AptaDB: Result

Interaction ID: 1030

Aptamer sequence: CATGGCTAGGTGTTTATTAATCCTGTAGGATTTGCGGAAT

Target unique ID: P11166

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_947	A5	Protein	GTR1_HUMAN	160 nM	100 mg/mL of yeast tRNA (Thermo Fisher, AM7119) and 1 mg/mL of bovine serum albumin (RPI, A30075)	33184583

Aptamer Sequence:	CATGGCTAGGTGTTTATTAATCCTGTAGGATTTGCGGAAT
The optimal secondary structure in dot-bracket notation:	...........((((...(((((....)))))....))))
The centroid secondary structure in dot-bracket notation:	........................(((.....))).....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_947	Description	A5
Aptamer chemistry	DNA	Length	40 nt
GC content	40.0%	Molecular weight	12,386.97 Da
Molarity of 1 μg/μl solution	80.73 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	CATGGCTAGGTGTTTATTAATCCTGTAGGATTTGCGGAAT
Applications	Binding to other cell types with varying degrees of GLUT1 expression, and selective binding to vasculature in human brain cortical tissue

Type	Detail	Type	Detail
Uniprot ID	P11166	Protein name	Solute carrier family 2, facilitated glucose transporter member 1 (Glucose transporter type 1, erythrocyte/brain) (GLUT-1) (HepG2 glucose transporter)
Gene name(s)	SLC2A1 GLUT1	Organism	Solute carrier family 2, facilitated glucose transporter member 1 (Glucose transporter type 1, erythrocyte/brain) (GLUT-1) (HepG2 glucose transporter)
Status	reviewed in uniprot database	Length	492
Mass	54,084	Activity regulation	The uptake of glucose is inhibited by cytochalasin B and Phe-amide core-scaffold inhibitors GLUT-i1 and GLUT-i2
Involvement in disease	GLUT1 deficiency syndrome 1 (GLUT1DS1)	Catalytic activity	Reaction=D-glucose(out) = D-glucose(in)
PDB ID(s)	4PYP;5EQG;5EQH;5EQI;6THA;
Function	Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake.

	Interaction ID	1030
	Target type	Protein
	Target unique ID	P11166
	Activity	160 nM
	Binding Conditions/Buffer	100 mg/mL of yeast tRNA (Thermo Fisher, AM7119) and 1 mg/mL of bovine serum albumin (RPI, A30075)
	Assay	Aptamer A5 affinity analysis. Flow cytometry measurements of serially diluted (0-500 nM) FAM-labeled aptamer A5 binding to high-expressing GLUT1 or GLUT1-null Caco-2 cells. The FAM-labeled scrambled aptamer was used as a negative control with the high-expressing GLUT1 Caco-2 cells. Measurements were performed in duplicate with error bars representing mean 6 SD
	PubMed ID	33184583

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_184	DNA	AGGAAGGCTTTAGGTCTGAGATCTCGGAAT	62.50%
Apta_191	DNA	AGGAAGGCTTTACCTCTGAGATCTCGGAAT	62.50%
Apta_192	DNA	AGGAAGGCTTTAGCTCTGAGATCTCGGAAT	62.50%
Apta_193	DNA	AGGAAGGCTTTAGGGTCTGAGATCTCGGAAT	62.50%
Apta_195	DNA	AGGAAGGCTTTAGGGGTCTGAGATCTCGGAAT	62.50%
Apta_215	DNA	AGGAAGGCTTTAGGTCCGAGATCTCGGAAT	62.50%
Apta_186	DNA	AGGAACGCTTTAGGTCTGAGATCTCGGAAT	60.00%
Apta_194	DNA	AGGAAGGCTTTAGGGTCTGAGATCTCGGGAAT	60.00%
Apta_189	DNA	AGGAACCCTTTACCTCTGAGATCTCGGAAT	57.50%
Apta_190	DNA	AGGAACCCTTTAGGTCTGAGATCTCGGAAT	57.50%
Apta_202	DNA	AAGGCTTTAGGTCTGAGATCTCGGAAT	57.50%
Apta_597	DNA	TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG	55.10%
Apta_185	DNA	ACCAAGGCTTTAGGTCTGAGATCTCCCAAT	55.00%
Apta_197	DNA	GGCTTTAGGTCTGAGATCTCGGAAT	55.00%
Apta_206	DNA	AGGAAGGGGTCTGAGATCTCGGAAT	55.00%
Apta_420	DNA	AGGGACGGGCTAAGTAACTGTGGAGGGT	55.00%
Apta_564	DNA	GGGGACTGTTGTGGTATTATTTTTGGTTGTGCAGT	55.00%
Apta_783	DNA	AAGGGGTTGGGTGGGTTTATACAAATTAATTAATATTGTATGGTATATTT	54.00%
Apta_970	DNA	TTATGTAGCATTTATGAAATTTTTAAATCAATTTACTATTGGTGGTATCC	54.00%
Apta_973	DNA	TATGTAATGTTGACGTAATTTATCTCTGTGAGAATAAGCAGGTGGTATCC	54.00%

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