AptaDB: Result

Interaction ID: 1239

Aptamer sequence: TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA

Target unique ID: CRL-1690

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1177	WYZ-50a	Cell	T98G [T98-G]	2.8 nM	4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL BSA in Dulbecco’s PBS	29708252

Aptamer Sequence:	TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA
The optimal secondary structure in dot-bracket notation:	((((((..((((...(((............)))...))))))))))
The centroid secondary structure in dot-bracket notation:	((((((...(............................).))))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1177	Description	WYZ-50a
Aptamer chemistry	DNA	Length	46 nt
GC content	43.5%	Molecular weight	14,174.08 Da
Molarity of 1 μg/μl solution	70.55 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA
Applications	Diagnosis and effective therapy of glioma

Type	Detail	Type	Detail
ATCC ID	CRL-1690	Cell name	T98G [T98-G]
Category	Human cells	Organism	Homo sapiens, human
Product type	N/A	Cell type	N/A
Morphology	fibroblast	Tissue	Brain
Disease	Glioblastoma Multiforme	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	3D cell culture; Neuroscience

	Interaction ID	1239
	Target type	Cell
	Target unique ID	CRL-1690
	Activity	2.8 nM
	Binding Conditions/Buffer	4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL BSA in Dulbecco’s PBS
	Assay	Kd of full-length and truncated aptamers for T98G cells
	PubMed ID	29708252

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1176	DNA	AGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACT	73.91%
Apta_682	DNA	CCTTGTAGATCGGGTGTGGTTTGGCGTAGG	58.70%
Apta_853	DNA	TGACCGAGTGTCTGGTCATTTTCGATGTCTGTTGTTAGGC	58.70%
Apta_1175	DNA	ATGAGAGCGTCGGTGTGGTATCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGAGGGTGCGGAAGTA	58.23%
Apta_1280	DNA	TCATATCCTTCTTGGTATCATTGCGTCATTTAGTCATTCATGTTATTCCCGAGGG	58.18%
Apta_58	DNA	GCACCTTGATGACATGATAGTCGTTGTGTATGCAGTTGGC	56.52%
Apta_681	DNA	CCTGACGATCGGGTGTGGGTTGGCTTGAGG	56.52%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	56.52%
Apta_1042	DNA	ATCACGATATGCCCGGCTCATCGGGGTTCAGTTGGGCGGTCACATGGAAA	56.00%
Apta_1273	DNA	TCCCTCCTGTTGGTTTGGGATGGTGGCGACTATCTGATTGTTTGACTCCCCGTAGGGG	55.17%
Apta_910	DNA	TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA	54.55%
Apta_476	DNA	CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG	54.35%
Apta_671	DNA	GCATCTGATCGGGTGTGGGTGGCGTAAAGG	54.35%
Apta_675	DNA	ACTGTCCGTCGGGTTTAGGGTGGCGTTCGG	54.35%
Apta_820	DNA	TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA	54.35%
Apta_858	DNA	GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG	54.35%
Apta_914	DNA	CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT	54.35%
Apta_1064	DNA	CGATGGAGGACCTCCGGTTTACCGTGTCGTTTTACTCTTG	54.35%
Apta_1066	DNA	CCTACGTTGGAGTGGGGTTTGCGCAGGCCGTTCTTTCCAA	54.35%
Apta_1216	DNA	AGCGTCCTCTGCATAGTGATTTACGCTATGTGTACAGTGC	54.35%

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