Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 50
Aptamer sequence: AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG
Target unique ID: P00432
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_50 | CAT 4 | Protein | CATA_BOVIN | 2.49 μM | 25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3) | 22965726 |
Structure information of aptamer
| Aptamer Sequence: | AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG |
| The optimal secondary structure in dot-bracket notation: | ...((((..(((((((.......)))))))....)))). |
| The centroid secondary structure in dot-bracket notation: | ....(((..(((((((.......)))))))....))).. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_50 | Description | CAT 4 |
| Aptamer chemistry | DNA | Length | 39 nt |
| GC content | 51.3% | Molecular weight | 12,253.86 Da |
| Molarity of 1 μg/μl solution | 81.61 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG | ||
| Applications | Aptamer exhibited a distinct specificity toward bovine catalase | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P00432 | Protein name | Catalase (EC 1.11.1.6) |
| Gene name(s) | CAT | Organism | Catalase (EC 1.11.1.6) |
| Status | reviewed in uniprot database | Length | 527 |
| Mass | 59,915 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | Reaction=2 H2O2 = 2 H2O + O2 |
| PDB ID(s) | 1TGU;1TH2;1TH3;1TH4;3J7B;3NWL;3RE8;3RGP;3RGS;4BLC;5GKN;6JNT;6JNU;6PM7;6PO0;7CAT;7DI8;8CAT; |
 |
Function | Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. |
Activity data
 |
Interaction ID | 50 |
| Target type | Protein | |
| Target unique ID | P00432 | |
| Activity | 2.49 μM | |
| Binding Conditions/Buffer | 25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3) |
|
| Assay | N/A |
|
| PubMed ID | 22965726 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_756 | DNA | GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT | |
| Apta_124 | DNA | GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC | |
| Apta_13 | DNA | AGGGGGGGGGGAGGGGTCGGGGTGG | |
| Apta_28 | DNA | AGTGGGGGGGGTGGAGCCGGGTAGA | |
| Apta_674 | DNA | GCACGATGGGGAAAGGGTCCCCCTGGGTTG | |
| Apta_1210 | DNA | GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG | |
| Apta_1237 | DNA | GGGGTTGTGTGGGGAGTTTGTCCTGTTGTG | |
| Apta_1239 | DNA | GGTGGATGGGGATGCTTTGTTAGTTTGTGC | |
| Apta_1241 | DNA | GGTGTGGAGTGGTGTGTGTTGTTGTTTGTC | |
| Apta_1245 | DNA | AGTGTGAAGTGGTGTGTGTTGTTGTTTGTC | |
| Apta_484 | DNA | CAGGGAGGGACAGGGCGGGAATTTGTATGATGGTTCGTTTGCTCG | |
| Apta_749 | DNA | GATCGGTGGGTGGGGGGGTTGGAGATCATCCTCAGGGATTACGTC | |
| Apta_143 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAGTTTCG | |
| Apta_691 | DNA | CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT | |
| Apta_693 | DNA | CGAGTTGAGCCGGGCGCGGTACGGGTACTGGTATGTGTGG | |
| Apta_1230 | DNA | GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG | |
| Apta_1261 | DNA | GGGCGGACGCTAGGTGGTGATGCTGTGCTACACGTGTTGT | |
| Apta_144 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG | |
| Apta_991 | DNA | AGCAGTGAAGAGGCGAGAGATTTATGAGGGTAGCGTCCACATATGCCAGGATCGTGTTG | |
| Apta_14 | DNA | AGCGGGGGGGGCTGGGCTGGGTGGG |
DEV TOOL
 |
 |
 |
 |