Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1074
Aptamer sequence: ATGCGGGTACATCTCTCCAACCAGTCAAACTGCTATGATAAGACCTCATTCAACCCCGGTA
Target unique ID: P35270
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_989 SP5 Protein SPRE_HUMAN 47.0 nM 50 mM Tris, pH 7.5, 150 mM NaCl and 2 mM MgCl2 32456943
Structure information of aptamer
Aptamer Sequence: ATGCGGGTACATCTCTCCAACCAGTCAAACTGCTATGATAAGACCTCATTCAACCCCGGTA
The optimal secondary structure in dot-bracket notation: ...((((..............((((...))))..((((.......)))).....))))...
The centroid secondary structure in dot-bracket notation: .....................((((...)))).............................
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_989 Description SP5
Aptamer chemistry DNA Length 61 nt
GC content 47.5% Molecular weight 18,620.98 Da
Molarity of 1 μg/μl solution 53.70 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Aptasensor
Sequence ATGCGGGTACATCTCTCCAACCAGTCAAACTGCTATGATAAGACCTCATTCAACCCCGGTA
Applications Fabricate a competitive aptasensor for sepiapterin detection
Target information
Type Detail Type Detail
Uniprot ID P35270 Protein name Sepiapterin reductase (SPR) (EC 1.1.1.153)
Gene name(s) SPR Organism Sepiapterin reductase (SPR) (EC 1.1.1.153)
Status reviewed in uniprot database Length 261
Mass 28,048 Activity regulation N/A
Involvement in disease Dystonia, DOPA-responsive, due to sepiapterin reductase deficiency (DRDSPRD) Catalytic activity Reaction=L-erythro-7,8-dihydrobiopterin + NADP(+) = H(+) + NADPH + sepiapterin
PDB ID(s)
1Z6Z;4HWK;4J7U;4J7X;4XWY;4Z3K;6I6C;6I6F;6I6P;6I6T;6I6V;6I79;6USN;7DSF;
Function Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
Activity data
Interaction ID 1074
Target type Protein
Target unique ID P35270
Activity 47.0 nM
Binding Conditions/Buffer

50 mM Tris, pH 7.5, 150 mM NaCl and 2 mM MgCl2
Assay

The fluorescence binding affinity curves for six sepiapterin aptamers (a plot of the aptamer concentration versus the fluorescence intensity of the eluted aptamer solution). The dissociation constants were calculated via nonlinear regression analysis of the saturation curves and the error bars represent the standard deviation of the measurements
PubMed ID 32456943
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_511 DNA ATCCGTCACACCTGCTCTCAGGGGATCACATTCTTGACGGTGTGATACAGTGCCTGGTGTTGGCTCCCGTAT 55.56%
Apta_536 DNA ATCCGTCACACCTGCTCTCGAATAGAGTACTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT 54.17%
Apta_899 DNA ATCCGTCACACCTGCTCTCGAATAGAGTATTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT 54.17%
Apta_395 DNA AATCCACGTATCGACTAGCTCAAACCAGCCTGATAGAGCCATTCGCTTTA 54.10%
Apta_512 DNA ATAGGACGATTATCGAAAATCACCAGATTGGACCCTGGTTAACGAT 54.10%
Apta_983 DNA ATGGGAACAGAGCGGCGCAGAGACCCAATGCCACGAGTACCTGACCCTATCAACCCGTGA 54.10%
Apta_1162 DNA AGAGGCTACAGCGATAAGTCGACATTGCTGACCGTACCTAGTAATACGT 54.10%
Apta_532 DNA ATCCGTCACACCTGCTCTAACTTACACATAATTCTAGGTTACATCTTGCTCATATGGTGTTGGCTCCCGTAT 52.78%
Apta_537 DNA ATCCGTCACACCTGCTCTCCTCGAACTAGTTAGACCCTCCTCCAAGTCAACTTGTGGTGTTGGCTCCCGTAT 52.78%
Apta_826 DNA ATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGGATGGTGTTGGCTCCCGTAT 52.78%
Apta_1051 DNA ATCCGTCACACCTGCTCTATACACATTGTGTTTACTCCCAGTTTTTTAGACTTATGGTGTTGGCTCCCGTAT 52.78%
Apta_921 DNA ATCCAGAGTGACGCAGCACGAGCCAGACATCTCACACCTGTTGCATATACATTTTGCATGGACACGGTGGCTTAGT 52.63%
Apta_108 DNA AGCAGCACAGAGGTCAGATGCTTTGCCACGGCAACGTCCCAAAACTAATGGTAACTATACCCTATGCGTGCTACCGTGAA 52.50%
Apta_108 DNA AGCAGCACAGAGGTCAGATGCTTTGCCACGGCAACGTCCCAAAACTAATGGTAACTATACCCTATGCGTGCTACCGTGAA 52.50%
Apta_177 DNA AGCAGCACAGAGGTCAGATGCCATAGGTGCCTGAATTGTCCCAAACATTCGCATGATCGACCTATGCGTGCTACCGTGAA 52.50%
Apta_881 DNA AGCGTCGAATACCACTACAGCCCGCACTCAACCACCGTTCCTTTGTTTAATTTTGCACATCTAATGGAGCTCGTGGTCAG 52.50%
Apta_335 DNA ACCGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTG 52.46%
Apta_635 DNA TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCA 52.46%
Apta_636 DNA TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA 52.46%
Apta_638 DNA TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCG 52.46%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

HELP CENTER

>Contact us
>LMMD Group
>School of Pharmacy

DEV TOOL
Page last updated at 2022-05-25 10:01:57 (Asia/Shanghai) | You are visitor No. 43983
Copyright © 2019-2020 Laboratory of Molecular Modeling and Design, School of Pharmacy, East China University of Science and Technology. All rights reserved.