Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1237
Aptamer sequence: ATGAGAGCGTCGGTGTGGTATCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGAGGGTGCGGAAGTA
Target unique ID: CRL-1690
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1175 WYZ-50 Cell T98G [T98-G] 16.1 nM 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL BSA in Dulbecco’s PBS 29708252
Structure information of aptamer
Aptamer Sequence: ATGAGAGCGTCGGTGTGGTATCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGAGGGTGCGGAAGTA
The optimal secondary structure in dot-bracket notation: .................(((((((.(((((((...(((............)))...))))).)).))))))).......
The centroid secondary structure in dot-bracket notation: .................((((((..(((((((...(((............)))...))))).))..)))))).......
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1175 Description WYZ-50
Aptamer chemistry DNA Length 79 nt
GC content 49.4% Molecular weight 24,630.72 Da
Molarity of 1 μg/μl solution 40.60 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted therapy
Sequence ATGAGAGCGTCGGTGTGGTATCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGAGGGTGCGGAAGTA
Applications Diagnosis and effective therapy of glioma
Target information
Type Detail Type Detail
ATCC ID CRL-1690 Cell name T98G [T98-G]
Category Human cells Organism Homo sapiens, human
Product type N/A Cell type N/A
Morphology fibroblast Tissue Brain
Disease Glioblastoma Multiforme Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description N/A
Application(s) 3D cell culture; Neuroscience
Activity data
Interaction ID 1237
Target type Cell
Target unique ID CRL-1690
Activity 16.1 nM
Binding Conditions/Buffer

4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL BSA in Dulbecco’s PBS
Assay

Kd of full-length and truncated aptamers for T98G cells
PubMed ID 29708252
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_1174 DNA ATGAGAGCGTCGGTGTGGTAAGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACTCTTGCTGTAGGAGGGTGCGGAAGTA
80.72%
Apta_803 DNA ATGAGAAGCGTCGGTGTGGTTACTCCGGGCACTTGATATATCGATATGGAGAATAATGCACCCTGAGCGGGCTGGCAAGGCGCATA 60.47%
Apta_802 DNA ATGAGAAGCGTCGGTGTGGTTAAACACGGAACGAAGGTGCAGGAAGATTTGTCGATGCGGTGCCTGAGCGGGCTGGCAAGGCGCATA 58.62%
Apta_1177 DNA TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA 58.23%
Apta_732 DNA AAGGAGCAGCGTGGAGGATAGCTGTCATCGGCTTATGTGCTTTCCAGGGTCCGTGCTGTCCGTTGTTAGGGTGTGTCGTCGTGGT 56.47%
Apta_786 DNA ATCCAGAGTGACGCAGCATTTGTATCCTATTTTGTTTATGTAATTGTCGTTGATCATGTGGACACGGTGGCTTAGT 55.70%
Apta_923 DNA ATCCAGAGTGACGCAGCATCGACCTCTGTGCGAGCGGGTTGCATATATATTTTGCATGGACACGGTGGCTTAGT 55.70%
Apta_725 DNA ATCCAGAGTGACGCAGCATGCTCATCCACGATTCTGGCGAATTTAGTGCCTGTCTTTTTCTCTTGGACACGGTGGCTTAGT 55.56%
Apta_800 DNA AAGGAGCAGCGTGGAGGATATACACCGTCATCAGAGGGAGCATCTCTAGTCAGGACTGTGATGAATTAGGGTGTGTCGTCGTGGT 55.29%
Apta_616 DNA GGTGCGGGTAGGGATGAGGGCGGGTCGGGTTGAGTGTGAGCTTAGGGGtGGCGGATCTAC 54.43%
Apta_653 DNA CCTGCACCTCGTCCAGCATCCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGGTG 54.43%
Apta_655 DNA CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTG 54.43%
Apta_731 DNA AAGGAGCAGCGTGGAGGATACTCTAATTGCCGTATAAGGTCAGGGGGTTGGTTGGTTCCTAGTGCTTAGGGTGTGTCGTCGTGGT 54.12%
Apta_531 DNA ATACGGGAGCCAACACCACATCGACTTACGAATCAACGCGTTTATTATTGGTTCAGAGCAGGTGTGACGGAT 53.16%
Apta_656 DNA GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC 53.16%
Apta_734 DNA TCCTCGATGCACCAGTTCACCTTATTTGCTTCTTCTCTCTGTTCTGACTGGGTGCTGAGGA 53.16%
Apta_784 DNA ATCCAGAGTGACGCAGCATTTTGACGCTTTATCCTTTTCTTATGGCGGGATAGTTTCGTGGACACGGTGGCTTAGT 53.16%
Apta_785 DNA ATCCAGAGTGACGCAGCATGAATCTTTTCTTTTGGTTTTGATATTTATAGTTGGTGAATGGACACGGTGGCTTAGT 53.16%
Apta_801 DNA TGCGTGTGTAGTGTGTCTGCATGCCCCTGTAATCGCCCATGGGTAGCCTCTTAGGGATTTGGGCGG 53.16%
Apta_922 DNA ATCCAGAGTGACGCAGCACACCGTTTGTATTCTGCATTGTTTTGCATTCTACATGGACACGGTGGCTTAGT 53.16%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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