AptaDB: Result

Interaction ID: 487

Aptamer sequence: AGCAAGGTTTGGTGTTGGTTGGTTGCTGGTTTTGGTTTC

Target unique ID: Q64962

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_482	N	Protein	RDRP_ASPVP	8 nM	PBS-T (10 mM Na2HPO4, 2 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, and 0.05% Triton-X; pH 7.5) with added 0.1 μg/ml poly-dIdC and 1 μg/ml BSA	20624933

Aptamer Sequence:	AGCAAGGTTTGGTGTTGGTTGGTTGCTGGTTTTGGTTTC
The optimal secondary structure in dot-bracket notation:	(((((.................)))))............
The centroid secondary structure in dot-bracket notation:	(((((.................)))))............

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_482	Description	N
Aptamer chemistry	DNA	Length	39 nt
GC content	46.2%	Molecular weight	12,158.77 Da
Molarity of 1 μg/μl solution	82.25 μM	Number of G-quadruplexes	2
G-Score	19	Function	Targeted binding
Sequence	AGCAAGGTTTGGTGTTGGTTGGTTGCTGGTTTTGGTTTC
Applications	Bound to ASPV

Type	Detail	Type	Detail
Uniprot ID	Q64962	Protein name	RNA replication polyprotein (ORF1 protein) [Cleaved into: Helicase (EC 3.6.4.13)] [Includes: Viral methyltransferase (EC 2.1.1.-); Putative Fe(2+) 2-oxoglutarate dioxygenase (EC 1.14.11.-); Protease (EC 3.4.22.-); RNA-directed RNA polymerase (EC 2.7.7.48)]
Gene name(s)	ORF1	Organism	RNA replication polyprotein (ORF1 protein) [Cleaved into: Helicase (EC 3.6.4.13)] [Includes: Viral methyltransferase (EC 2.1.1.-); Putative Fe(2+) 2-oxoglutarate dioxygenase (EC 1.14.11.-); Protease (EC 3.4.22.-); RNA-directed RNA polymerase (EC 2.7.7.48)]
Status	reviewed in uniprot database	Length	2183
Mass	247,262	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=ATP + H2O = ADP + H(+) + phosphate
PDB ID(s)	N/A
Function	RNA-directed RNA polymerase involved in viral RNA replication.

No relevant experimental diagram	Interaction ID	487
	Target type	Protein
	Target unique ID	Q64962
	Activity	8 nM
	Binding Conditions/Buffer	PBS-T (10 mM Na2HPO4, 2 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, and 0.05% Triton-X; pH 7.5) with added 0.1 μg/ml poly-dIdC and 1 μg/ml BSA
	Assay	N/A
	PubMed ID	20624933

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1205	DNA	GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG	69.23%
Apta_216	DNA	TGGTTGGTTTTTGGTTTTTTTTTTTGGTTT	64.10%
Apta_1085	DNA	TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC	61.54%
Apta_1247	DNA	GGTGGTTGCTGTTGTTTGTGGTTGGTGTGC	61.54%
Apta_750	DNA	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT	60.00%
Apta_913	DNA	GGCACTTGTTTGCTCGAACGATGGTTTGTGCTTTTGTGTT	60.00%
Apta_69	DNA	AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT	58.97%
Apta_1238	DNA	GGTTTTGTGTTTGTGTTTTGTGGTTGTTGT	58.97%
Apta_1241	DNA	GGTGTGGAGTGGTGTGTGTTGTTGTTTGTC	58.97%
Apta_1243	DNA	GGTGTTGTTGTTGGCTAGTTTGTTGTGGGC	58.97%
Apta_1245	DNA	AGTGTGAAGTGGTGTGTGTTGTTGTTTGTC	58.97%
Apta_858	DNA	GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG	57.50%
Apta_1186	DNA	AGGGGGTTTTGGTGGGGGGGGCTGGGTTGTCTTGGGGGTGGGCT	56.82%
Apta_71	DNA	GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC	56.41%
Apta_72	DNA	AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT	56.41%
Apta_74	DNA	GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC	56.41%
Apta_124	DNA	GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC	56.41%
Apta_350	DNA	GGTGGTGGTGGTTGTGGTGGTGGTGG	56.41%
Apta_364	DNA	TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT	56.41%
Apta_1176	DNA	AGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACT	56.41%

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