Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1029
Aptamer sequence: GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT
Target unique ID: Other-38
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_943 | QA-9 | Other | Soft-shelled turtle iridovirus (STIV) | 77.5 nM | 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris-HCl (pH 7.5) | 26419355 |
Structure information of aptamer
| Aptamer Sequence: | GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT |
| The optimal secondary structure in dot-bracket notation: | ...........((((((....((((((..........)))))))))))). |
| The centroid secondary structure in dot-bracket notation: | ...........((((((....((((((..........)))))))))))). |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_943 | Description | QA-9 |
| Aptamer chemistry | DNA | Length | 50 nt |
| GC content | 48.0% | Molecular weight | 15,603.04 Da |
| Molarity of 1 μg/μl solution | 64.09 μM | Number of G-quadruplexes | 2 |
| G-Score | 19 | Function | Targeted therapy |
| Sequence | GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT | ||
| Applications | Inhibited STIV infection in vitro and in vivo, with aptamer QA-36 demonstrating the greatest protective effect against STIV and inhibiting STIV infection in a dose-dependent manne | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-38 | Name | Soft-shelled turtle iridovirus (STIV) |
Activity data
 |
Interaction ID | 1029 |
| Target type | Other | |
| Target unique ID | Other-38 | |
| Activity | 77.5 nM | |
| Binding Conditions/Buffer | 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris-HCl (pH 7.5) |
|
| Assay | Affinity of STIV-selected ssDNA aptamer interactions by ELISA. STIV was incubated with increasing concentrations of 5′-biotinylated aptamer. After addition of streptavidin-HRP, the amount of STIV-aptamer complex was calculated and graphed as a function of aptamer concentration. The graph was fit to the equation Y = Bmax*X/(Kd + X) using SigmaPlot software. Results for each aptamer was presented as the mean ± SD of three independent experiments |
|
| PubMed ID | 26419355 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1260 | DNA | GAAAGGGACGTTCCAAGTTCGTATAAGCAGTCCTGTGCGT | |
| Apta_258 | DNA | CACTGCGGGGGTCTATACGTGAGGAAGAAGTGGGCAGGTC | |
| Apta_1093 | DNA | AGGAGGGTAGGTAGTGCTTGGTAGGGAAACTCCGCCGATT | |
| Apta_1138 | DNA | GCAGTGTGGGCGAATCTATGCGTACCGTTCGATATCGTG | |
| Apta_1110 | DNA | GTTGACGGATCCCTATTGCAGTGCTTGAGAGAGACCTCGCGTATATTTCGGAAGACCACGTGTAATGCCCCTAACT | |
| Apta_608 | DNA | TGAGGCGGGTGGGTGGGTTGAATACGCTGATCACCCCATCGGAGAACGTTAAGGCGCTTT | |
| Apta_159 | DNA | GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT | |
| Apta_160 | DNA | CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA | |
| Apta_485 | DNA | TGTCGTTGGGGGGGTAGGGAAGGGTGAAACGACAAGCCAGAAGTT | |
| Apta_491 | DNA | TTGGGGTCTGCTCGGGATTGCGGAGAACGTGAATCT | |
| Apta_585 | DNA | GATCGAGGGCAGCGATAGCTGGGCTAATAAGGTTAGCCCCATCGGTC | |
| Apta_640 | DNA | CAATGGACCTATTCGAGTACTGAATAGAACAGTCGGCGCTCTGGG | |
| Apta_778 | DNA | GAAGACGAGCGGCGAGTGTTATTACGCTTGGAAACAACCCC | |
| Apta_778 | DNA | GAAGACGAGCGGCGAGTGTTATTACGCTTGGAAACAACCCC | |
| Apta_857 | DNA | GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC | |
| Apta_1254 | DNA | GGCAGTGTGGGCGAATCTATGCGTACCGTTCGATATCGTG | |
| Apta_1258 | DNA | GGCGAAGGAGTCATGTAGGTGTGGTCGAGACCGCTGTGCT | |
| Apta_1261 | DNA | GGGCGGACGCTAGGTGGTGATGCTGTGCTACACGTGTTGT | |
| Apta_1262 | DNA | ACAAGGGGTGCAGGGGTCCGCGATTGTGACTGTGTGTGCG | |
| Apta_1213 | DNA | GGACTTGAAGAAAAGGGCGACACGCGAAGTCGATGTCGCGTCTGCCTGAAGTCA |
DEV TOOL
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