Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 486
Aptamer sequence: CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTA
Target unique ID: Q61727
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_481 | R5D3 | Protein | I10R1_MOUSE | N/A | 150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature | 22434135 |
Structure information of aptamer
| Aptamer Sequence: | CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTA |
| The optimal secondary structure in dot-bracket notation: | (((((......)))))((((((...))))))......... |
| The centroid secondary structure in dot-bracket notation: | .((((......)))).((((((...))))))......... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_481 | Description | R5D3 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 57.5% | Molecular weight | 12,316.87 Da |
| Molarity of 1 μg/μl solution | 81.19 μM | Number of G-quadruplexes | 2 |
| G-Score | 17 | Function | Targeted therapy |
| Sequence | CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTA | ||
| Applications | Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | Q61727 | Protein name | Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210) |
| Gene name(s) | Il10ra Il10r | Organism | Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210) |
| Status | reviewed in uniprot database | Length | 575 |
| Mass | 64,248 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 486 |
| Target type | Protein | |
| Target unique ID | Q61727 | |
| Activity | N/A | |
| Binding Conditions/Buffer | 150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature |
|
| Assay | N/A |
|
| PubMed ID | 22434135 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_479 | DNA | CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT | |
| Apta_480 | DNA | CGGCACACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT | |
| Apta_63 | DNA | GACCCGTGGTAGGGTAGGATGGGGTGGTC | |
| Apta_74 | DNA | GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC | |
| Apta_858 | DNA | GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG | |
| Apta_914 | DNA | CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT | |
| Apta_986 | DNA | CGTGTATCCCCTGTGTGTTTGTACTCGGCTACTGTATCCG | |
| Apta_1205 | DNA | GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG | |
| Apta_1255 | DNA | GGCGCATGATCATTCGGTGGGTAAGGTGGTGGTAACGTTG | |
| Apta_1257 | DNA | CGACGCACAGTCGCTGGTGCGTACCTGGTTGCCGTTGTGT | |
| Apta_1261 | DNA | GGGCGGACGCTAGGTGGTGATGCTGTGCTACACGTGTTGT | |
| Apta_782 | DNA | AGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAA | |
| Apta_750 | DNA | ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT | |
| Apta_143 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAGTTTCG | |
| Apta_41 | DNA | CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC | |
| Apta_69 | DNA | AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT | |
| Apta_71 | DNA | GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC | |
| Apta_72 | DNA | AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT | |
| Apta_180 | DNA | GGTCCCCCGTAAATTGAGAATTTGGGCGTTTTGGTTCTA | |
| Apta_648 | DNA | GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC |
DEV TOOL
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