AptaDB: Result

Interaction ID: 484

Aptamer sequence: CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT

Target unique ID: Q61727

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_479	R5D1	Protein	I10R1_MOUSE	230 nM	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature	22434135

Aptamer Sequence:	CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT
The optimal secondary structure in dot-bracket notation:	(((((......)))))((((((...)))))).........
The centroid secondary structure in dot-bracket notation:	.((((......)))).((((((...)))))).........

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_479	Description	R5D1
Aptamer chemistry	DNA	Length	40 nt
GC content	57.5%	Molecular weight	12,307.86 Da
Molarity of 1 μg/μl solution	81.25 μM	Number of G-quadruplexes	2
G-Score	17	Function	Targeted therapy
Sequence	CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT
Applications	Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro

Type	Detail	Type	Detail
Uniprot ID	Q61727	Protein name	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Gene name(s)	Il10ra Il10r	Organism	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Status	reviewed in uniprot database	Length	575
Mass	64,248	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation.

No relevant experimental diagram	Interaction ID	484
	Target type	Protein
	Target unique ID	Q61727
	Activity	230 nM
	Binding Conditions/Buffer	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature
	Assay	N/A
	PubMed ID	22434135

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_480	DNA	CGGCACACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT	97.50%
Apta_481	DNA	CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTA	97.50%
Apta_914	DNA	CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT	57.50%
Apta_1257	DNA	CGACGCACAGTCGCTGGTGCGTACCTGGTTGCCGTTGTGT	57.50%
Apta_1261	DNA	GGGCGGACGCTAGGTGGTGATGCTGTGCTACACGTGTTGT	57.50%
Apta_63	DNA	GACCCGTGGTAGGGTAGGATGGGGTGGTC	55.00%
Apta_74	DNA	GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC	55.00%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	55.00%
Apta_692	DNA	ACGTTGACGCTGGTGCCCGCTTGTGGTGCGAGTGTTGTGT	55.00%
Apta_858	DNA	GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG	55.00%
Apta_986	DNA	CGTGTATCCCCTGTGTGTTTGTACTCGGCTACTGTATCCG	55.00%
Apta_1205	DNA	GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG	55.00%
Apta_1255	DNA	GGCGCATGATCATTCGGTGGGTAAGGTGGTGGTAACGTTG	55.00%
Apta_750	DNA	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT	53.33%
Apta_143	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAGTTTCG	53.06%
Apta_35	DNA	TGGGTGGCCTCCCGTTTATTCGAGATTTGGTTGAATTTGT	52.50%
Apta_69	DNA	AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT	52.50%
Apta_71	DNA	GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC	52.50%
Apta_72	DNA	AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT	52.50%
Apta_482	DNA	AGCAAGGTTTGGTGTTGGTTGGTTGCTGGTTTTGGTTTC	52.50%

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