Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1241
Aptamer sequence: GAATTCAGTCGGACAGCGAAGTAGTTTTCCTTCTAACCTAAGAACCCGCGGCAGTTTAATGTAGATGGACGAATACGTCTCCC
Target unique ID: CRM-HTB-26
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1179 LXL-1 Cell MDA-MB-231 cells 44 nM 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL BSA in PBS, pH7.4 24892693
Structure information of aptamer
Aptamer Sequence: GAATTCAGTCGGACAGCGAAGTAGTTTTCCTTCTAACCTAAGAACCCGCGGCAGTTTAATGTAGATGGACGAATACGTCTCCC
The optimal secondary structure in dot-bracket notation: ..........(((.(((......))).)))((((......))))......(((......)))....(((((....)))))...
The centroid secondary structure in dot-bracket notation: ..........(((.(((......))).)))......................................((......)).....
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1179 Description LXL-1
Aptamer chemistry DNA Length 83 nt
GC content 47.0% Molecular weight 25,568.40 Da
Molarity of 1 μg/μl solution 39.11 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Target detection
Sequence GAATTCAGTCGGACAGCGAAGTAGTTTTCCTTCTAACCTAAGAACCCGCGGCAGTTTAATGTAGATGGACGAATACGTCTCCC
Applications Recognition and tissue imaging
Target information
Type Detail Type Detail
ATCC ID CRM-HTB-26 Cell name MDA-MB-231 cells
Category Human cells Organism Homo sapiens, human
Product type Certified reference material Cell type epithelial cell
Morphology epithelial Tissue Breast; Mammary gland
Disease Adenocarcinoma Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description N/A
Application(s) Assay development
Activity data
Interaction ID 1241
Target type Cell
Target unique ID CRM-HTB-26
Activity 44 nM
Binding Conditions/Buffer

4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL BSA in PBS, pH7.4
Assay

Dissociation constant of selected aptamers for MDA-MB-231
PubMed ID 24892693
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_774 DNA GAATTCAGTCGGACAGCGAGGAAAATTTCGTTATTTTCGTTTCCGGGAACTCGGGCATTTAGAGATGGACGAATATCGTCTCCC 72.62%
Apta_770 DNA GAATTCAGTCGGACAGCGGCATTTACATTCCATGGGAAATTTCTCAAGCGGTATTTCATTTCGATGGACGAATATCGTCTCCC 71.08%
Apta_1182 DNA GAATTCAGTCGGACAGCGTCAGTCGTCCCTTTATGCTTGGTGCTCACCGTCTGAATTTCTTCAGATGGACGAATATCGTCTCCC 70.24%
Apta_1181 DNA GAATTCAGTCGGACAGCGTAGTAAGAATCTGTTTCTGGTCCTAGTCTTGCTTGTGTTTCCTGATGGACGAATATCGTCTCCC 69.88%
Apta_1180 DNA GAATTCAGTCGGACAGCGCAATTGTGGTTCTTACCCTATCCCTTGTGTTTGGCGTTCGTTGCGATGGACGAATATCGTCTCCC 68.67%
Apta_767 DNA GAATTCAGTCGGACAGCGACGGTGGGAGCCCCAAATAATTCTTGCGATTATTAGTGTAAGCGGATGGACGAATATCGTCTCCC 67.47%
Apta_1184 DNA GAATTCAGTCGGACAGCGTAATCCCTTGTGTTTGACGTTCGAAGATTATTTCTATCTTTTAGGATGGACGAATATCGTCTCCC 66.27%
Apta_768 DNA GAATTCAGTCGGACAGCGGCACTTGCGACACGTTTGTCGGGTAAATGCGTGTATTTTCTTTTCGATGGACGAATATCGTCTCCC 65.48%
Apta_1183 DNA GAATTCAGTCGGACAGCGGCGTGAGTCTTTTGCGGTGGCTCGGGTTCGAATATCTACGACCTTGATGGACGAATATCGTCTCCC 64.29%
Apta_772 DNA GAATTCAGTCGGACAGCGCGGTGTTGGTGGTGGGGTGGTTTGGGAATCTGTTCGGCTGGAGATGGACGAATATCGTCTCCC 63.86%
Apta_769 DNA GAATTCAGTCGGACAGCGAGTTGTTGTTAGGGTGTTGGGTTGGGGTTTTAGGGTCCCTGTCGTCGATGGACGAATATCGTCTCCC 63.53%
Apta_775 DNA GAATTCAGTCGGACAGCGCGGTGTTGGTGGCGGGGGTGGTTGGGGGATTTGGCTGTCCTGAGTGATGGACGAATATCGTCTCCC 63.10%
Apta_773 DNA GAATTCAGTCGGACAGCGGTGGTGCTTGTGTATGGGGGTGGTTGGTGGGTTTTATGCTGCTGGATGGACGAATATCGTCTCCC 62.65%
Apta_771 DNA GAATTCCGTCGGACAGCGGTATGCATGGGAGGTTCTGGAGGGGGTGGGTTGGCGTGCGTTCCAGATGGACGAATATCGTCTCCC 59.52%
Apta_573 DNA CGTACAGTGGGCTATATTGCTGACGTAAGAGCTGCTTTCAATGCATTGGCATAGATGTCA 53.01%
Apta_550 DNA GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATTTTACAGAACAACCAACGTCGCTCCGGGTACTTCTTCATCGAGATAGTAAGTGCAATCT 53.00%
Apta_933 DNA GACGCTTACTCAGGTGTGACTCGGGGTGTAGCTCGTTATGATTCGGACAAGACTTACCTTGCGCCTCTGGGATCGAAGGACGCAGATGAAGTCTC 52.63%
Apta_128 DNA GGTATTGAGGGTCGCATCCCGTAGTGTGTTCTTATTCGTGTCTGTGTGTGTTCTGTCGGATGGCTCTAACTCTCCTCT 51.81%
Apta_1111 DNA GTGCGTCGCATGATCGCGATTAGTGCGACCATGTACGATTCTCACGACAACGACACTGTACTTGAGGACTGTCTCC 51.81%
Apta_1129 DNA GCCGAGATTGCACTTACTATCTGATGTTATTGTGTTTATTGGAATTTAAAGTATATGGGATGTTTGGTTAGTAATTGAATAAGCTGGTATGCGC 51.06%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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