Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 867
Aptamer sequence: AAGGGGTTGGGTGGGTTTATACAAATTAATTAATATTGTATGGTATATTT
Target unique ID: TIB-202
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_783 | K19 | Cell | NB4 AML cell line | 12.37 nM | 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS) | 24405684 |
Structure information of aptamer
| Aptamer Sequence: | AAGGGGTTGGGTGGGTTTATACAAATTAATTAATATTGTATGGTATATTT |
| The optimal secondary structure in dot-bracket notation: | .........((((...(((((((((((....))).))))))))..)))). |
| The centroid secondary structure in dot-bracket notation: | .........((((...((((((((...........))))))))..)))). |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_783 | Description | K19 |
| Aptamer chemistry | DNA | Length | 50 nt |
| GC content | 28.0% | Molecular weight | 15,593.05 Da |
| Molarity of 1 μg/μl solution | 64.13 μM | Number of G-quadruplexes | 2 |
| G-Score | 19 | Function | Aptasensor |
| Sequence | AAGGGGTTGGGTGGGTTTATACAAATTAATTAATATTGTATGGTATATTT | ||
| Applications | Probes for acute myelogenous leukemia detection and surface protein biomarker discovery | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | TIB-202 | Cell name | NB4 AML cell line |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | monocyte |
| Morphology | monocyte | Tissue | Peripheral blood |
| Disease | Acute monocytic leukemia | Product format | Frozen |
| Antigen expression | HLA A2, A9, B5, DRw1, DRw2 | Genes expressed | Lysozyme; HLA: (A2; A9; B5; DRw2) |
| Cell description | THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research. |
|
|
| Application(s) | 3D cell culture; Immune system disorder research; Immunology; Toxicology | ||
Activity data
 |
Interaction ID | 867 |
| Target type | Cell | |
| Target unique ID | TIB-202 | |
| Activity | 12.37 nM | |
| Binding Conditions/Buffer | 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS) |
|
| Assay | Determination of the aptamer binding affinities to NB4 cells |
|
| PubMed ID | 24405684 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_607 | DNA | TGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTC | |
| Apta_608 | DNA | TGAGGCGGGTGGGTGGGTTGAATACGCTGATCACCCCATCGGAGAACGTTAAGGCGCTTT | |
| Apta_487 | DNA | CGGGGAGTTGGGAGGGGGGGCAAAGCCTGTGTATTTATCTTCTGT | |
| Apta_947 | DNA | CATGGCTAGGTGTTTATTAATCCTGTAGGATTTGCGGAAT | |
| Apta_611 | DNA | GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTTGCTTAGCTTCTACGGTGGGCTATCT | |
| Apta_889 | DNA | ACACTCTTTTGCTCGTGTTTTTGCCTGTTACATAAAATGAATCAGTGGATGTTTCCTTCT | |
| Apta_979 | DNA | ATGAGTAGTGCGGAGGGATGTGAATACTGACGCGGTCATAGTCGCTGTTGTACCATTGA | |
| Apta_179 | DNA | TCGGTTGAGTGAGATTAGTCAAATGTTTAGAAGACAACCTC | |
| Apta_746 | DNA | AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT | |
| Apta_609 | DNA | GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT | |
| Apta_610 | DNA | GCTGTGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT | |
| Apta_1230 | DNA | GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG | |
| Apta_988 | DNA | AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG | |
| Apta_884 | DNA | ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT | |
| Apta_35 | DNA | TGGGTGGCCTCCCGTTTATTCGAGATTTGGTTGAATTTGT | |
| Apta_50 | DNA | AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG | |
| Apta_173 | DNA | AGCAGCACAGAGGTCAGATGGCAGGTTTCGACGTACAATGCTATGGAGGCTTTATGATCGCCTATGCGTGCTACCGTGAA | |
| Apta_178 | DNA | AGCAGCACAGAGGTCAGATGCGGTATGCTGGGTCATCTAACTGGGGATATTCTGATATTGCCTATGCGTGCTACCGTGAA | |
| Apta_183 | DNA | TAGCACTGTGCGGCGCGTATTGTTCTAATCTGATTACATT | |
| Apta_216 | DNA | TGGTTGGTTTTTGGTTTTTTTTTTTGGTTT |
DEV TOOL
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