Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1104
Aptamer sequence: CTTCTGCCCGCCTCCTTCCGGCGTCCCTGCGCACTGTATAACGTACACACGATACGTGGGAGACGAGATAGGCGGACACT
Target unique ID: P02144
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1028 | M01 | Protein | MYG_HUMAN | 98.0 nM | Ammonium bicarbonate buffer (50 mM, pH 8.5) containing 500 mM NaCl | 32631049 |
Structure information of aptamer
| Aptamer Sequence: | CTTCTGCCCGCCTCCTTCCGGCGTCCCTGCGCACTGTATAACGTACACACGATACGTGGGAGACGAGATAGGCGGACACT |
| The optimal secondary structure in dot-bracket notation: | ....((.((((((...((...((((((((((...(((((...))))).......)))))).)))).)).)))))).)).. |
| The centroid secondary structure in dot-bracket notation: | .....(.((((((...((...((((((((((....(((.....)))........)))))).)))).)).)))))).)... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1028 | Description | M01 |
| Aptamer chemistry | DNA | Length | 80 nt |
| GC content | 60.0% | Molecular weight | 24,537.67 Da |
| Molarity of 1 μg/μl solution | 40.75 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | CTTCTGCCCGCCTCCTTCCGGCGTCCCTGCGCACTGTATAACGTACACACGATACGTGGGAGACGAGATAGGCGGACACT | ||
| Applications | Detecting Mb in human serum and distinguishing acute myocardial infarction patients from healthy individuals | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P02144 | Protein name | Myoglobin |
| Gene name(s) | MB | Organism | Myoglobin |
| Status | reviewed in uniprot database | Length | 154 |
| Mass | 17,184 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | 3RGK; |
 |
Function | Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. |
Activity data
 |
Interaction ID | 1104 |
| Target type | Protein | |
| Target unique ID | P02144 | |
| Activity | 98.0 nM | |
| Binding Conditions/Buffer | Ammonium bicarbonate buffer (50 mM, pH 8.5) containing 500 mM NaCl |
|
| Assay | Characterization of selected Mb-binding aptamers |
|
| PubMed ID | 32631049 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1030 | DNA | CTTCTGCCCGCCTCCTTCCCGGGTTACAGGATCGTAAATCTTATTCGTTGCTCGATCGGGAGACGAGATAGGCGGACACT | |
| Apta_1074 | DNA | CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT | |
| Apta_1029 | DNA | CTTCTGCCCGCCTCCTTCCCGGTCGAGACCCGGGTTTACTTCCTCGGTAGTGTTCGAGGGAGACGAGATAGGCGGACACT | |
| Apta_1073 | DNA | CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1075 | DNA | CTTCTGCCCGCCTCCTTCCTACTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1076 | DNA | CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACACCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1212 | DNA | CTGTTCTCGGTCGCGACCTATCTGGTCGTCGCCGCGCTCGTGTTCGGAACGCTCATCGCGTTCGGGCTCGGCG | |
| Apta_657 | DNA | CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_741 | DNA | ATCGTCTGCTCCGTCCAATAGCCTCACCCTGCATAATTTATAGACTACACTTAGGAATCGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_508 | DNA | GCGGGCGATCGGCGGGGGGTGCGTGCGCTCTGTGCCAGGGGGTGGGACAGATCATATGGGGGTGCT | |
| Apta_527 | DNA | ATACGGGAGCCAACACCAACAACCTAGATGTTCTGATAACACGAATCGCTTCGAAGAGCAGGTGTGACGGAT | |
| Apta_740 | DNA | ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_867 | DNA | CTCCTCTGACTGTAACCACGCCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTGGCATAGGTAGTCCAGAAGCC | |
| Apta_908 | DNA | ATACGGGAGCCAACACCAATTCCGCCAAAGTCTGCTAATAAACGGACGCCGGGGAGAGCAGGTGTGACGGAT | |
| Apta_1048 | DNA | ATACGGGAGCCAACACCACCTGCGGAAAATCAACAACGACACACGCCACGATTCAGAGCAGGTGTGACGGAT | |
| Apta_246 | DNA | GGCTTTACACTTTATGCTTCCGGCCCGCGGAAATGATAACGCGTATGGTGGTATAACAATTTCACACAGGAAACAG | |
| Apta_450 | DNA | ATACCAGCTTATTCAATTACATGTCCTGAAGGGGAATAATATACAGCTTTGGGTGGGTAGATAGTAAGTGCAATCT | |
| Apta_451 | DNA | ATACCAGCTTATTCAATTGAGTAAGAGTGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCAATCT | |
| Apta_528 | DNA | ATACGGGAGCCAACACCACTTGGTTAACCAATCAAACCGGACAACGAAGGGTCCAGAGCAGGTGTGACGGAT | |
| Apta_529 | DNA | ATCCGTCACACCTGCTCTACACTCCGCACGATCTTTTTTAGAACGCGTACCCGTTGGTGTTGGCTCCCGTAT |
DEV TOOL
 |
 |
 |
 |