AptaDB: Result

Interaction ID: 1261

Aptamer sequence: CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG

Target unique ID: 27294

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1199	Aptamer 1	Cell	Mycobacterium tuberculosis subsp. Tuberculosis	37 nM	20 mmol/L HEPES buffer at pH 7.35, 1 mmol/L MgCl2, 120 mmol/L NaCl, 1 mmol/L CaCl2, and 5 mmol/L KCl	28689112

Aptamer Sequence:	CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG
The optimal secondary structure in dot-bracket notation:	.....((((....))))..................
The centroid secondary structure in dot-bracket notation:	...................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1199	Description	Aptamer 1
Aptamer chemistry	DNA	Length	35 nt
GC content	65.7%	Molecular weight	10,795.90 Da
Molarity of 1 μg/μl solution	92.63 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target detection
Sequence	CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG
Applications	Detection of H37Rv in clinical diagnosis

Type	Detail	Type	Detail
ATCC ID	27294	Cell name	Mycobacterium tuberculosis subsp. Tuberculosis
Category	Bacteria	Organism	N/A
Product type	N/A	Cell type	N/A
Morphology	N/A	Tissue	N/A
Disease	N/A	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	This strain has been assigned to Risk Group 3-agents associated with serious or lethal human disease for which preventive or therapeutic interventions may be available.
Application(s)	N/A

	Interaction ID	1261
	Target type	Cell
	Target unique ID	27294
	Activity	37 nM
	Binding Conditions/Buffer	20 mmol/L HEPES buffer at pH 7.35, 1 mmol/L MgCl2, 120 mmol/L NaCl, 1 mmol/L CaCl2, and 5 mmol/L KCl
	Assay	Binding saturation curve of Aptamer 1 to the target H37Rv
	PubMed ID	28689112
No relevant experimental diagram	Interaction ID	1263
	Target type	Cell
	Target unique ID	27294
	Activity	101 nM
	Binding Conditions/Buffer	20 mmol/L HEPES buffer at pH 7.35, 1 mmol/L MgCl2, 120 mmol/L NaCl, 1 mmol/L CaCl2, and 5 mmol/L KCl
	Assay	Binding saturation curve of Aptamer 1 to the target H37Rv
	PubMed ID	28689112
No relevant experimental diagram	Interaction ID	1264
	Target type	Cell
	Target unique ID	27294
	Activity	98 nM
	Binding Conditions/Buffer	20 mmol/L HEPES buffer at pH 7.35, 1 mmol/L MgCl2, 120 mmol/L NaCl, 1 mmol/L CaCl2, and 5 mmol/L KCl
	Assay	Binding saturation curve of Aptamer 1 to the target H37Rv
	PubMed ID	28689112
No relevant experimental diagram	Interaction ID	1269
	Target type	Cell
	Target unique ID	27294
	Activity	108 nM
	Binding Conditions/Buffer	20 mmol/L HEPES buffer at pH 7.35, 1 mmol/L MgCl2, 120 mmol/L NaCl, 1 mmol/L CaCl2, and 5 mmol/L KCl
	Assay	Binding saturation curve of Aptamer 1 to the target H37Rv
	PubMed ID	28689112

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1199	DNA	CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG	100.00%
Apta_1199	DNA	CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG	100.00%
Apta_1199	DNA	CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG	100.00%
Apta_1208	DNA	CCCTGCGGGGCTGCCCGATATGTGTCCAGGTGGTG	97.14%
Apta_1203	DNA	CCCTGCGGGGCTACCCGATATGTGTCCGAGTGGTG	94.29%
Apta_692	DNA	ACGTTGACGCTGGTGCCCGCTTGTGGTGCGAGTGTTGTGT	62.50%
Apta_57	DNA	GCGGGTCGGTTGCTCGCTTCGCCCGATCGGTCTAAGGGTG	60.00%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	57.50%
Apta_1040	DNA	CAATTGGGCCCGTCCGTATGGTGGGT	57.14%
Apta_144	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG	56.82%
Apta_340	DNA	TACTCAGGGCACTGCAAGCAATTGTGGTCCCAATGGGCTGAGTA	56.82%
Apta_829	DNA	CCCTCCGGGGGGGTCATCGGGATACCTGGTAAGGATA	56.76%
Apta_1059	DNA	TCGTGACGCCGTGCAGTTACCGATACGAGCCTCGTTTTG	56.41%
Apta_552	DNA	CACCTGGGGGAGTATTGCGGAGGAAGGTTCCAGGTG	55.56%
Apta_722	DNA	CTCTCGGGACGACGGCCCGATCTCAGAGTAGTCGTCCC	55.26%
Apta_148	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG	55.00%
Apta_149	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGG	55.00%
Apta_1262	DNA	ACAAGGGGTGCAGGGGTCCGCGATTGTGACTGTGTGTGCG	55.00%
Apta_146	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG	54.29%
Apta_250	DNA	CCCTGGCCGCCCTCTGGCGCGCTGATGT	54.29%

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