction ID | 859 |
| Target type | Cell | |
| Target unique ID | CRL-5800 | |
| Activity | 70 nM | |
| Binding Conditions/Buffer | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes |
|
| Assay | N/A |
|
| PubMed ID | 23082117 | |
|
No relevant experimental diagram |
Interaction ID | 861 |
| Target type | Cell | |
| Target unique ID | CRL-5800 | |
| Activity | 45 nM | |
| Binding Conditions/Buffer | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes |
|
| Assay | N/A |
|
| PubMed ID | 23082117 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_513 | DNA | TAGGGAAAACCAACCGCCCAGAACTGCAGCATAAC | |
| Apta_876 | DNA | GTTGCGAAAGACAACGAATGCTTTGCCTGCCATAATTTGC | |
| Apta_961 | DNA | CTAGTGGGGACCTTCCAGGGTAACTGCTCGCATTCTCCAT | |
| Apta_971 | DNA | TAGTCTAGGAACGGTACGATCGTATTCCAAGGACTTTGCTGGTGGTATCC | |
| Apta_592 | DNA | CTCTCGGGACGACCACTACGCATAGTTTCTATCGCCAGGAAGGGTCGTCCC | |
| Apta_335 | DNA | ACCGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTG | |
| Apta_973 | DNA | TATGTAATGTTGACGTAATTTATCTCTGTGAGAATAAGCAGGTGGTATCC | |
| Apta_974 | DNA | CATGGTGTCAATACTATACCTTTTGCCGATGGTAACAGTTGGTGGTATCC | |
| Apta_883 | DNA | CGTACCGGCCAGTGATTACGACGAGACGAGCTTATGCGTATTGATGCCTAACTATCTACA | |
| Apta_48 | DNA | ACTGGGCAGATTAACGGTCCTATATTTTCGGTTCTGGAAA | |
| Apta_220 | DNA | ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT | |
| Apta_358 | DNA | GGGAATCGCCACCCGACGCAGGGTTTCCC | |
| Apta_360 | DNA | GGGAACGCATACGCCGCAGTATTTCCC | |
| Apta_361 | DNA | GGGAACGCCCGTATTGCTCGGTTCCC | |
| Apta_362 | DNA | GGGAACGCACCGATCGCAGGTTTCCC | |
| Apta_463 | DNA | GTTCTCATGCACACTTATAGCGGGGGGATCCAGTACT | |
| Apta_489 | DNA | GGGGTTAAATATTCCCACATTGCCTGCGCCAGTACAAATAG | |
| Apta_586 | DNA | GGGAGACAAGGAAAATCCTTCAATGAAGTGGGTCGACA | |
| Apta_1269 | DNA | TAGCACACAACCCTTTGATTGGGATATCTCATCCCTCG | |
| Apta_835 | DNA | TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC |
DEV TOOL
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